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2012, vol. 63, br. 3, str. 130-138
Identifikacija Colletotrichum acutatum sa ploda nektarine
Institut za zaštitu bilja i životnu sredinu, Beograd, Srbija

e-adresazivkovicsvetla@gmail.com
Projekat:
Razrada integrisanog upravljanja i primene savremenih principa suzbijanja štetnih organizama u zaštiti bilja (MPNTR - 31018)

Sažetak
Izolati Colletotrichum spp. dobijeni 2010. godine, sa antraknoznih plodova nektarine identifikovani su pomoću morfoloških i molekularnih metoda. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu nektarine, 7 dana nakon inokulacije. Na PDA podlozi izolati formiraju tamno zelenu do tamno sivu miceliju. Stopa rasta izolata sa nektarine i referentnog soja C.acutatum je bila niža u odnosu na referentni soj C.gloeosporioides. Konidije su hialinske, neseptirane i fusiformne. Apresorije su tamno braon boje, glatke, jednostavne, okruglastog ili oblika izdužene palice. Korišćenjem para prajmera CaInt2/ITS4 iz genoma DNA izolata sa ploda nektarine i referentnog soja C. acutatum - CBS 294.67, amplifikovan je fragment veličine 490 bp. Na osnovu ovih rezultata, u Srbiji je kao prouzrokovač antrakoze plodova nektarine identifikovana vrsta C.acutatum.
Reference
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jezik rada: engleski
vrsta rada: naučni članak
objavljen u SCIndeksu: 22.03.2013.

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