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2020, vol. 24, br. 2, str. 72-76
In vitro potencijal antagonista Bacillus spp. za suzbijanje fitopatogena Xanthomonas euvesicatoria
aUniverzitet u Novom Sadu, Tehnološki fakultet
bUniverzitet u Novom Sadu, Poljoprivredni fakultet
cNaučni institut za ratarstvo i povrtarstvo, Novi Sad

e-adresapaj@tf.uns.ac.rs
Projekat:
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Univerzitet u Novom Sadu, Tehnološki fakultet) (MPNTR - 451-03-68/2020-14/200134)
This research was supported by the Autonomous Province of Vojvodina - Provincial Secretariat for Higher Education and Scientific Research within the framework of the Short-term project of special interest for sustainable development in the AP Vojvodina entitled "Implementation of circular economy principles in technology of production of biocontrol agents" (project 142-451-3243/2020-03).

Ključne reči: antimikrobna aktivnost; paprika; bakteriozna pegavost; biomasa; antimikrobna jedinjenja; osetljivost
Sažetak
Bakterijski patogen Xanthomonas euvesicatoria predstavlja glavnog uzročnika bakteriozne pegavosti, stoga predstavlja značajan problem u poljoprivrednoj praksi usled izazivanja velikih gubitaka prinosa u proizvodnji paprike i paradajza. Uobičajena sredstva za suzbijanje i kontrolu ovog fitopatogena predstavljaju preparati na bazi bakra. Međutim, razvoj rezistentnosti prema pesticidima na bazi bakra doveo je do zaokreta u polju suzbijanja ovih patogena ka primeni biopesticida, odnosno bioloških kontrolnih agensa. Bakterije roda Bacillus i njihovi metaboliti sa izraženom antimikrobnom aktivnošću protiv ciljanih fitopatogena predstavljaju najperspektivnije aktivne komponente biokontrolnih preparata za zaštitu bilja. U ovom istraživanju nekoliko sojeva roda Bacillus ispitano je sa ciljem odabira antagonista dovoljno efikasnih u suzbijanju sojeva Xanthomonas euvesicatoria, koji su izolovani sa listova paprike sa simptomima bakteriozne pegavosti. Prilikom testiranja antimikrobne aktivnosti uzoraka kultivacionih tečnosti, koji sadrže i biomasu testiranih antagonista, najbolji rezultati u suzbijanju testiranih fitopatogena su postignuti primenom izolata Bacillus sp3. Sa druge strane, prilikom testiranja antimikrobne aktivnosti supernatanata oslobođenih biomase antagonista, koji sadrže samo produkovana antimikrobna jedinjenja, izolati Bacillus sp1 i Bacillus sp2 su pokazali najveću antimikrobnu aktivnost protiv fitopatogena Xanthomonas euvesicatoria. Rezultati ovog istraživanja predstavljaju osnovu za dalji razvoj bioprocesnih rešenja za proizvodnju biopesticida na bazi biomase ili antimikrobnih jedinjenja koja proizvode antagonisti roda Bacillus, a koji pokazuju visoku efikasnost u suzbijanju bakteriozne pegavosti paprike.
Reference
*** (2010) Microsoft® Excel 2010 software, v. 2010. USA: MS Office, Microsoft Corporaton, https://office.com
*** (2018) Statistica (Data Analysis Software System). USA: TIBCO Software Inc, v.13.5., (http://tibco.com)
Bashan, Y., Diab, S., Okon, Y. (1982) Survival of Xanthomonas campestris pv.vesictoria in pepper seeds and roots in symptomless and dry leaves in non-host plants and in the soil. Plant and Soil, 68(2), 161-170
Buonaurio, R., Scarponi, L., Ferrara, M., Sidoti, P., Bertona, A. (2002) Induction of systemic acquired resistance in pepper plants by acibenzolar-S-methyl against bacterial spot disease. European Journal of Plant Pathology, 108, 41-49
de Vos, P., Garrity, G.M., Jones, D., Krieg, N.R., Ludwig, W., Rainey, F.A., Schleifer, K.H., Whitman, W.B., ur. (2009) Bergey's manual of systematic bacteriology. New York, NY: Springer Science, Volume 3; 2nd Edition
Earl, A.M., Losick, R., Kolter, R. (2008) Ecology and genomics of Bacillus subtilis. Trends in Microbiology, 16(6), 269-275
EPPO (2013) PM 7/110 (1) Xanthomonas spp. (Xanthomonas euvesicatoria, Xanthomonas gardneri, Xanthomonas perforans, Xanthomonas vesicatoria) causing bacterial spot of tomato and sweet pepper. OEPP/EPPO Bulletin, 43, 7-20
Fira, Đ., Dimkić, I., Berić, T., Lozo, J., Stanković, S. (2018) Biological control of plant pathogens by Bacillus species. Journal of Biotechnology, 285, 44-55
Gao, H., Li, P., Xu, X., Zeng, Q., Guan, W. (2018) Research on volatile organic compounds from Bacillus subtilis CF-3: Biocontrol effects on fruit fungal pathogens and dynamic changes during fermentation. Frontiers in Microbiology, 9, 456
Gašić, K., Kuzmanović, N., Ivanović, M., Prokić, A., Šević, M., Obradović, A. (2018) Complete genome of the Xanthomonas euvesicatoria specific bacteriophage KPh1, its survival and potential in control of pepper bacterial spot. Frontiers in Microbiology, 9, 2021
Köhl, J., Kolnaar, R., Ravensberg, W.J. (2019) Mode of action of microbial biological control agents against plant diseases: Relevance beyond efficacy. Frontiers in Plant Science, 10, 845
Mansfield, J., Genin, S., Magori, S., Citovsky, V., Sriariyanum, M., Ronald, P., Dow, M., Verdier, V., Beer, S.V., Machado, M.A., Toth, I., Salmond, G., Foster, G.D. (2012) Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular Plant Pathology, 13(6), 614-629
Moretti, C., Amatulli, M.T., Buonaurio, R. (2009) PCR-based assay for the detection of Xanthomonas euvesicatoria causing pepper and tomato bacterial spot. Letters in Applied Microbiology, 49(4), 466-471
Mota, M.S., Gomes, C.B., Souza, J.I.T., Moura, A.B. (2017) Bacterial selection for biological control of plant disease: criterion determination and validation. Brazilian Journal of Microbiology, 48(1), 62-70
Ongena, M., Jacques, P. (2008) Bacillus lipopeptides: Versatile weapons for plant disease biocontrol. Trends in Microbiology, 16(3), 115-125
Pajčin, I., Rončević, Z., Dodić, J., Dodić, S., Jokić, A., Grahovac, J. (2018) Production of biocontrol agents using Bacillus sp. in a laboratory-scale bioreactor. Journal on Processing and Energy in Agriculture, vol. 22, br. 3, str. 138-142
Potnis, N., Timilsina, S., Strayer, A., Shantharaj, D., Barak, J.D., Paret, M.L., Vallad, G.E., Jones, J.B. (2015) Bacterial spot of tomato and pepper: Diverse Xanthomonas species with a wide variety of virulence factors posing a worldwide challenge. Molecular Plant Pathology, 16(9), 907-920
Savary, S., Ficke, A., Aubertot, J., Hollier, C. (2012) Crop losses due to diseases and their implications for global food production losses and food security. Food Security, 4(4), 519-537
Shafi, J., Tian, H., Ji, M. (2017) Bacillus species as versatile weapons for plant pathogens: A review. Biotechnology & Biotechnological Equipment, 31(3), 446-459
Stamenković-Stojanović, S., Karabegović, I., Beškoski, V., Nikolić, N., Lazić, M. (2019) Bacillus based microbial formulations: Optimization of the production process. Hemijska Industrija (Chemical Industry), 73(3), 169-182
Stein, T. (2005) Bacillus subtilis antibiotics: Structures, syntheses and specific functions. Molecular Microbiology, 56(4), 845-857
Sundin, G.W., Castiblanco, L.F., Yuan, X., Zeng, Q., Yang, C. (2016) Bacterial disease management: Challenges, experience, innovation and future prospects. Molecular Plant Pathology, 17(9), 1506-1518
Sundin, G.W., Wang, N. (2018) Antibiotic resistance in plant-pathogenic bacteria. Annual Review of Phytopathology, 56(1), 161-180
Šević, M., Gašić, K., Đorđević, M., Ignjatov, M., Mijatović, M., Zečević, B., Obradović, A. (2016) Efficacy of biocontrol agents and bactericides in control of pepper bacterial spot. Acta Horticulturae, 1142(1142), 147-150
Šević, M., Gašić, K., Ignjatov, M., Mijatović, M., Prokić, A., Obradović, A. (2019) Integration of biological and conventional treatments in control of pepper bacterial spot. Crop Protection, 119, 46-51
 

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/jpea24-27076
primljen: 14.06.2020.
prihvaćen: 20.10.2020.
objavljen u SCIndeksu: 29.12.2020.