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Biotechnology in Animal Husbandry
2019, vol. 35, br. 3, str. 289-299
Određivanje genetičke strukture potočne pastrmke (Salmo trutta) iz ribnjaka "Braduljica", Srbija
Molerović Nikolaa ORCID, Rašković Božidarb ORCID, Đedović Radicab, Ostojić Andrić Dušicaa ORCID, Marković Zoranb, Marić Sašac ORCID
aInstitut za stočarstvo, Beograd-Zemun, Srbija
bUniverzitet u Beogradu, Poljoprivredni fakultet, Srbija
cUniverzitet u Beogradu, Biološki fakultet, Institut za zoologiju, Srbija

e-adresa: nikolamolerovic@gmail.com
Projekat:
Unapređenje proizvodnih kapaciteta šarana (Cyprinus carpio L) programima ishrane i selekcije (MPNTR - 31075)

Ključne reči: Salmo trutta; KR mtDNK; LDH nDNK; PCR; RFLP; selekcija
Sažetak
U cilju zaštite ţivotne sredine i genetiĉkih resursa potoĉne pastrmke (Salmo trutta) u radu je ispitivana genetiĉka struktura ove vrste na ribnjaku ―Braduljica‖ u blizini Ivanjice. DNK je izolovana iz odseĉaka peraja 10 jedinki, a nakon toga molekularne PCR-RFLP metode su korišćene za razlikovanje izmeĊu atlantske i dunavske linije na bazi kontrolnog regiona mitohondrijalne DNK (KR mtDNK) i jedarnog gena za laktat dehidrogenazu (LDH nDNK). Na osnovu fenotipskih karakteristika procenjeno je da je pet jedinki pripadalo alohtonoj atlantskoj liniji, a preostalih pet autohtonoj dunavskoj liniji potoĉne pastrmke. MeĊutim, rezultati molekularnih analiza pokazali su prisustvo visokog procenta alohtonih gena u analiziranom uzorku, što potvrĊuje hibridizaciju izmeĊu ove dve linije. TakoĊe, rezultati ukazuju na to da selekcija na osnovu fenotipa nije adekvatna. Kako bi ribnjak nastavio da se bavi gajenjem potoĉne pastrmke za potrebe poribljavanja okolnih reka, neophodno je da se eliminišu jedinke atlantske linije iz matiĉnog fonda.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/BAH1903289M
objavljen u SCIndeksu: 10.10.2019.
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