• citations in SCIndeks: 0
  • citations in CrossRef:0
  • citations in Google Scholar:[]
  • visits in previous 30 days:3
  • full-text downloads in 30 days:0


article: 1 from 15  
Back back to result list
2022, vol. 37, iss. 2, pp. 49-62
Fusarium species associated with wheat head blight disease in Algeria: Characterization and effects of triazole fungicides
aUniversity of BBA, Department of Biology, FSNV-STU, Laboratory of Characterization and Valorisation of Natural Resources, Algeria
bFaculty FSNV -UFAS Setif, Department of Microbiology, Laboratory of Applied Microbiology, Algeria
cFaculty FSNV -UFAS Setif, Department of Agronomy, Laboratory of Applied Microbiology, Algeria
Fusarium head blight is an important disease of durum wheat which requires several fungicide treatments of seeds to achieve satisfactory control. The current study was carried out to evaluate commercially available fungicides in vitro for their efficacy against eighteen Fusarium spp. isolates collected from different fields in the northeastern part of Algeria. The morphological and molecular characterization reveals the presence in wheat seeds of the main species complexes F. acuminatum, F. equiseti, F. avenaceum, F. solani, F. culomorum, F. incarnatum-equiseti, as well as F. tricinctum species complex and F. chlamydosporum species complex. Antifungal activity of fungicides shows that all triazoles tested have proven their effectiveness in inhibiting the mycelial growth of various strains of Fusarium tested. However, their sensitivity varies between them significantly (p<0.05) depending on the dose applied and period of exposure to each fungicide. The results showed that tebuconazole (Raxil and Tébuzole) and the combination fludioxonil + difenoconazole greatly reduced the mycelial growth of Fusarium isolates by 84.31%, 82.94%, 81.33%, respectively, as compared to difenoconazole alone (73.16%) at the recommended dose after five days of exposure. Regarding their effect on conidia germination, tebuconazole was more effective than fludioxonil + difenoconazole, which leads to deformation of cell wall structure and fragmentation of conidia. These results will provide useful information to select suitable fungicides for seed treatment and management of wheat head blight disease.
*** (2008) Detection et identification des espèces de Fusarium spp. et Microdochium nivale sur grains de céréales par isolement mycologique semi sélectif et étude microbiologique: Ref. MH-03.16: version B. (LSV) -station de Malzéville. France, pp 1-25. (In French)
Abdallah-Nekache, N., Laraba, I., Ducos, C., Barreau, C., Bouznad, Z., Boureghda, H. (2019) Occurrence of Fusarium head blight and Fusarium crown rot in Algerian wheat: Identification of associated species and assessment of aggressiveness. European Journal of Plant Pathology, 154(3): 499-512
Askarne, L., Talibi, I., Boubaker, H., Boudyach, E.H., Msanda, F., Saadi, B., Ait, B.A.A., et al. (2012) In vitro and in vivo antifungal activity of several Moroccan plants against Penicillium italicum, the causal agent of citrus blue mold. Crop Protection, 40, 53-58
Becher, R., Weihmann, F., Deising, H.B., Wirsel, S.G.R. (2011) Development of a novel multiplex DNA microarray for Fusarium graminearum and analysis of azole fungicide responses. BMC Genomics, 12, 52
Bhimani, M.D., Golakiya, B.B., Akbari, L.F. (2018) Evaluation of different fungicides against fenugreek wilt (Fusarium oxysporum Schlecht). International Journal of Chemical Studies, 6(2): 29-34
Bottalico, A., Perrone, G. (2002) Toxigenic Fusarium species and mycotoxins associated with head blight in small-grain cereals in Europe. European Journal of Plant Pathology, 108(7), 611-624
Dahl, B., Wilson, W.W. (2018) Risk premiums due to Fusarium Head Blight (FHB) in wheat and barley. Agricultural Systems, 162: 145-153
Falcão, V.C.A., Ono, M.A., de Ávila, M.T., Vizoni, E., Hirooka, E.Y., Ono, E.Y.S. (2011) Fusarium verticillioides: Evaluation of fumonisin production and effect of fungicides on in vitro inhibition of mycelial growth. Mycopathologia, 171(1): 77-84
FAO (2022) Food and agriculture organization of the UN: World food situation. (accessed on 15 February 2022)
Godet, F., Limpert, E. (1998) Recent evolution of multiple resistance of Blumeria (Erysiphe) graminis f.sp. tritici to selected DMI and morpholine fungicides in France. Pesticide Sciences, 54(3): 2-8
Goswami, R.S., Kistler, H. (2004) Heading for disaster: Fusarium graminearum on cereal crops. Molecular Plant Pathology, 5(6): 515-525
Gxasheka, M., Wang, J., Gunya, B., Mbanjwa, V., Tyasi, T.L., Dlamini, P., Gao, J. (2021) In vitro effect of some commercial fungicides on mycelial growth of Fusarium species causing maize ear rot disease in China. Archives of Phytopathology and Plant Protection, 54(11-12): 557-569
Hadjout, S., Chéreau, S., Mekliche, L., Marchegay, G., Ducos, C., Boureghda, H., Zouidi, M., Barreau, C., Bouznad, Z., Richard-Forget, F. (2022) Molecular identification of some Fusarium isolates and their chemotypes involved in fusarium head blight on Durum wheat in Algeria. Archives of Phytopathology and Plant Protection, 55(4): 499-513
Haidukowski, M., Visconti, A., Perrone, G., Vanadia, S., Pancaldi, D., Covarelli, L., Pascale, M., et al. (2012) Effect of prothioconazole-based fungicides on Fusarium head blight, grain yield and deoxynivalenol accumulation in wheatunder field conditions. Phytopathologia Mediterranea, 51(1), 236-246
Hellin, P., King, R., Urban, M., Hammond-Kosack, K.E., Legrève, A. (2018) The adaptation of Fusarium culmorum to DMI fungicides is mediated by major transcriptome modifications in response to azole fungicide, including the overexpression of a pdr transporter (FcABC1). Frontiers in Microbiology, 9, 1385
IBM Corp (2017) IBM SPSS statistics for Windows: Version 25.0. Armonk, NY
Kazan, K., Gardiner, D.M., Manners, J.M. (2012) On the trail of a cereal killer: Recent advances in Fusarium graminearum pathogenomics and host resistance. Molecular Plant Pathology, 13(4): 399-413
Kim, J.H., Campbell, B.C., Mahoney, N., Chan, K.L., Molyneux, R.J., May, G.S. (2007) Enhancement of fludioxonil fungicidal activity by disrupting cellular glutathione homeostasis with 2,5-dihydroxybenzoic acid. FEMS Microbiology Letters, 270(2): 284-290
Klix, M.B., Verreet, J.A., Beyer, M. (2007) Comparison of the declining triazole sensitivity of Gibberella zeae and increased sensitivity achieved by advances in triazole fungicide development. Crop Protection, 26(4): 683-690
Klosowski, A.C., Castellar, C., Stammler, G., May, D.M.L.L. (2018) Fungicide sensitivity and monocyclic parameters related to thePhakopsora pachyrhizi-soybean pathosystem from organic and conventional soybean production systems. Plant Pathology, 67(8): 1697-1705
Köller, W., Wilcox, W.F., Barnard, J., Jones, A.L., Braun, P.G. (1997) Detection and quantification of resistance of venturia inaequalis populations to sterol demethylation inhibitors. Phytopathology, 87(2): 184-90
Kotowicz, N.K., Frac, M., Lipiec, J. (2014) The importance of Fusarium fungi in wheat cultivation -pathogenicity and mycotoxins production: A review. Journal of Animal and Plant Sciences, 21(2): 3326-3343
Leroux, P., Albertini, C., Gautier, A., Gredt, M., Walker, A.S. (2007) Mutations in theCYP51 gene correlated with changes in sensitivity to sterol 14a-demethylation inhibitors in field isolates of Mycosphaerella graminicola. Pest Management Science, 63(7): 688-698
Leslie, J.F., Summerell, B.A. (2006) The Fusarium laboratory manual. Ames, USA: Wiley, 388-388
Li, X., Sheng, L., Sbodio, A.O., Zhang, Z., Sun, G., Blanco-Ulate, B., Wang, L. (2022) Photodynamic control of fungicide-resistant Penicillium digitatum by vitamin K3 water-soluble analogue. Food Control, 135: 108807-108807
Liddell, C.M. (2003) Systematics of Fusarium species and allies associated with Fusarium head blight. in: Leonard, K.J.; Bushnell, W.R. [ed.] Fusarium head blight of wheat and barley, St. Paul, MN, USA: APS Press, 35-43
Lu, X.H., Michael, D.R., Livingston, S., Nunez, J., Hao, J.J. (2012) Fungicide sensitivity of Pythium spp. associated with cavity spot of carrot in California and Michigan. Plant Disease, 96(3): 384-388
Ma, Z., Michailides, T. (2005) Advances in understanding molecular mechanisms of fungicide resistance and molecular detection of resistant genotypes in phytopathogenic fungi. Crop Protection, 24(10): 853-863
Malbrán, I., Mourelos, C.A., Pardi, M., Oufensou, S., Balmas, V., Delogu, G., Migheli, Q., Lori, G.A., Juárez, M., Girotti, J.R. (2020) Commercially available natural inhibitors of trichothecene production in Fusarium graminearum: A strategy to manage Fusarium head blight of wheat. Crop Protection, 138: 105313-105313
Masiello, M., Somma, S., Ghionna, V., Logrieco, A.F., Moretti, A. (2019) In vitro and in field response of different fungicides against aspergillus flavus and Fusarium species causing ear rot disease of maize. Toxins, 11(1): 11-11
Mateo, E.M., Valle-Algarra, F.M., Jiménez, M., Magan, N. (2013) Impact of three sterol-biosynthesis inhibitors on growth of Fusarium langsethiae and on T-2 and HT-2 toxin production in oat grain under different ecological conditions. Food Control, 34(2): 521-529
Mateo, E.M., Valle-Algarra, F.M., Mateo, R.M., Jiménez, M., Magan, N. (2011) Effect of fenpropimorph, prochloraz and tebuconazole on growth and production of T-2 and HT-2 toxins by Fusarium langsethiae in oat-based medium. International Journal of Food Microbiology, 151(3): 289-298
Mavroeidi, V.I., Shaw, M.W. (2005) Sensitivity distributions and cross-resistance patterns of Mycosphaerella graminicola to fluquinconazole, prochloraz and azoxystrobin over a period of 9 years. Crop Protection, 24(3): 259-266
Nakajima, T. (2010) Fungicides application against Fusarium head blight in wheat and barley for ensuring food safety. in: Carisse, O. [ed.] Fungicides, London, UK: Intech Open, 140-156
Nene, Y.L., Thapliyal, P.N. (1993) Fungicides in plant disease control. New Delhi, India: Oxford and IBH Publication Company
Nielsen, L.K., Jensen, J.D., Nielsen, G.C., Jensen, J.E., Spliid, N.H., Thomsen, I.K., Justesen, A.F., Collinge, D.B., Jørgensen, L.N. (2011) Fusarium head blight of cereals in Denmark: Species complex and related mycotoxins. Phytopathology®, 101(8): 960-969
Ochiai, N., Fujimura, M., Oshima, M., Motoyama, T., Ichiishi, A., Yamada-Okabe, H., Yamaguchi, I. (2002) Effects of iprodione and fludioxonil on glycerol synthesis and hyphal development in candida albicans. Bioscience, Biotechnology, and Biochemistry, 66(10): 2209-2215
O'donnell, K., Kistler, H.C., Tacke, B.K., Casper, H.H. (2000) Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab. Proceedings of the National Academy of Sciences, 97(14): 7905-7910
O'donnell, K., Ward, T.J., Geiser, D.M., Kiestler, H.C., Aoki, T. (2004) Genealogical concordance between mating type locus and seven other nuclear genes supports formal recognition of nine phylogenetically distinct species within the Fusarium graminearum clade. Fungal Genetics and Biology, 41: 600-623
Paul, P.A., Lipps, P.E., Hershman, D.E., McMullen, M.P., Draper, M.A., Madden, L.V. (2008) Efficacy of triazole-based fungicides for Fusarium head blight and deoxynivalenol control in wheat: A multivariate meta-analysis. Phytopathology®, 98(9): 999-1011
Paul, P.A., McMullen, M.P., Hershman, D.E., Madden, L.V. (2010) Meta-analysis of the effects of triazole-based fungicides on wheat yield and test weight as influenced by Fusarium head blight intensity. Phytopathology®, 100(2): 160-171
Pereira, C.B., Ward, T.J., Del, P.E.M., Mara, M.G., Busman, M., McCormick, S.P., Feksa, H.R., de Almeida, J.L., Tessmann, D.J. (2021) Five-year survey uncovers extensive diversity and temporal fluctuations among fusarium head blight pathogens of wheat and barley in Brazil. Plant Pathology, 70(2): 426-435
Petronaitis, T., Simpfendorfer, S., Hüberli, D. (2021) Importance of Fusarium spp. In wheat to food security: A global perspective. in: Strange, P.R.; Korsten, L.; Lodovica Gullino, M. [ed.] Plant diseases and food security in the 21st century, Cham, Switzerland: Springer International Publishing, 127-159
Proctor, R.H., McCormick, S.P., Alexander, N.J., Desjardins, A.E. (2009) Evidence that a secondary metabolic biosynthetic gene cluster has grown by gene relocation during evolution of the filamentous fungus Fusarium. Molecular Microbiology, 74(5): 1128-1142
Ramirez, M.L., Chulze, S., Magan, N. (2004) Impact of environmental factors and fungicides on growth and deoxinivalenol production by Fusarium graminearum isolates from Argentinian wheat. Crop Protection, 23(2): 117-125
Rojas, E.C., Jensen, B., Jørgensen, H.J.L., Latz, M.A.C., Esteban, P., Ding, Y., Collinge, D.B. (2020) Selection of fungal endophytes with biocontrol potential against Fusarium head blight in wheat. Biological Control, 144: 104222-104222
Rosslenbroich, H.J., Stuebler, D. (2000) Botrytis cinerea: History of chemical control and novel fungicides for its management. Crop Protection, 19(8-10), 557-561
Sharifi, K., Zare, R., Zamanizadeh, H.R., Mirabolfathy, M., Rezaee, S. (2016) Identification of Fusarium species associated with Fusarium head blight of wheat in the North of Iran and phylogenetic analysis of the dominant species. Rostaniha, 17(2): 173-187
Shcherbakova, L., Odintsova, T., Pasechnik, T., Arslanova, L., Smetanina, T., Kartashov, M., Slezina, M., Dzhavakhiya, V. (2020) Fragments of a wheat hevein-like antimicrobial peptide augment the inhibitory effect of a triazole fungicide on spore germination of Fusarium oxysporum and alternaria solani. Antibiotics, 9(12): 870-870
Shikur, G.E., Sharma-Poudyal, D., Paulitz, T.C., Erginbas-Orakci, G., Karakaya, A., Dababat, A.A. (2018) Identity and pathogenicity of Fusarium species associated with crown rot on wheat (Triticum spp.) in Turkey. European Journal of Plant Pathology, 150(2): 387-399
Stehman, C., de Waard, M.A. (1996) Sensitivity of populations of Botrytis cinerea to triazoles, benomyl and vinclozolin. European Journal of Plant Pathology, 102, 171-180
Su, Y.Y., Qi, Y.L., Cai, L. (2012) Induction of sporulation in plant pathogenic fungi. Mycology, 3(3): 195-200
Suty-Heinze, A., Dutzmann, S. (2004) Fusarium head blight: An additional strength of prothioconazole. Pflanzenschutz-Nachrichten Bayer, 57,(2),265-282
Tittlemier, S.A., Roscoe, M., Trelka, R., Gaba, D., Chan, J.M., Patrick, S.K., Sulyok, M., Krska, R., McKendry, T., Gräfenhan, T. (2013) Fusarium damage in small cereal grains from western Canada: 2: Occurrence of Fusarium toxins and their source organisms in durum wheat harvested in 2010. Journal of Agricultural and Food Chemistry, 61(23): 5438-5448
Ueda, A., Nishimoto, H., Kato, N., Hirano, T., Fukaya, M. (2007) Lineages and trichothecene mycotoxin types of Fusarium head blight pathogens of wheat and barley in Tokai district. Research Bulletin of the Aichi-ken Agricultural Research Center, 39: 17-23
Vatankhah, M., Saberi-Riseh, R., Moradzadeh, E.M., Afzali, H. (2019) Evaluation of some fungicides for the control of Fusarium dry rot of potato. Journal of Crop Protection, 8(3): 275-285,
Wegulo, S.N., Baenziger, P., Hernandez, N.J., Bockus, W.W., Hallen-Adams, H. (2015) Management of Fusarium head blight of wheat and barley. Crop Protection, 73: 100-107
White, T.J., Bruns, T.D., Lee, S.B., Taylor, J.W. (1990) Amplification and direct sequencing of fungal ribosomal rna genes for phylogenetics. in: Innis, M.A.; Gelfand, D.H.; Shinsky, J.J.; White, T.J. [ed.] PCR protocols: A guide to methods and applications, New York, USA: Academic Press, 315-322
Willyerd, K.T., Li, C., Madden, L.V., Bradley, C.A., Bergstrom, G.C., Sweets, L.E., McMullen, M., Ransom, J.K., Grybauskas, A., Osborne, L., Wegulo, S.N., Hershman, D.E., Wise, K., Bockus, W.W., Groth, D., Dill-Macky, R., Milus, E., Esker, P.D., Waxman, K.D., Adee, E.A. (2012) Efficacy and stability of integrating fungicide and cultivar resistance to manage Fusarium head blight and deoxynivalenol in wheat. Plant Disease, 96(7): 957-967
Wong, F.P., Midland, S.L. (2007) Sensitivity distributions of California populations of Colletotrichum cereale to the DMI fungicides propiconazole, myclobutanil, tebuconazole, and triadimefon. Plant Disease, 91(12): 1547-1555
Yang, C., Hamel, C., Vujanovic, V., Gan, Y. (2011) Fungicide: Modes of action and possible impact on nontarget microorganisms. ISRN Ecology, 2011: 1-8
Yin, Y., Liu, X., Li, B., Ma, Z. (2009) Characterization of sterol demethylation inhibitor-resistant isolates of Fusarium asiaticum and F. Graminearum collected from wheat in China. Phytopathology®, 99(5): 487-497
Zhang, H., van der Lee, T., Waalwijk, C., Chen, W.Q., Xu, J., Xu, J., Zhang, Y., Feng, J. (2012) Population analysis of the Fusarium graminearum species complex from wheat in China show a shift to more aggressive isolates. PLoS ONE, 7(2): e31722-e31722


article language: English
document type: Original Scientific Paper
DOI: 10.2298/PIF2202049I
received: 22/07/2022
accepted: 15/08/2022
published in SCIndeks: 09/09/2022
peer review method: double-blind
Creative Commons License 4.0

Related records

No related records