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Pesticidi i fitomedicina
2017, vol. 32, iss. 2, pp. 121-127
Antifungal activity of plant essential oils and selected Pseudomonas strains against Phomopsis theicola
Starović Miraa ORCID, Ristić Danijelaa ORCID, Aleksić Gorana, Pavlović Snežanab, Özcan Musa Mehmetc, Knežević Magdalenad ORCID, Jošić Draganad
aInstitute for Plant Protection and Environment, Belgrade, Serbia
bInstitute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade, Serbia
cDepartment of Food Engineering, Faculty of Agriculture, Selçuk University, Konya, Turkey
dInstitute of Soil Sciences, Belgrade, Serbia

email: algoran@sezampro.rs
Project:
Development of integrated approach in plant protection for control harmful organisms (MESTD - 31018)
New indigenous bacterial isolates Lysobacter and Pseudomonas as an important source of metabolites useful for biotechnology, plant growth stimulation and disease control: from isolates to inoculants (MESTD - 46007)

Keywords: Essential oils; Pseudomonas spp; PGPR; Phomopsis theicola; Antifungal activity
Abstract
Development of natural plant protection products as an alternative to synthetic fungicides is of significant importance regarding the environment. This study was carried out with an objective to investigate in vitro antifungal activities of several essential oils extracted from oregano, basil, myrtle and Turkish pickling herb, and the plant growth-promoting rhizobacteria in the genus Pseudomonas, against the phytopathogenic fungus Phomopsis theicola. Microdilution methods were used to determine the minimum inhibitory concentrations (MIC) of selected antimicrobial essential oils (EOs). All EOs exhibited significant levels of antifungal activity against the tested fungal isolates. The oregano EO was found the most potent one (MIC - 5.5 µg/mL), followed by basil (MIC - 75.0µg/mL), myrtle (MIC - 775 µg/mL) and Turkish pickling herb (MIC - 7750 µg/mL). Inhibition of Ph. theicola mycelial growth was observed for all tested Pseudomonas spp. strains. K113 and L1 strains were highly effective and achieved more than 60% of fungal growth inhibition using the overnight culture and more than 57% inhibition by applying cell-free supernatants of both strains. A future field trial with K113 and L1 cultures and cell-free supernatants, containing extracellular metabolites toward Ph. theicola, will estimate their effectiveness and applicability as an alternative to chemical protection of apple trees.
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article language: English
document type: Original Scientific Paper
DOI: 10.2298/PIF1702121S
published in SCIndeks: 05/10/2017
peer review method: double-blind
Creative Commons License 4.0

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