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2018, vol. 63, iss. 3, pp. 271-285
Antifungal activity of chitosan and its quaternized derivative in gel form and as an edible coating on cut cherry tomatoes
aFederal University of São Carlos, PPG Biotechnology, São Carlos, SP, Brazil
bUniversity of Ribeirão Preto, Bioscience Laboratory, Ribeirão Preto, SP, Brazil
cEmbrapa Instrumentation, National Nanotechnology Laboratory for Agriculture (LNNA), São Carlos, SP, Brazil
This research was supported by CNPq, Embrapa Rede Agro Nano and MCTI/SisNANO (National System of Laboratories in Nanotechnology)

Keywords: chitosan; antifungal activity; edible coatings; minimally processed tomatoes
The antifungal activities of medium molecular weight chitosan and its hydrosoluble derivative salt N,N,N-trimethylchitosan were examined as both gel and as a solid protective coating against three common food spoilage fungi (Penicilliumsp., wild Aspergillussp. and one standard strain of Aspergillusflavus). The salt derivative is characterized by having permanent positive charges and is expected to have a higher antimicrobial activity than commercial chitosan. In gel form, the minimum inhibitory concentration (MIC) resulted in the same value for both polymers against all tested fungi (> 2.0 gl-1). The derivative presented a significant fungistatic action against the Penicillium strain within the concentration range of 0.2 to 0.6 gl-1. When applied as protective coatings on freshly cut cherry tomatoes, the commercial chitosan appeared to be more effective in forming stable films and preventing fungal infestation than its derivative. Less than 20-25% of samples were infected after one week of incubation when compared to control (uncoated) and chitosan treated samples.
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article language: English
document type: Original Scientific Paper
DOI: 10.2298/JAS1803271S
published in SCIndeks: 02/11/2018
peer review method: double-blind
Creative Commons License 4.0

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