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2015, vol. 69, iss. 2, pp. 155-163
Investigation of fluorescence properties of cyanidin and cyanidin 3-O-(-glucopyranoside
aVisoka poljoprivredno-prehrambena škola strukovnih studija, Prokuplje
bDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia
cUniveristy of Niš, Faculty of Science, Department of Chemistry
dUniveristy of Niš, Faculty of Medicine
eDepartment of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia + Centre of Excellence for Integrated Approaches in Chemistry and Biology of Proteins (CipKeBiP), Ljubljana, Slovenia

emailvioletachem@gmail.com
Project:
Slovenian Research Agency through the P4-0121 Research Programme and the Bilateral Project between the Republic of Slovenia and the Republic of Serbia BI-RS/12-13-015. V.R. was partly financed by a CEEPUS SI-8402/2010

Abstract
The absorption and fluorescence emission spectra of cyanidin and cyanidin 3-O-(-glucopyranoside (Cy3Glc) at pH 5.5 in aqueous solution have been studied. The most effective fluorescence excitations of cyanidin were at the UV absorption maxima at 220 and 230 nm and at higher wavelengths at 270 and 280 nm. Cyanidin exhibits fluorescence emission maxima at 310 nm and in the visible range at 615 nm. The most effective fluorescence excitation for the Cy3Glc was at 220 and at 230 nm, and at higher wavelengths at 300 and at 310 nm. The Cy3Glc has fluorescence emission spectra with the maximum at 380 nm and does not show fluorescence emission in the visible range. If compare fluorescence emission spectra of cyanidin and Cy3Glc, it can be seen that fluorescence emission intensity for cyanidin is significantly higher than that for Cy3Glc. These results revealed the impact of 3-glucosidic substitution at C-3 of aglycone (to form Cy3Glc) on the significantly decrease in fluorescence emission intensity, and disappearance of the fluorescence emission in visible wavelength range.

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article language: English
document type: Scientific Paper
DOI: 10.2298/HEMIND140203030R
published in SCIndeks: 13/01/2016

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