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2018, vol. 33, iss. 1, pp. 19-26
Antifungal activity of cinnamon and clove essential oils against button mushroom pathogens Cladobotryum dendroides (Bull.) W. Gams & Hooz and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk
aUniversity of Belgrade, Faculty of Agriculture, Serbia
bInstitute of Pesticides and Environmental Protection, Belgrade, Serbia
Studies on plant pathogens, arthropods, weeds, and pesticides with a view to developing the methods of biorational plant protection and safe food production (MESTD - 31043)
Development of integrated management of harmful organisms in plant production in order to overcome resistance and to improve food quality and safety (MESTD - 46008)

Antifungal activity of two essential oils, cinnamon (Cinnamomum verum J. Presl) and clove (Syzygium aromaticum (L.) Merrill & Perry), was evaluated against Cladobotryum dendroides (Bull.) W. Gams & Hooz, and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk, the causal agents of cobweb and dry bubble disease of cultivated mushroom. Inhibitory and fungicidal activity of the selected essential oils was assayed using three methods: microdilution, macrodilution fumigant and macrodilution contact method. Comparing all three methods, clove essential oil showed stronger activity than cinnamon against both fungi, having minimum inhibitory concentration (MIC) at the lowest concentrations tested (1.56, 0.02 and 0.1 µl ml-1, respectively). However, cinnamon oil was more toxic to L. fungicola var. fungicola then to C. dendroides in all three methods. Both oils exhibited stronger antifungal effects when used in the macrodilution fumigant than in contact method. The results showed that both cinnamon and clove essential oils have the potential for further in vivo experiments against L. fungicola var. fungicola and C. dendroides and indicated a possible use of these oils in integrated disease management in mushrooms.
Angelini, P., Pagiotti, R., Granetti, B. (2008) Effect of antimicrobial activity of Melaleuca alternifolia essential oil on antagonistic potential of Pleurotus species against Trichoderma harzianum in dual culture. World Journal of Microbiology and Biotechnology, 24(2): 197-202
Beyer, D.M., Kremser, J.J. (2004) Evaluation of fungicide tolerance and control for three fungal diseases of mushrooms. in: Romaine C.P., Keil C.B., Rinker D.L., Royse D. J. [ed.] Science and Cultivation of Edible and Medicinal Fungi, Mushroom Science XVI., University Park, USA: Penn State University, str. 521-529
Bugarski, D., Gvozdenović, Đ. (1998) Neophodni uslovi za intenzivnu proizvodnju gljiva. Zbornik radova Instituta za ratarstvo i povrtarstvo, 30, str. 191-194
Byron, E.M., Hall, A.M. (2002) Inhibition of common cereal fungi by clove oil and eucalyptus oil. in: Proceedings of the BCPC Conference - Pests & Diseases, Brighton, 765-768
Đurović-Pejčev, R., Potočnik, I., Milijašević-Marčić, S., Todorović, B., Rekanović, E., Stepanović, M. (2014) Antifungal activity of six plant essential oils from Serbia against Trichoderma aggressivum f. europaeum. Pesticidi i fitomedicina, vol. 29, br. 4, str. 291-297
Gea, F.J., Tello, J.C., Navarro, M.J. (2003) Occurrence of Verticillium fungicola var. fungicola on Agaricus bitorquis Mushroom Crops in Spain. Journal of Phytopathology, 151(2): 98-100
Grogan, H.M. (2008) Challenges facing mushroom disease control in the 21st century. in: Mushroom Biology and Mushroom Products, Sixth International Conference, Bonn, Germany, Proceeding, str. 120-127
Grogan, H.M., Keeling, C., Jukes, A.A. (2000) In vivo response of the mushroom pathogen Verticillium fungicola (dry bubble) to prochloraz - manganeze. in: Proc. of the BCPC Conference - Pests & Diseases, Brighton, UK, str. 273-278
Grogan, H.M., Gaze, R.H. (2000) Fungicide resistance among Cladobotryum spp.: Causal agents of cobweb disease of the edible. Mycological Research, 104(3): 357-364
Kaiser, C., van der Merwe, R., Bekker, T.F., Labuschagne, N. (2005) In vitro inhibition of mycelial growth of several phytopathogenic fungi, incuding Phytophthora cinnamomi by soluble silicon. South African Avocado Growers' Association Yearbook, 28, 70-74
Kosanović, D., Potočnik, I., Duduk, B., Vukojević, J., Stajić, M., Rekanović, E., Milijašević-Marčić, S. (2013) Trichoderma species on Agaricus bisporus farms in Serbia and their biocontrol. Annals of Applied Biology, 163(2): 218-230
Kosanović, D., Potočnik, I., Vukojević, J., Stajić, M., Rekanović, E., Stepanović, M., Todorović, B. (2015) Fungicide sensitivity of Trichoderma spp. from Agaricus bisporus farms in Serbia. Journal of Environmental Science and Health, Part B, 50(8): 607-613
Mckay, G.J., Egan, D., Morris, E., Scott, C., Brown, A.E. (1999) Genetic and morphological characterization of Cladobotryum species causing cobweb disease of mushrooms. Appl Environ Microbiol, 65(2): 606-10
Orwa, C., Jamnadass, R.H., Kindt, R., Mutua, A., Simons, A. (2009) Agroforestry database: A tree reference and selection guide version 4.O. Retrieved from
Potočnik, I., Tanović, B., Milijašević, S., Rekanović, E., Todorović, B. (2005) Response of the mushroom pathogen Verticillium fungicola (Preuss) Hasebrauk (dry bubble) to some essential oils. Rev. Cytol. Biol. Vegetal. - Le Botaniste, 28 (s.i): 388-392
Potočnik, I., Vukojević, J., Stajić, M., Rekanović, E., Milijašević, S., Todorović, B., Stepanović, M. (2009) In vitro toxicity of selected fungicides from the groups of benzimidazoles and demethylation inhibitors to Cladobotryum dendroides and Agaricus bisporus. Journal of Environmental Science and Health, Part B, 44(4): 365-370
Potočnik, I., Vukojević, J.B., Stajić, M.M., Rekanović, E., Stepanović, M., Milijašević, S., Todorović, B. (2010) Toxicity of biofungicide Timorex 66 EC to Cladobotryum dendroides and Agaricus bisporus. Crop Protection, vol. 29, br. 3, str. 290-294
Potočnik, I., Vukojević, J.B., Stajić, M.M., Tanović, B., Rekanović, E. (2010) Sensitivity of Mycogone perniciosa, Pathogen of Culinary-Medicinal Button Mushroom Agaricus bisporus (J. Lge) Imbach (Agaricomycetideae), to Selected Fungicides and Essential Oils. International Journal of Medicinal Mushrooms, vol. 12, br. 1, str. 91-98
Potočnik, I., Stepanović, M., Rekanović, E., Todorović, B., Milijašević-Marčić, S. (2015) Disease control by chemical and biological fungicides in cultivated mushrooms: Button mushroom, oyster mushroom and shiitake. Pesticidi i fitomedicina, vol. 30, br. 4, str. 201-208
Potočnik, I., Vukojević, J., Stajić, M., Tanović, B., Todorović, B. (2008) Fungicide sensitivity of selected Verticillium fungicola isolates from Agaricus bisporus farms. Archives of Biological Sciences, vol. 60, br. 1, str. 151-157
Putievsky, E., Ravid, U., Dudai, N. (1986) The Essential Oil and Yield Components from Various Plant Parts of Salvia fruticosa. Journal of Natural Products, 49(6): 1015-1017
Regnier, T., Combrinck, S. (2010) In vitro and in vivo screening of essential oils for the control of wet bubble disease of Agaricus bisporus. South African Journal of Botany, 76(4): 681-685
Sokovic, M.D., Vukojevic, J.B., Marin, P.D., Brkic, D.D., Vajs, V.E., van Griensven, L. (2009) Chemical Composition of Essential Oils of Thymus and Mentha Species and Their Antifungal Activities. Molecules, vol. 14, br. 1, str. 238-249
Soković, M., Griensven, L.J.L.D. (2006) Antimicrobial activity of essential oils and their components against the three major pathogens of the cultivated button mushroom, Agaricus bisporus. European Journal of Plant Pathology, 116(3): 211-224
Suhr, K.I., Nielsen, P.V. (2003) Antifungal activity of essential oils evaluated by two different application techniques against rye bread spoilage fungi. Journal of Applied Microbiology, 94(4): 665-674
Tanović, B., Milijašević, S., Obradović, A. (2007) In vitro effect of plant essential oils on growth of some soil-borne pathogens. Acta Horticulturae, 729, 483-487
Tanović, B., Obradovic, A., Potocnik, I. (2004) Effects of thyme essential oil on Pythium sp. in: Proc. of ESNA XXXIV Annual Meetining, Novi Sad, Serbia and Montenegro, str. 502-505, b
Tanović, B., Potočnik, I., Delibašić, G., Ristić, M., Kostić, M., Marković, M. (2009) In vitro effect of essential oils from aromatic and medicinal plants on mushroom pathogens: Verticillium fungicola var. fungicola, Mycogone perniciosa, and Cladobotryum sp. Archives of Biological Sciences, vol. 61, br. 2, str. 231-237
Tanović, B., Potočnik, I., Stanisavljević, B., Đorđević, M., Rekanović, E. (2006) Response of Verticillium fungicola var. fungicola, Mycogone perniciosa and Cladobotryum sp. Mushroom pathogens to some essential oils. Pesticidi i fitomedicina, vol. 21, br. 3, str. 231-237
Wilson, C.L., Solar, J.M., el Ghaouth, A., Wisniewski, M.E. (1997) Rapid evaluation of plant extracts and essential oils for antifungal activity against Botrytis cinerea. Plant Disease, 81(2): 204-210


article language: English
document type: Original Scientific Paper
DOI: 10.2298/PIF1801019L
published in SCIndeks: 11/05/2018
peer review method: double-blind
Creative Commons License 4.0

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