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2012, vol. 62, br. 5-6, str. 495-509
Promjene koncentracije imunoglobulina u nerastova primjenom levamisola i egzogenog adrenokortikotropnog hormona
aCroatian Veterinary Institute, Zagreb, Croatia
bUniversity of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, Croatia
Ključne reči: ACTH; albumins; boars; globulins; immunoglobulins; levamisole; total proteins
Cilj ovog istraživanja bio je utvrditi utjecaj levamisola (LEV) na koncentraciju imunoglobulina u serumu nerastova izloženih tri dana stresu administracijom ACTH. Nerastovi su podijeljeni u 4 skupine (n=7). Prva eksperimentalna grupa primala je LEV kroz 3 dana (2,5 mg/kg tj. mase), druga grupa tretirana je s ACTH (10 μg/kg tj. mase) tijekom 3 dana, a treća grupa kombinacijom LEV (2,5 mg/kg tj. mase) kroz 3 dana i zatim s ACTH (10 μg/kg tj. mase) 3 sljedeća dana (LEV+ ACTH). Kontrolna grupa primala je fiziološku otopinu tijekom 6 dana. Tijekom tretmana i 16 dana nakon tretmana određivane su koncentracije kortizola, ukupnih proteina, albumina i globulina, te imunoglobulina (IgG, IgA i IgM). Koncentracija kortizola je bila povećana u obje grupe tretirane s ACTH tijekom sva tri dana tretmana (p<0,05) te dan nakon posljednje primjene ACTH (p<0,05). Primjena ACTH utjecala je na povećanje ukupnih proteina tijekom primjene ACTH te tijekom 16 dana nakon tretmana (p<0,05). Međutim, u grupi nerastova tretiranih s LEV+ACTH ukupni proteini povišeni su samo na dane 1 i 2 primjene ACTH (p<0,05) te nakon završetka tretmana na dane 11 i 22 (p<0,05). Primjena LEV stimulirala je povećanje koncentracije proteina u odnosu na kontrolne nerastove nakon tretmana na dane 5, 14, 18 i 22 (p<0,05, svi). Primjena LEV i ACTH nije utjecala na koncentraciju albumina. Koncentracija globulina je povećana tijekom tretmana te 16 dana nakon primjene u nerastona tretiranih s ACTH i LEV+ACTH (p<0,05, svi). Koncentracija globulina se nije razlikovala između LEV i kontrolne grupe nerastova. Primjena ACTH utjecala je na povećanje razina IgG tijekom administracije ACTH te tijekom 16 dana nakon tretmana (p<0,05, svi). Međutim, u grupi životinja LEV+ACTH koncentracija IgG je bila povišena tijekom 1. i 3. dana tretmana s ACTH (p<0,05), te na dane 1 i 5 u periodu nakon tretmana (p<0,05). LEV nije imao utjecaj na koncentracije IgG u odnosu na kontrolu grupu nerastova. Povećane koncentracije IgA određene su na dane 2 i 11 (p<0,05) nakon primjene LEV u odnosu na životinje LEV+ACTH grupe. Rezultati ukazuju da primjena LEV može imati svrhu prevencije negativnog utjecaja stresa te inducuranja boljeg odgovora nespecifične imunosti u svinja.
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O članku

jezik rada: engleski
vrsta rada: neklasifikovan
DOI: 10.2298/AVB1206495B
objavljen u SCIndeksu: 22.03.2013.