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2015, vol. 69, iss. 5, pp. 511-522
Effects of pH on the stability of cyanidin and cyanidin 3-O-β-glucopyranoside in aqueous solution
aCollege of Agriculture and Food Technology, Prokuplje
bDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
cUniveristy of Niš, Faculty of Science, Department of Chemistry
dUniveristy of Niš, Faculty of Medicine
eDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia + Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CipKeBiP), Ljubljana, Slovenia

emailvioletachem@gmail.com
Project:
Slovenian Research Agency through the P4-0121 Research Programme and the Bilateral Project between the Republic of Slovenia and the Republic of Serbia BI-RS/12-13-015. V

Abstract
The colour variation, colour intensity and stability at various pH values (2.0, 4.0, 7.0 and 9.0) of cyanidin 3-O-β-glucopyranoside (Cy3Glc) and its aglycone cyanidin were investigated during a period of 8 hours storage at 25°C. Our data showed that pH of aqueous solution had impact on spectroscopic profile of cyanidin and Cy3Glc. Beginning with the most acidic solutions, increasing the pH induce bathochromic shifts of absorbance maximum in the visible range for all examined pH values (with the exception pH 4.0 for cyanidin), while the presence of the 3-glucosidic substitution induce hypsochromic shift. Compared to cyanidin, Cy3Glc has higher colour intensity and higher stability in the whole pH range, except at pH 7.0. The 3-glucosidic substitution influences on the colour intensity of Cy3Glc in the alkaline region. After 8-hour incubation of Cy3Glc and cyanidin at pH 2.0 and 25°C, 99% of Cy3Glc and only 27% of cyanidin remained unchanged.

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article language: English
document type: Scientific Paper
DOI: 10.2298/HEMIND140711072R
published in SCIndeks: 18/03/2016

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