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2017, vol. 33, br. 3, str. 321-332
Genetska varijacija japanske prepelice (Coturnix coturnix Japonica) bazirana na biohemijskom polimorfizmu
University of Ibadan, Animal Breeding and Genetics Unit, Department of Animal Science, Ibadan, Nigeria

e-adresacosamede@yahoo.com
Ključne reči: dendogram; japanska prepelica; polimorfizam; karakterizacija; elektroforeza
Sažetak
Cilj istraživanja je karakterizacija japanske prepelica koristeći biohemijske markere. Polimorfizam krvnih proteina od sto šezdeset šest (166) japanskih šarenih smeđih i belih prepelica, 83 svakog pola, analizirani su pomoću elektroforeze celuloznog acetat papira. Ispitivano je šest lokusa koji uključuju hemoglobin (Hb), transferin (Tf), albumin (Alb), karbonatnu anhidrazu (CA), alkalne fosfataze (Alp) i esteraze-1 (Es-1). Svi testirani lokusi su polimorfni sa svakim lokusom koji ima dva ko-dominantna alela koja kontrolišu tri genotipa. Alel B je dominantan za Hb, Tf i Es-1 lokus sa frekvencijama 0,90, 0,55 i 0,77, dok alel A preovlađuje na Alb i Alpu lokusima sa frekvencijama 0,83 i 0,58 respektivno. Alel A imao je generalno niže frekvencije od B u CA lokusima sa vrednostima od 0,43 - Braon, 0,38 - Bela i 0,40 - ukupno. Prosečna opažena heterozigotnost (Hₒ) bila je 0,48 sa smeđim i belim prepelicama sa vrednostima Ho 0,47 i 0,49 respektivno, a očekivana heterozigotnost je bila viša kod belih prepelica (0,39) nego u šareno smeđim (0,31). Genetička distanca (0,0534) između bele i smeđe prepelice u ovoj studiji pokazala je malo genetske diferencijacije između. Dendogram koji je generisan iz vrednosti genetičke distance pokazuje da su dva tipa imala zajedničko poreklo.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/BAH1703321A
objavljen u SCIndeksu: 15.12.2017.
Creative Commons License 4.0

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