Metrika

  • citati u SCIndeksu: [1]
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[]
  • posete u poslednjih 30 dana:3
  • preuzimanja u poslednjih 30 dana:3

Sadržaj

članak: 8 od 13  
Back povratak na rezultate
2010, vol. 25, br. 3, str. 231-239
Morfološka i molekularna identifikacija Colletotrichum acutatum sa ploda paradajza
aInstitut za zaštitu bilja i životnu sredinu, Beograd, Srbija
bUniverzitet u Novom Sadu, Poljoprivredni fakultet, Srbija

e-adresazsvetlana@sezampro.rs
Sažetak
Četiri glavna prouzrokovača antraknoze paradajza iz roda Colletotrichum su: Colletotrichum gloeosporioides, Colletotrichum acutatum, Colletotrichum coccodes i Colletotrichum dematium. U Srbiji je tokom poslednjih godina zabeležena pojava antraknoze ploda paradajza. Tipični simptomi na plodu su tamne, ulegnute, kružne lezije sa masom narandžastih konidija. Sa obolelih plodova paradajza dobijeni su izolati patogena koji na KDA podlozi obrazuju bele do sivo obojene kolonije. Acervuli crne boje formiraju se u kulturi oko centra kolonije. Konidije su hialinske, neseptirane, fusiformne ili ređe cilindrične. Apresorije su glatke, jednostavne, okruglastog ili oblika izdužene palice, svetlo do tamno braon boje. Test patogenosti je obavljen sa reprezentativnim izolatima, na odabranim, zdravim plodovima. Svi ispitivani izolati prouzrokuju antraknozne lezije na plodu paradajza 7 dana nakon inokulacije. Kohovi postulati su zadovoljeni reizolacijama sa inokulisanih plodova paradajza. PCR analiza (korišćenjem para prajmera specifičinog za vrstu, CaInt2/ITS4) iz genoma DNA izolata sa paradajza rezultirala je amplifikacijom produkta od 490 bp, specifičnog za C. acutatum, što je potvrdilo identitet patogena. Na osnovu morfoloških i molekularnih karakteristika izolati sa ploda paradajza determinisani su kao C. acutatum.
Reference
Arsenijević, M. (1984) Glomerella cingulata (Stan) Spaul et Schrenk: Conidial phase of Colletotrichum gloeosporioides (Penz) Sacc. as a parasite of sour cherry fruits. Fruit Growing in Yugoslavia, 18, str. 45-49
Bailey, J.A., OConnell, R.J., Pring, R.J., Nash, C. (1992) Infection strategies of colletotrichum species. u: Bailey J.A., Jeger M.J. [ur.] Colletotrichum: Biology, Pathology and Control, Wallingford, UK: CAB Int, str. 88-120
Baxter, A.P., Westhuizen, G.C.A., vander Eicker, A. (1983) Morphology and taxonomy of South African isolates of Colletotrichum. South African J. Bot, 2, str. 259-289
Brown, A.E., Sreenivasaprasad, S., Timmer, L.W. (1996) Molecular characterization of slow-growing orange and Key lime anthracnose strains of Colletotrichum from citrus as C. acutatum. Phytopathology, 86(5): 523
Byrne, J.M., Hausbeck, M.K., Hammerschmidt, R. (1997) Conidial germination and appressorium formation of Colletotrichum coccodes on tomato foliage. Plant Disease, 81(7): 715-718
Dillard, H.R. (1989) Effect of temperature, wetness duration, and inoculums density on infection and lesion development of Colletotrichum coccodes on tomato fruit. Phytopathology, 79(10): 1063
Dyko, B.J., Mordue, J.E.M. (1979) Colletotrichum acutatum. u: CMI descriptions of pathogenic fungi and bacteria, Kew, Surrey, England: Commonwealth Mycological Institute, br. 630
Förster, H., Adaskaveg, J.E. (1999) Identification of Subpopulations of Colletotrichum acutatum and Epidemiology of Almond Anthracnose in California. Phytopathology, 89(11): 1056-65
Freeman, S., Katan, T., Shabi, E. (1998) Characterization of Colletotrichum species responsible for anthracnose disease of various fruits. Plant Disease, vol. 82 (6): 596-605
Freeman, S., Minz, D., Jurkevitch, E., Maymon, M., Shabi, E. (2000) Molecular analyses of colletotrichum species from almond and other fruits. Phytopathology, 90(6): 608-14
Freeman, S., Minz, D., Maymon, M., Zveibil, A. (2001) Genetic diversity within Colletotrichum acutatum sensu Simmonds. Phytopathology, 91(6): 586-592
Gunnell, P.S., Gubler, W. (1992) Taxonomy and Morphology of Colletotrichum Species Pathogenic to Strawberry. Mycologia, 84(2): 157
Ivanović, M., Ivanović, D. (1992) Study of Colletotrichum gloeosporioides, Causal agent of anthracnose on sour cherry and effects of some fungicides on fungi in vitro. Plant Protection, 201, str. 211-218
Ivanović, M.S., Duduk, B.B., Ivanović, M.M., Ivanović, M.S. (2007) Antraknoza - nova bolest jagode u Srbiji i njena kontrola fungicidima. Zbornik Matice srpske za prirodne nauke, br. 113, str. 71-81
Jelev, Z.J., Bobev, S.G., Minz, D., Maymon, M., Freeman, S. (2008) First report of anthracnose fruit rot caused by Colletotrichum acutatum on pepper and tomato in Bulgaria. Plant Disease, 92, 172
Johnston, P.R., Jones, D. (1997) Relationships among Colletotrichum Isolates from Fruit-Rots Assessed Using rDNA Sequences. Mycologia, 89(3): 420
Lardner, R., Johnston, P.R., Plummer, K.M., Pearson, M.N. (1999) Morphological and molecular analysis of Colletotrichum acutatum sensu lato. Mycological Research, 103(3): 275-285
LeBoeuf, J. (2007) Identifying the species causing anthracnose of pepper and tomato in Southern Ontario. OMAFRA, http://www.omafra.gov.on.ca/crops/html
Martin, M.P., Garcia-Figueres, F. (1999) Colletotrichum acutatum and C. gloeosporioides caused anthracnose on olives. European Journal of Plant Pathology, 105(8): 733-741
Panjan, M., Lušin, V. (1963) Diseases of greenhouse-grown tomato. Plant Protection, 75, str. 509-516
Sanders, G.M., Korsten, L. (2003) A comparative morphological study of South African avocado and mango isolates of Colletotrichum gloeosporioides. Canadian Journal of Botany, 81(8): 877-885
Sanogo, S., Pennypacker, S.P., Stevenson, R.E., Macnab, A.A. (1997) Weather variables associated with infection of tomato fruit by Colletotrichum coccodes. Plant Disease, 81(7): 753-756
Smith, B.J., Black, L.L. (1990) Morphological, cultural, and pathogenic variation among Colletotrichum species isolated from strawberry. Plant Dis, 74, str. 69-76
Sreenivasaprasad, S., Sharada, K., Brown, A.E., Mills, P.R. (1996) PCR-based detection of Colletotrichum acutatum on strawberry. Plant Pathol, 45: 650-655
Sutton, B.C. (1992) The genus Glomerella and its anamorph Colletotrichum. u: Bailey J. A., Jeger M. J. [ur.] Colletotnchum, biology, pathology and control, Melksham: CAB International - Redwood Press, p 1-24
Than, P.P., Jeewon, R., Hyde, K.D., Pongsupasamit, S., Mongkolporn, O., Taylor, P.W.J. (2008) Characterization and pathogenicity of Colletotrichum species associated with anthracnose on chilli (Capsicum spp.) in Thailand. Plant Pathology, 57(3): 562-572
Trkulja, V. (2003) Pathogenic, morphological and growing properties of Colletotrichum spp. causal agent of Glomerella cingulata on apple fruits. Belgrade: Faculty of Agriculture, Doctoral thesis
Whitelaw-Weckert, M.A., Curtin, S.J., Huang, R., Steel, C.C., Blanchard, C.L., Roffey, P.E. (2007) Phylogenetic relationships and pathogenicity of Colletotrichum acutatum isolates from grape in subtropical Australia. Plant Pathology, 56(3): 448-463
Živković, S., Stojanović, S., Ivanović, Ž., Gavrilović, V., Balaž, J. (2008) Colletotrichum acutatum: Causal agent of tomato anthracnose. u: Conference on Plant Protection, (IX), Zlatibor, Book of Abstracts, pp. 85-86
Živković, S., Stojanović, S., Gavrilović, V., Ivanović, Ž., Balaž, J. (2009) Morphological and molecular analysis of Colletotrichum species from pear. u: Congress of the Serbian Genetic Society, (IV), Tara, Book of Abstracts, pp. 251
 

O članku

jezik rada: engleski
vrsta rada: naučni članak
DOI: 10.2298/PIF1003231Z
objavljen u SCIndeksu: 18.01.2011.

Povezani članci

Nema povezanih članaka