Metrika

  • citati u SCIndeksu: 0
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[]
  • posete u poslednjih 30 dana:7
  • preuzimanja u poslednjih 30 dana:5

Sadržaj

članak: 2 od 2  
Back povratak na rezultate
2018, vol. 45, br. 2, str. 203-211
Preliminarna karakterizacija vrste Bacillus subtilis kao probiotičkog dodatka ishrani prasadi
aNational Research Development Institute for Biology and Animal Nutrition (IBNA), Ilfov, Romania + University of Agronomic Sciences and Veterinary Medicine of Bucharest, Bucharest, Romania
bNational Research Development Institute for Biology and Animal Nutrition (IBNA) , Ilfov, Romania
cUniversity of Agronomic Sciences and Veterinary Medicine of Bucharest, Bucharest, Romania + Academy of Romanian Scientists, Bucharest, Romania

e-adresamihaela.dumitru22@yahoo.com
Projekat:
Project by Romanian Ministry of Research and Innovation through Program 1 - Development National Research-Development, Sub-program 1.2 - Institutional Performance - Projects funding excellence in R & D, Contract no. 17 PFE/ 17.10.2018 and Grant PN 18200103

Ključne reči: API 50 CHB; hemolitička aktivnost; enzimatski skrining
Sažetak
Cilj ovog istraživanja bio je karakterizacija bakterijske vrste Bacillus subtilis ATCC 6051a, kako bi se utvrdila mogućnost njene probiotičke primene u ishrani prasadi. U ovom istraživanju analizirane su morfološke, kulturalne i biohemijske karakteristike test soja, kao i njegova hemolitička i enzimska aktivnost (amilaza i proteaza). Identifikacija i analiza biohemijskih karakteristika test soja je sprovedena primenom katalaza testa, API identifikacioniog sistema (API 50 CHB Biomerieux) i primenu apiveb API 50 CHB V 4.0 softvera (B. subtilis, vrlo visok nivo identifikacije, 99,4% ID) uz ABIS online podršku. Hemolitička aktivnost test soja je sprovedena na krvnom agaru. Sposobnost rasta test soja je ispitana u statičkim uslovima (30 °C, 24 h, 1.36 x 108 CFU/ml) i uslovima konstantne agitacije (30 ° C, 24 h, 150 rpm, (1,6 k 109 CFU/ml ). Rezultati su pokazali da je test soj Gram-pozitivna štapićasta bakterija, sa štapićima raspoređenim u vidu kratkih lanaca ili nepravilnim parovima sa sposobnošću formiranja spora na hranljivom medijumu. Endospore su lokalizovane paracentralno i supterminalno ne uzrokujući deformaciju vegetativne ćelije. Testirani soj raste aerobno i ne pokazuje hemolitičku aktivnost. Enzimska aktivnost testiranog soja je utvrđivana kao pojava prosvetljenih zona oko same bakterijske kolonije. Na osnovu dobijenih rezultata može se zaključiti da ispitivani soj ima probiotičke osobine i može se nadalje koristiti za ispitivanje drugih probiotičkih osobina (otpornost pri pH 2.0, otpornost na žučne kiseline i soli, antibakterijska aktivnost, indukcija lokalnog imunološkog odgovora itd.).
Reference
Abdhul, K., Ganesh, M., Shanmughapriya, S., Vanithamani, S., Kanagavel, M., Anbarasu, K., Natarajaseenivasan, K. (2015) Bacteriocinogenic potential of a probiotic strain Bacillus coagulans [BDU3] from Ngari. International Journal of Biological Macromolecules, 79: 800-806
Abu, Z.A. (2018) Study the effect of probiotic bacteria isolated from foods on pathogens. Biomedical Research, 29(12): 2509-2515
Aruwa, C., Olatope, S. (2015) Characterization of Bacillus Species from Convenience Foods with Conventional and API Kit Method: A Comparative Analysis. Journal of Applied Life Sciences International, 3(1): 42-48
Breed, R.S., Murray, E.G.D., Smith, N.R. (1957) Bergey's manual of systematic bacteriology. Baltimore, Md, USA: Williams and Wilkins, Seventh Edition
Campbell, J.M., Crenshaw, J.D., Polo, J. (2013) The biological stress of early weaned piglets. Journal of Animal Science and Biotechnology, 4(1):
Cutting, S.M. (2011) Bacillus probiotics. Food Microbiology, 28(2): 214-220
Davis, M.E., Parrott, T., Brown, D.C., de Rodas, B.Z., Johnson, Z.B., Maxwell, C.V., Rehberger, T. (2008) Effect of a Bacillus-based direct-fed microbial feed supplement on growth performance and pen cleaning characteristics of growing-finishing pigs. Journal of Animal Science, 86(6): 1459-1467
Dumitru, M., Sorescu, I., Jurcoane, S., Câmpeanu, G., Tabuc, C., Hăbeanu, M. (2017) Assessing of morphological, cultural, biochemical profile and enzymatic activity of a Lactobacillus paracasei CCM 1837 strain. Academy of Romanian Scientists Annals: Series on Biological Sciences, 6 (2), 22-31
Elshaghabee, F.M.F., Rokana, N., Gulhane, R.D., Sharma, C., Panwar, H. (2017) Bacillus As Potential Probiotics: Status, Concerns, and Future Perspectives. Frontiers in Microbiology, 8:
FAO, WHO (2001) Health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria: Report of a Joint FAO/WHO Expert Consultation on Evaluation of Health and Nutritional Properties of Probiotics in Food including Powder Milk. u: Probiotics in food: Health and nutritional properties and guidelines for evaluation, FAO Food and Nutrition Paper, 85, 2006, WHO, FAO. (http://www.fao.org/3/a-a0512e.pdf)
FAO (2016) Probiotics in animal nutrition: Production, impact and regulation. FAO Animal Production and Health Paper, Rome, Italy, By Bajagai, Y.S. Klieve, A.V. Dart, P.J. Bryden, W.L. Ed. H.P.S. Makkar. 179, http://www.fao.org/3/a-i5933e.pdf
Guo, X., Li, D., Lu, W., Piao, X., Chen, X. (2006) Screening of Bacillus strains as potential probiotics and subsequent confirmation of the in vivo effectiveness of Bacillus subtilis MA139 in pigs. Antonie van Leeuwenhoek, 90(2): 139-146
Habeanu, M., Lefter, N., George, A., Tabuk, K., Dumitru, M., Ciruesku, G., Palade, M. (2017) Effects of dietary peas mixed with linseed (3:1) on the growth performance, enteritis and certain serum parameter in weaned piglets. Food and Feed Research, vol. 44, br. 2, str. 173-180
Hosoi, T., Ametani, A., Kiuchi, K., Kaminogawa, S. (2000) Improved growth and viability of lactobacilli in the presence of Bacillus subtilis ( natto ), catalase, or subtilisin. Canadian Journal of Microbiology, 46(10): 892-897
Jeon, H., Yang, S., Son, S., Kim, W., Lee, N., Paik, H. (2018) Evaluation of probiotic Bacillus subtilis P229 isolated from cheonggukjang and its application in soybean fermentation. LWT, 97: 94-99
Jung, J.H. (2012) Evaluation of the Probiotic Potential of Bacillus polyfermenticus CJ6 Isolated from Meju, a Korean Soybean Fermentation Starter. Journal of Microbiology and Biotechnology, 22(11): 1510-1517
Kaewtapee, C., Burbach, K., Tomforde, G., Hartinger, T., Camarinha-Silva, A., Heinritz, S., Seifert, J., Wiltafsky, M., Mosenthin, R., Rosenfelder-Kuon, P. (2017) Effect of Bacillus subtilis and Bacillus licheniformis supplementation in diets with low- and high-protein content on ileal crude protein and amino acid digestibility and intestinal microbiota composition of growing pigs. Journal of Animal Science and Biotechnology, 8(1):
Lee, J., Park, I., Choi, Y., Cho, J. (2012) Bacillus strains as feed additives: In vitro evaluation of its potential probiotic properties. Revista Colombiana de Ciencias Pecuarias, 577-585; 25
Lei, Y., Kim, I.H. (2014) Effect of Phaffia rhodozyma on performance, nutrient digestibility, blood characteristics, and meat quality in finishing pigs. Journal of Animal Science, 92(1): 171-176
Leser, T.D., Knarreborg, A., Worm, J. (2008) Germination and outgrowth of Bacillus subtilis and Bacillus licheniformis spores in the gastrointestinal tract of pigs. Journal of Applied Microbiology, 104(4): 1025-1033
Link, R., Kováč, G. (2006) The effect of probiotic BioPlus 2B on feed efficiency and metabolic parameters in swine. Biologia, 61(6): 783-787
Maneewan, C., Yamauchi, K., Thirabunyanon, M., Siri, S., Mekbungwan, A., Thongwittaya, N. (2011) Development of Bacillus subtilis MP and effective utilization on productivity and microorganisms in feces of suckling piglets. International Journal of Applied Research in Veterinary Medicine, 9 (4): 382-387
Markowiak, P., Śliżewska, K. (2018) The role of probiotics, prebiotics and synbiotics in animal nutrition. Gut Pathogens, 10(1):
Meng, Q.W., Yan, L., Ao, X., Zhou, T.X., Wang, J.P., Lee, J.H., Kim, I.H. (2010) Influence of probiotics in different energy and nutrient density diets on growth performance, nutrient digestibility, meat quality, and blood characteristics in growing-finishing pigs. Journal of Animal Science, 88(10): 3320-3326
Merchant, H.A., McConnell, E.L., Liu, F., Ramaswamy, C., Kulkarni, R.P., Basit, A.W., Murdan, S. (2011) Assessment of gastrointestinal pH, fluid and lymphoid tissue in the guinea pig, rabbit and pig, and implications for their use in drug development. European Journal of Pharmaceutical Sciences, 42(1-2): 3-10
Mishra, S., Behera, N. (2018) Amylase activity of a starch degrading bacteria isolated from soil receiving kitchen wastes. African Journal of Biotechnology, 7 (18): 3326-3331
Nagaraju, E.V., Divakar, G. (2012) Screening and characterization of protease producing Bacillus spp. from spoiled vegetables and fruits. International Journal of Advances in Pharmacy, Biology and Chemistry, 1 (4): 485-488
Ritter, A.C., Correa, F.A.P., Veras, F.F., Brandelli, A. (2018) Characterization of Bacillus subtilis available as probiotics. Journal of Microbiology Research, 8 (2): 23-32
Sanchez, O.A.C., Luna, G.A., Campa, C.A.I., Escamilla, M.R., Flores, M.M.del C., Mazon, S.J.M. (2015) Isolation and characterization of potential probiotic bacteria from pustulose ark (Anadara tuberculosa) suitable for shrimp farming. Latin American Journal of Aquatic Research, 43(1): 123-136
Şeker, E. (2010) Identification of Candida Species Isolated from Bovine Mastitic Milk and Their In Vitro Hemolytic Activity in Western Turkey. Mycopathologia, 169(4): 303-308
Siddalingeshwara, K.G., Huchesh, C.H., Puttaraju, H.P., Karthic, J., Sudipta, K.M., Pramod, T., Vishwanatha, T. (2010) Screening and characterization of protease from Bacillus sp. International Journal of Applied Biology and Pharmaceutical Technology, 1 (2): 575-581
Singh, V., Sharma, R., Sharma, P. (2015) Isolation, screening and optimization of amylase producing Bacillus sp. from soil. Asian Pacific Journal of Health Sciences, 2(3): 86-93
Sorokulova, I.B., Pinchuk, I.V., Denayrolles, M., Osipova, I.G., Huang, J.M., Cutting, S.M., Urdaci, M.C. (2008) The Safety of Two Bacillus Probiotic Strains for Human Use. Digestive Diseases and Sciences, 53(4): 954-963
Stoica, C., Sorescu, I. (2017) ABIS online: Advanced Bacterial Identification Software, an original tool for phenotypic bacterial identification. Regnum Prokaryotae (www.tgw1916.net)
Vishwanatha, T., Spoorthi, N.J., Reena, V., Divyashree, B.C., Siddalingeshwara, K.G., Karthic, J., Sudipta, K.M. (2010) Screening of substrates for protease production from Bacillus licheniformis. International Journal of Engineering Science and Technology, 2 (11): 6550-6554
Yang, F., Hou, C., Zeng, X., Qiao, S. (2015) The Use of Lactic Acid Bacteria as a Probiotic in Swine Diets. Pathogens, 4(1): 34-45
Yirga, H. (2015) The Use of Probiotics in Animal Nutrition. Journal of Probiotics & Health, 03(02):
 

O članku

jezik rada: engleski
vrsta rada: originalan članak
DOI: 10.5937/FFR1802203D
objavljen u SCIndeksu: 01.02.2019.
Creative Commons License 4.0