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Journal of Agricultural Sciences (Belgrade)
2019, vol. 64, br. 4, str. 367-380
Morfološka karakterizacija i karakterizacija markerom SSR divljih i uzgajanih vigni (Vigna unguiculata L. Walp)
Ogunkanmi Adebayo L.a, Ogundipe Oluwatoyin T.b, Omoigui Luky O.c, Odeseye Adebola O.d, Fatokun Christian A.e
aUniversity of Lagos, Department of Cell Biology and Genetics, Nigeria
bUniversity of Lagos, Department of Botany, Nigeria
cInternational Institute of Tropical Agriculture, Ibadan, Nigeria
dNigerian Institute of Science Laboratory Technology, Department of Biological Science, Nigeria
eInternational Institute of Tropical Agriculture, Kano, Nigeria

e-adresa: adebayoogunkanmi@yahoo.com
Sažetak
Tristadevedeset genotipova koji su obuhvatali 260 gajenih i 130 genotipova divlje vigne fenotipski su ocenjivani korišćenjem 27 deskriptora vigne. Morfološka evaluacija nekih kvalitativnih osobina otkrila je od 11,92% do 29,23% prisustvo pigmentacije na stabljici, 1,53% do 20,76% prisustvo pruga na mahuni i 0% do 20% prisustvo dlakavosti na biljci gajene odnosno divlje vigne. U pogledu molekularne analize, šesnaest SSR prajmera su koršćeni za genotipizaciju 48 genotipova koji pripadaju kako divljoj tako i gajenoj vigni. Podaci su dali dendogram sa tri klastera, od kojih su se dva sastojala od divlje vigne dok je treći obuhvatao sve gajene vigne, uključujući i genotipove sa dugačkom mahunom (Vigna unguiculata subsp. sesquipedalis) i Vigna unguiculata podvrste cylindrica. Dva divlja genotipa podvrste dekindtiana, i po jedan iz podvrsta kgalagadensis i protracta grupisali su se sa genotipovima gajenih vigni ukazujući na njihove veze, ali ne i sa drugim divljim vignama. Broj polimorfnih traka SSR kod gajenih i divljih vigni bio je 38 odnosno 54, dok su vrednosti PIC bile 4,47 odnosno 6,14, što pokazuje veću genetsku raznovrsnost kod divljih nego kod gajenih vigni. Podvrsta dekindtiana imala je najveći broj (80%) prikazanih traka primenom SSR markera sa gajenom vignom, a zatim je sledila podvrsta protracta sa 54% prikazanih traka. Pet vrsta divlje vigne ima dlake, te bi se tako mogle koristiti u oplemenivanju za otpornost prema insektima.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/JAS1904367O
objavljen u SCIndeksu: 05.02.2020.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

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