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2021, vol. 71, br. 3, str. 197-206
Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka
aUniverzitet u Beogradu, Farmaceutski fakultet
bUniverzitet u Beogradu, Institut za primenu nuklearne energije - INEP
cUniverzitet u Beogradu, Institut za nuklearne nauke Vinča, Beograd-Vinča

e-adresabiljana.potparevic@pharmacy.bg.ac.rs
Projekat:
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Univerzitet u Beogradu, Farmaceutski fakultet) (MPNTR - 451-03-68/2020-14/200161)

Ključne reči: oštećenje DNK; komet test; jagoda (cv. Romina); antocijanini; leukociti
Sažetak
Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/arhfarm71-31060
objavljen u SCIndeksu: 12.06.2021.
metod recenzije: jednostruko anoniman
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