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2021, vol. 71, iss. 3, pp. 197-206
Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes
aUniversity of Belgrade, Faculty of Pharmacy
bUniversity of Belgrade, Institute for the Application of Nuclear Energy - INEP
cUniversity of Belgrade, Institute of Nuclear Sciences 'Vinča', Belgrade-Vinča
Ministry of Education, Science and Technological Development, Republic of Serbia (Institution: University of Belgrade, Faculty of Pharmacy) (MESTD - 451-03-68/2020-14/200161)

Keywords: DNA damage; comet assay; strawberry (cv. Romina); anthocyanins; leukocytes
Strawberry fruit Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), rich in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxide in vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 µg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposure of the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the posttreatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 µg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent.
Alvarez-Suarez, J.M., Dekanski, D., Ristić, S., Radonjić, N.V., Petronijević, N.D., Giampieri, F., Astolfi, P., González-Paramás, A.M., Santos-Buelga, C., Tulipani, S., Quiles, J.L., Mezzetti, B., Battino, M. (2011) Strawberry Polyphenols Attenuate Ethanol-Induced Gastric Lesions in Rats by Activation of Antioxidant Enzymes and Attenuation of MDA Increase. PLoS One, 6(10): e25878
Anderson, D., Yu, T., Phillips, B., Schmezer, P. (1994) The effect of various antioxidants and other modifying agents on oxygen-radical-generated DNA damage in human lymphocytes in the COMET assay. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 307(1): 261-271
Capocasa, F., Balducci, F., di Vittori, L., Mazzoni, L., Stewart, D., Williams, S., et al. (2016) Romina and Cristina: Two New Strawberry Cultivars with High Sensorial and Nutritional Values. International Journal of Fruit Science, 16(sup1): 207-219
Castaneda-Ovando, A., Pacheco-Hernandez, M.D.L., Paez-Hernandez, M.E., Rodriguez, J.A., Galan-Vidal, C.A. (2009) Chemical studies of anthocyanins: A review. Food Chemistry, 113 (4): 859-871
Collins, A., Koppen, G., Valdiglesias, V., Dusinska, M., Kruszewski, M., Møller, P., et al. (2014) The comet assay as a tool for human biomonitoring studies: The ComNet Project. Mutation research. Reviews in mutation research, 759: 27-39
Curtis, P.J., Kroon, P.A., Hollands, W.J., Walls, R., Jenkins, G., Kay, C.D., et al. (2009) Cardiovascular Disease Risk Biomarkers and Liver and Kidney Function Are Not Altered in Postmenopausal Women after Ingesting an Elderberry Extract Rich in Anthocyanins for 12 Weeks. Journal of Nutrition, 139(12): 2266-71
da Silva, F.L., Escribano-Bailon, M.T., Perez, A.J.J., Rivas-Gonzalo, J.C., Santos-Buelga, C. (2007) Anthocyanin pigments in strawberry. LWT - Food Science and Technology, 40(2): 374-382
de Pascual-Teresa, S., Moreno, D.A., García-Viguera, C. (2010) Flavanols and Anthocyanins in Cardiovascular Health: A Review of Current Evidence. International Journal of Molecular Sciences, 11(4): 1679-1703
Forbes-Hernández, T.Y., Gasparrini, M., Afrin, S., Cianciosi, D., González-Paramás, A.M., Santos-Buelga, C., Mezzetti, B., Quiles, J., Battino, M., Giampieri, F., Bompadre, S. (2017) Strawberry (cv. Romina) Methanolic Extract and Anthocyanin-Enriched Fraction Improve Lipid Profile and Antioxidant Status in HepG2 Cells. International Journal of Molecular Sciences, 18(6): 1149
Giampieri, F., Alvarez-Suarez, J.M., Mazzoni, L., Forbes-Hernandez, T.Y., Gasparrini, M., Gonzàlez-Paramàs, A.M., Santos-Buelga, C., Quiles, J.L., Bompadre, S., Mezzetti, B., Battino, M. (2014) An anthocyanin-rich strawberry extract protects against oxidative stress damage and improves mitochondrial functionality in human dermal fibroblasts exposed to an oxidizing agent. Food & Function, 5(8): 1939-48
He, J., Giusti, M.M. (2011) High-purity isolation of anthocyanins mixtures from fruits and vegetables: A novel solid-phase extraction method using mixed mode cation-exchange chromatography. Journal of Chromatography A, 1218(44): 7914-7922
Horbowicz, M., Kosson, R., Grzesiuk, A., Dębski, H. (2008) Anthocyanins of Fruits and Vegetables: Their Occurrence, Analysis and Role in Human Nutrition. Journal of Fruit and Ornamental Plant Research, 68(1): 5-22
Hrelia, S., Angeloni, C. (2020) New Mechanisms of Action of Natural Antioxidants in Health and Disease. Antioxidants, Basel, Switzerland, 9(4): 344
Konczak, I., Zhang, W. (2004) Anthocyanins: More Than Nature's Colours. Journal of Biomedicine and Biotechnology, 2004(5): 239-240
López-Romero, D., Izquierdo-Vega, J.A., Morales-González, J.A., Madrigal-Bujaidar, E., Chamorro-Cevallos, G., Sánchez-Gutiérrez, M., et al. (2018) Evidence of Some Natural Products with Antigenotoxic Effects. Part 2: Plants, Vegetables, and Natural Resin. Nutrients, 10(12):1954
Qin, Y., Xia, M., Ma, J., Hao, Y., Liu, J., Mou, H., et al. (2009) Anthocyanin supplementation improves serum LDLand HDL-cholesterol concentrations associated with the inhibition of cholesteryl ester transfer protein in dyslipidemic subjects. American Journal of Clinical Nutrition, 90(3): 485-492
Rice-Evans, C.A., Miller, N.J. (1996) Antioxidant activities of flavonoids as bioactive components of food. Biochem Soc Trans, 24(3): 790-5
Rice-Evans, C.A., Miller, N.J., Paganga, G. (1997) Antioxidant properties of phenolic compounds. Trends in Plant Science, 2(4): 152
Singh, N.P., McCoy, M.T., Tice, R.R., Schneider, E.L. (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res, 175(1): 184-91
Skrovankova, S., Sumczynski, D., Mlcek, J., Jurikova, T., Sochor, J. (2015) Bioactive Compounds and Antioxidant Activity in Different Types of Berries. International Journal of Molecular Sciences, 16(10): 24673-24706
Ştefănuţ, M.N., Căta, A., Pop, R., Tănasie, C., Boc, D., Ienaşcu, I., et al. (2013) Anti-hyperglycemic Effect of Bilberry, Blackberry and Mulberry Ultrasonic Extracts on Diabetic Rats. Plant Foods for Human Nutrition, 68(4): 378-384
Tulipani, S., Mezzetti, B., Capocasa, F., Bompadre, S., Beekwilder, J., de Vos, R.C.H., Capanoglu, E., Bovy, A., Battino, M. (2008) Antioxidants, Phenolic Compounds, and Nutritional Quality of Different Strawberry Genotypes. Journal of Agricultural and Food Chemistry, 56(3): 696-704


article language: English
document type: Original Scientific Paper
DOI: 10.5937/arhfarm71-31060
published in SCIndeks: 12/06/2021
peer review method: single-blind
Creative Commons License 4.0

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