• citations in SCIndeks: [1]
  • citations in CrossRef:0
  • citations in Google Scholar:[]
  • visits in previous 30 days:10
  • full-text downloads in 30 days:3


article: 6 from 33  
Back back to result list
2018, vol. 33, iss. 3-4, pp. 197-204
Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides
Institute for Plant Protection and Environment, Belgrade, Serbia
Development of integrated approach in plant protection for control harmful organisms (MESTD - 31018)

Keywords: chitosan; antifungal activity; postharvest pathogens; apple fruits
Chitosan and its derivatives have been reported as a promising alternative for control of postharvest fungal pathogens. The objective of this study was to evaluate in vitro and in situ antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides isolated from decayed apple fruits. The fungi were tested in vitro using PDA medium with three concentrations of chitosan (1, 2 and 3 mg/ml). Fungal growth of the test pathogens was significantly affected by all chitosan doses (P<0.05) after 7 days of incubation at 25°C. Water solution of 3 mg/ml of chitosan inhibited completely the conidial germination of A. alternata and C. gloeosporioides after 18 h incubation at 25°C. The results obtained from biocontrol assay indicate that the inhibition of postharvest decay of A. alternata and C. gloeosporioides was significantly influenced by chitosan concentrations. Disease incidence in chitosan-treated fruit after 7 days incubation at 25°C was significantly lower than in the positive control for both fungi tested (P<0.05). A. alternata and C. gloeosporioides used in this study were progressively inhibited in vitro and in situ with increasing concentrations of chitosan from 1 to 3 mg/ml.
Aider, M. (2010) Chitosan application for active bio-based films production and potential in the food industry: Review. LWT - Food Science and Technology, 43(6): 837-842
Al-Hetar, M.Y., Zainal, A.M.A., Sariah, M., Wong, M.Y. (2010) Antifungal activity of chitosan against Fusarium oxysporum f. sp. cubense. Journal of Applied Polymer Science, 120(4): 2434-2439
Badawy, M.E.I., Rabea, E.I., Rogge, T.M., Stevens, C.V., Steurbaut, W., Höfte, M., Smagghe, G. (2005) Fungicidal and Insecticidal Activity of O-Acyl Chitosan Derivatives. Polymer Bulletin, 54(4-5): 279-289
Bautista-Banos, S., Hernandez-Lopez, M., Bosquez-Molina, E., Wilson, C.L. (2003) Effects of chitosan and plant extracts on growth of Colletotrichum gloeosporioides, anthracnose levels and quality of papaya fruit. Crop Protection, 22(9): 1087-1092
Benhamou, N., Thériault, G. (1992) Treatment with chitosan enhances resistance of tomato plants to the crown and root rot pathogen Fusarium oxysporum f. sp. radicis-lycopersici. Physiological and Molecular Plant Pathology, 41(1): 33-52
Choi, B., Jo, D., Anower, A.K., Mostafa, M., Islam, S., Sohn, S. (2016) Chitosan as an Immunomodulating Adjuvant on T-Cells and Antigen-Presenting Cells in Herpes Simplex Virus Type 1 Infection. Mediators of Inflammation, 2016: 1-12
Eckert, J., Ogawa, J.M. (1988) The Chemical Control Of Postharvest Diseases: Deciduous Fruits, Berries, Vegetables And Root/Tuber Crops. Annual Review of Phytopathology, 26(1): 433-469
Ghaouth, el A., Ponnampalam, R., Castaigne, F., Arul, J. (1992) Chitosan coating to extend the storage life of tomatoes. HortScience, 27(9): 1016-1018
Hassan, O., Chang, T. (2017) Chitosan for Eco-friendly Control of Plant Disease. Asian Journal of Plant Pathology, 11(2): 53-70
Hernández-Lauzardo, A.N., Velázquez-del, V.M.G., Veranza-Castelán, L., Melo-Giorgana, G.E., Guerra-Sánchez, M.G. (2010) Effect of chitosan on three isolates of Rhizopus stolonifer obtained from peach, papaya and tomato. Fruits, 65(4): 245-253
Holmes, G.J., Eckert, J.W. (1999) Sensitivity of Penicillium digitatum and P. italicum to Postharvest Citrus Fungicides in California. Phytopathology, 89(9): 716-721
Janisiewicz, W.J., Korsten, L. (2002) Biological control of postharvest diseases of fruits. Annual Review of Phytopathology, 40(1): 411-441
Jianglian, D., Shaoying, Z. (2013) Application of Chitosan Based Coating in Fruit and Vegetable Preservation: A Review. Journal of Food Processing and Technology, 04(05)
Jitareerat, P., Paumchai, S., Kanlayanarat, S., Sangchote, S. (2007) Effect of chitosan on ripening, enzymatic activity, and disease development in mango ( mangifera indica ) fruit. New Zealand Journal of Crop and Horticultural Science, 35(2): 211-218
Kauss, H., Waldmann, T., Jeblick, W., Euler, G., Ranjeva, R., Domard, A. (1989) Ca2+ is an Important but not the Only Signal in Callose Synthesis Induced by Chitosan, Saponins and Polyene Antibiotics. Berlin, Heidelberg: Springer Nature, 107-116
Kumar, P., Sethi, S., Sharma, R.R., Srivastav, M., Varghese, E. (2017) Effect of chitosan coating on postharvest life and quality of plum during storage at low temperature. Scientia Horticulturae, 226: 104-109
Lee, C.G., Koo, J.C., Park, J.K. (2016) Antifungal Effect of Chitosan as Ca 2+ Channel Blocker. Plant Pathology Journal, 32(3): 242-250
Liu, J., Tian, S., Meng, X., Xu, Y. (2007) Effects of chitosan on control of postharvest diseases and physiological responses of tomato fruit. Postharvest Biology and Technology, 44(3): 300-306
Manssouri, M., Znini, M., Harrak, A., Majid, L. (2016) Antifungal activity of essential oil from the fruits of Ammodaucus leucotrichus Coss. and Dur., in liquid and vapour phase against postharvest phytopathogenic fungi in apples. Journal of Applied Pharmaceutical Science, 131-136
Meng, X., Yang, L., Kennedy, J.F., Tian, S. (2010) Effects of chitosan and oligochitosan on growth of two fungal pathogens and physiological properties in pear fruit. Carbohydrate Polymers, 81(1): 70-75
Munhuweyi, K., Lennox, C.L., Meitz-Hopkins, J.C., Caleb, O.J., Sigge, G.O., Opara, U.L. (2016) In vitro effects of crab shell chitosan against mycelial growth of Botrytis sp. Penicillium sp. and Pilidiella granati. Acta Horticulturae, (1144): 403-408
Muñoz, Z., Moret, A., Garcés, S. (2009) Assessment of chitosan for inhibition of Colletotrichum sp. on tomatoes and grapes. Crop Protection, 28(1): 36-40
Notsu, S., Saito, N., Kosaki, H., Inui, H., Hirano, S. (1994) Stimulation of Phenylalanine Ammonia-lyase Activity and Lignification in Rice Callus Treated with Chitin, Chitosan, and Their Derivatives. Bioscience, Biotechnology, and Biochemistry, 58(3): 552-553
Oliveira, J.E.N., Gueddari, N.E., Moerschbacher, Bruno.M., Franco, T.T. (2012) Growth rate inhibition of phytopathogenic fungi by characterized chitosans. Brazilian Journal of Microbiology, 43(2): 800-809
Reddy, M., Arul, J., Ait-Barka, E., Angers, P., Richard, C., Castaigne, F. (1998) EVect of Chitosan on Growth and Toxin Production by Alternaria alternata f. sp. lycopersici. Biocontrol Science and Technology, 8(1): 33-43
Reglinski, T., Elmer, P.A.G., Taylor, J.T., Wood, P.N., Hoyte, S.M. (2010) Inhibition of Botrytis cinerea growth and suppression of botrytis bunch rot in grapes using chitosan. Plant Pathology, 59(5): 882-890
Rinaudo, M. (2006) Chitin and chitosan: Properties and applications. Progress in Polymer Science, 31(7): 603-632
Romanazzi, G., Nigro, F., Ippolito, A. (2001) Chitosan in the control of postharvest decay of some Mediterranean fruit. in: Muzzarelli R.A.A. [ed.] Chitin Enzymology, Italy: Atec, pp 141-146
Tayel, A.A., Moussa, S., El-Tras, W.F., Knittel, D., Opwis, K., Schollmeyer, E. (2010) Anticandidal action of fungal chitosan against Candida albicans. International Journal of Biological Macromolecules, 47(4): 454-457
Wang, L., Wu, H., Qin, G., Meng, X. (2013) Chitosan disrupts Penicillium expansum and controls postharvest blue mold of jujube fruit. Food Control, 41, 56-62
Wojdyła, A.T. (2004) Chitosan (biochikol 020 PC) in the control of some ornamental foliage diseases. Communications in Agricultural and Applied Biological Sciences, 69(4): 705-715
Xing, K., Zhu, X., Peng, X., Qin, S. (2015) Chitosan antimicrobial and eliciting properties for pest control in agriculture: A review. Agronomy for Sustainable Development, 35(2): 569-588
Zheng, F., Zheng, W., Li, L., Pan, S. (2017) Chitosan controls postharvest decay and elicits defense response in kiwifruit. Food Bioprocess Technology, 10.1007/s11947-017-1957-5


article language: English
document type: Original Scientific Paper
DOI: 10.2298/PIF1804197Z
published in SCIndeks: 10/01/2019
peer review method: double-blind
Creative Commons License 4.0

Related records

No related records