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2020, br. 40, str. 5-12
Antifungal activity of aqueous and corn steep liquor extract of Ficus exasperata, Anonna muricata and Azadiractha indica
(naslov ne postoji na srpskom)
aFederal University of Agriculture, College of Bioscience, Abeokuta, Ogun State, Nigeria
bForestry Research Institute of Nigeria, Biomedicinal Research Centre, Jericho Hills, Ibadan, Nigeria

Ključne reči: Candida species; Ficus exasperata; Annona muricata; Azadirachta indica; Corn Steep Liquor; Antifungal activity
(ne postoji na srpskom)
This study investigated the activity of aqueous and corn steep liquor (CSL) extracts of Ficus exaperasta, Annona muricata and Azadirachta indica against Candida spp. isolated from high vaginal swab samples. Phytochemical screening of the plants was done using standard methods, the antifungal activity of the plant's extracts and standard drugs were tested against isolates of Candida spp. using the agar well diffusion method; the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) were also determined using microdilution standardized techniques. Phytochemical screening of the aqueous and CSL extracts of the plants revealed the presence of tannin, saponin, flavonoids, and terpenoids. Among the five Candida strain isolates, the zone of inhibition produced by the plant extracts against C. albicans shows a range of 0-18.3 mm; C. krusei (strain A): 5.1-24.5 mm; C. krusei (strain B): 0-18.0 mm; C. kefyr (strain A): 6.1-27.5 mm; and C. kefyr (strain B): 0-22.0 mm. The CSL extract had higher inhibitory action compared with aqueous extract, also F. exasperata and A. muricata gave better antifungal activity against the tested Candida strains. The MIC of the aqueous and CSL extracts of the F. exasperata ranged between 6.25-12.50 mg/mL; A. muricata: 3.125-12.500 mg/mL, while the aqueous and CSL extracts of A. indica was found to have no activity at all the tested concentrations against C. albicans, C. krusei (strain A) and C. krusei (strain B), similar observation for the MFC. This study proved the antifungal efficacy of aqueous and CSL extracts of F. exasperata, A. muricata, and A. indica against isolates of Candida species which are usually implicated in candidiasis.
Abdus-Salaam, R.B., Adepoju, P.A., Olaleye, O.N., Adeoye, I.A. (2014) Studies on the antimicrobial effect of corn steep liquor on some diarrhoea causing organisms. African Journal of Biotechnology, 13(2): 332-335
Adebayo, E., Ishola, O., Taiwo, O., Majolagbe, O., Adekeye, B. (2009) Evaluations of the methanol extract of Ficus exasperata stem bark, leaf and root for phytochemical analysis and antimicrobial activities. African Journal of Plant Science, 3(12): 283-287
Ajayi, O.B., Oluyege, J.O., Olalemi, O.M., Ilesanmi, T.M. (2012) Nutritional composition, phytochemical screening and antimicrobial properties of the leaf of Ficus exasperata (Vahl). Asian Journal of Biological and Life Sciences, 1(3): 242-246
Asticcioli, S.L., Sacco, L., Daturi, R., Matti, C., Nucleo, E., Zara, F., Pagani, L. (2002) Trends in frequency and in vitro antifungal susceptibility patterns of Candida isolates from women attending the STD outpatients clinic of a tertiary care hospital in Northern Italy during the years 2002-2007. New Microbiologica, 32(2): 199-204
Bader, T., Bodendorfer, B., Schröppel, K., Morschhäuser, J. (2003) Calcineurin Is Essential for Virulence in Candida albicans. Infection and Immunity, 71(9): 5344-5354
Bafor, E.E., Igbinuwen, O. (2009) Acute toxicity studies of the leaf extract of Ficus exasperata on haematological parameters, body weight and body temperature. Journal of Ethnopharmacology, 123(2): 302-307
Barua, D., Basavanna, J., Varghese, R. (2017) Efficacy of Neem Extract and Three Antimicrobial Agents Incorporated into Tissue Conditioner in Inhibiting the Growth of C. Albicans and S. Mutans. Journal of clinical and diagnostic research, 11(5): 97-101
Calderone, R.A., Fonzi, W.A. (2001) Virulence factors of Candida albicans. Trends in Microbiology, 9(7): 327-335
Choudhary, S., Dwivedi, A.K., Singh, P. (2017) Antifungal activities of neem leaf extracts on the growth of Macrophomina phaseolina in vitro and its chemical characterization. IOSR Journal of Environmental Science, Toxicology and Food Technology, 11(4): 44-48
Coleman, J.J., Okoli, I., Tegos, G.P., Holson, E.B., Wagner, F.F., Hamblin, M.R., Mylonakis, E. (2010) Characterization of plant-derived saponin natural products against Candida albicans. ACS Chemical Biology, 5(3): 321-332
Ellabib, M.S., Eljariny, I.A. (2001) In vitro activity of 6 antifungal agents on Candida species isolated as causative agents from vaginal and other clinical specimens. Saudi Journal of Medicine, 22(10): 860-863
Galle, L.C., Gianinni, M.J.S.M. (2004) Prevalência e susceptibilidade de leveduras vaginais. Jornal Brasileiro de Patologia e Medicina Laboratorial, 40(4): 229-236
Hamza, O.J.M., Matee, M.I.N., Moshi, M.J., Simon, E.N.M., Mugusi, F., Mikx, F.H.M., Helderman, W., Rijs, A.J.M.M., van der Ven, A.J.A.M., Verweij, P.E. (2008) Species distribution and in vitro antifungal susceptibility of oral yeast isolates from Tanzanian HIV-infected patients with primary and recurrent oropharyngeal candidiasis. BMC Microbiology, 8(1): 135-135
Harborne, J.B. (1973) Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Netherlands: Springer
Hutchinson, J., Dalziel, J. (2000) Flora of West Tropical Africa. Kew: Royal Botanic Gardens, Volume 1 Part 2
Itelima, J., Nwokedi, V., Ogbonna, A., Nyam, M. (2016) Phytochemical screening and antimicrobial activity evaluation of aqueous and ethanolic extracts of the leaf of Azadirachta indica Juss (neem) on some microorganisms. World Journal of Microbiology, 3(1): 56-60
Iyanda-Joel, W.O., Omonigbehin, E.A., Iweala, E.E.J., Chinedu, S.N. (2019) Antibacterial studies on fruit-skin and leaf extracts of Annona muricata in Ota, Nigeria. IOP Conference Series: Earth and Environmental Science, 331: 012029-012029
Kanthasamy, A., Subramanian, S., Govindasamy, S. (1989) Bactericidal and fungicidal effects of Prosopis juliflora alkaloidal fraction. Indian Drugs, 26: 390-394
Kareem, K., Kareem, S., Adeyemo, O., Egberongbe, R. (2010) In vitro antimicrobial properties of Bridelia ferruginea on some clinical isolates. Agriculture and Biology Journal of North America, 1(3): 416-420
Kothavade, R.J., Kura, M.M., Valand, A.G., Panthaki, M.H. (2010) Candida tropicalis: Its prevalence, pathogenicity and increasing resistance to fluconazole. Journal of Medical Microbiology, 59(8): 873-880
Latté, K.P., Kolodziej, H. (2000) Antifungal Effects of Hydrolysable Tannins and Related Compounds on Dermatophytes, Mould Fungi and Yeasts. Zeitschrift für Naturforschung C, 55(5-6): 467-472
Lawal, I., Borokini, T., Oyeleye, A., Williams, O., Olayemi, J. (2012) Evaluation of extract of Ficus exasperata Vahl. root bark for antimicrobial activities against some strains of clinical isolates of bacterial and fungi. International Journal of Modern Botany, 2(1): 6-12
Levinson, W.E. (2016) Review of Medical Microbiology and Immunology. McGraw-Hill Education, 14E, Google-Books-ID: TPnnCwAAQBAJ
Levy, S.B. (2002) Factors impacting on the problem of antibiotic resistance. Journal of Antimicrobial Chemotherapy, 49(1): 25-30
Mahmoud, D.A., Hassanein, N.M., Youssef, K.A., Abou, Z.M.A. (2011) Antifungal activity of different neem leaf extracts and the nimonol against some important human pathogens. Brazilian Journal of Microbiology, 42(3): 1007-1016
Malwal, M., Sarin, R. (2011) Antimicrobial efficacy of Murraya koenigii (Linn.) Spreng. root extracts. Indian Journal of Natural Products and Resources, 2(1): 48-51
McLaughlin, J.L. (2008) Paw paw and cancer: Annonaceous acetogenins from discovery to commercial products. Journal of Natural Products, 71(7): 1311-1321
Mohammed, H.A., Omer, A.F.A. (2017) Antibacterial activity of Azadirachta indica (neem) leaf extract against bacterial pathogens in Sudan. American Journal of Research and Communication, 3(5): 246-251
Mohanty, S., Xess, I., Hasan, F., Kapil, A., Mittal, S., Tolosa, J.E. (2007) Prevalence & susceptibility to fluconazole of Candida species causing vulvovaginitis. Indian Journal of Medical Research, 126(3): 216-219
Mondall, N., Mojumdar, A., Chatterje, S., Banerjee, A., Datta, J., Gupta, S. (2009) Antifungal activities and chemical characterization of neem leaf extracts on the growth of some selected fungal species in vitro culture medium. Journal of Applied Science and Environmental Management, 13(1): 49-53
Morey, A.T., de Souza, F.C., Santos, J.P., Pereira, C.A., Cardoso, J.D., de Almeida, R.S., Costa, M.A., de Mello, J.C., Nakamura, C.V., Pinge-Filho, P., Yamauchi, L.M., Yamada-Ogatta, S.F. (2016) Antifungal activity of condensed tannins from Stryphnodendron adstringens: Effect on Candida tropicalis growth and adhesion properties. Current Pharmaceutical Biotechnology, 17(4): 365-375
Nahak, G., Sahu, R.K. (2010) In vitro antioxidative acitivity of Azadirachta indica and Melia azedarach leaves by DPPH scavenging assay. Journal of American Science, 6(6): 123-128
Nimenibo-Uadia, R. (2017) Chemical composition and phytochemical screening of Ficus exasperata (Vahl) leaf. Nigerian Journal of Pharmaceutical and Applied Science Research, 6(1): 12-18
Obasi, N., Egbuonu, A., Ukoha, P., Ejikeme, P. (2010) Comparative phytochemical and antimicrobial screening of some solvent extracts of Samanea saman pods. African Journal of Pure and Applied Chemistry, 4(9): 206-212
Oberlies, N.H., Jones, J.L., Corbett, T.H., Fotopoulos, S.S., Mclaughlin, J.L. (1995) Tumor cell growth inhibition by several Annonaceous acetogenins in an in vitro disk diffusion assay. Cancer Letters, 96(1): 55-62
Olaiya, C., Idowu, P., Karigidi, K. (2016) Antioxidative and antimicrobial activities of corn steep liquor anti-diabetic herb extracts. Annals. Food Science and Technology, 17(2): 272-279
Pai, M.B.H., Prashant, G.M., Murlikrishna, K.S., Shivakumar, K.M., Chandu, G.N. (2010) Antifungal efficacy of Punica granatum, Acacia nilotica, Cuminum cyminum and Foeniculum vulgare on Candida albicans: An in vitro study. Indian Journal of Dental Research: Official Publication of Indian Society for Dental Research, 21(3): 334-336
Patuwo, C., Pasetto, S., Youngr, K., Murata, M. (2014) Antifungal activity of flavonoids against Candida albicans. u: Annual Meeting & Exhibition of the AADR (43rd)
Pfaller, M.A., Diekema, D.J. (2007) Epidemiology of Invasive Candidiasis: A Persistent Public Health Problem. Clinical Microbiology Reviews, 20(1): 133-163
Pierce, G.E. (2005) Pseudomonas aeruginosa, Candida albicans, and device-related nosocomial infections: Implications, trends, and potential approaches for control. Journal of Industrial Microbiology & Biotechnology, 32(7): 309-318
Pinto, E., Vale-Silva, L., Cavaleiro, C., Salgueiro, L. (2009) Antifungal activity of the clove essential oil from Syzygium aromaticum on Candida, Aspergillus and dermatophyte species. Journal of Medical Microbiology, 58(11): 1454-1462
Prusti, A., Mishea, S., Sahoo, S., Mishea, S. (2008) Antibacterial activity of some Indian medicinal plants. Ethanobotanical leaflets, 12: 227-228
Rahman, A., Samoylenko, V., Jacob, M., Sahu, R., Jain, S., Khan, S., Tekwani, B., Muhammad, I. (2011) Antiparasitic and antimicrobial indolizidines from the leaves of Prosopis glandulosa var. glandulosa. Planta Medica, 77(14): 1639-1643
Roopashree, T.S., Dang, R., Rani, R.S., Narendra, C. (2008) Antibacterial activity of antipsoriatic herbs: Cassia tora, Momordica charantia and Calendula officinalis. International Journal of Applied Research in Natural Products, 1(2): 20-28
Salazar-Aranda, R., Granados-Guzmán, G., Pérez-Meseguer, J., González, G., de Torres, N. (2015) Activity of Polyphenolic Compounds against Candida glabrata. Molecules, 20(10): 17903-17912
Saleh, A.Z.S., Hossain, M.A. (2016) Biological activities of different neem leaf crude extracts used locally in Ayurvedic medicine. Pacific Science Review A: Natural Science and Engineering, 18(2): 128-131
Shah, S., Nasreen, S., Sheikh, P.A. (2012) Cultural and morphological characterization of Trichoderma spp. associated with green mold disease of Pleurotus spp. in Kashmir. Research Journal of Microbiology, 7(2): 139-144
Sonibare, M.A., Ogunwande, I.A., Walker, T.M., Setzer, W.N., Soladoye, M.O., Essien, E. (2006) Volatile Constituents of Ficus Exasperata Leaves. Natural Product Communications, 1(9): 1934578X0600100-1934578X0600100
Takon, I. (2013) In-vitro evaluation of antibacterial activity of ethanolic and aqueous extracts of Ficus exasperata vahl. (Moraceae) leaves. Journal of Microbiology and Biotechnology Research, 3(4): 7-12
Tsai, P., Chen, Y., Hsu, P., Lan, C. (2013) Study of Candida albicans and its interactions with the host: A mini review. BioMedicine, 3(1): 51-64
Tsuzuki, J.K., Svidzinski, T.I.E., Shinobu, C.S., Silva, L.F.A., Rodrigues-Filho, E., Cortez, D.A.G., Ferreira, I.C.P. (2007) Antifungal activity of the extracts and saponins from Sapindus saponaria L. Anais da Academia Brasileira de Ciências, 79(4): 577-583
Vijayameena, C., Subhashini, G., Loganayagi, M., Ramesh, B. (2013) Phytochemical screening and assessment of antibacterial activity for the bioactive compounds in Annona muricata. International Journal of Current Microbiology and Applied Sciences, 2(1): 1-8
Yang, C., Zhang, Y., Jacob, M.R., Khan, S.I., Zhang, Y., Li, X. (2006) Antifungal Activity of C-27 Steroidal Saponins. Antimicrobial Agents and Chemotherapy, 50(5): 1710-1714
Yang, L., Liu, X., Zhuang, X., Feng, X., Zhong, L., Ma, T. (2018) Antifungal Effects of Saponin Extract from Rhizomes of Dioscorea panthaica Prain et Burk against Candida albicans. Evidence-Based Complementary and Alternative Medicine, 2018: 1-13, Article ID 6095307

O članku

jezik rada: engleski
vrsta rada: istraživanja
DOI: 10.5937/leksir2040005A
primljen: 03.04.2020.
prihvaćen: 19.05.2020.
objavljen onlajn: 14.09.2020.
objavljen: 25.12.2020.
objavljen u SCIndeksu: 31.12.2020.
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