Metrika

  • citati u SCIndeksu: 0
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[]
  • posete u poslednjih 30 dana:4
  • preuzimanja u poslednjih 30 dana:1

Sadržaj

članak: 3 od 18  
Back povratak na rezultate
2016, vol. 73, br. 2, str. 146-151
Određivanje flumazenila u serumu tečnom hromatografijom sa masenom spektrometrijom - primena u kinetičkoj studiji kod akutnog predoziranja diazepamom
aVojnomedicinska akademija, Nacionalni centar za kontrolu trovanja, Beograd + Univerzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd
bUniverzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd

e-adresaivezicnela@yahoo.com
Sažetak
Uvod/Cilj. Flumazenil je antagonist benzodiazepinskih receptora čiji su efekti ispitivani kod različitih indikacija kao što su reverzija sedacije posle hirurških intervencija ili dijagnostičkih procedura, terapija kome u akutnim trovanjima ili simptomatska terapija hepatične encefalopatije. Pojedini lekovi, kao što je teofilin, mogu dovesti do produženja poluvremena eliminacije flumazenila. Imajući u vidu dugo poluvreme eliminacije diazepama i njegovih metabolita, istovremena upotreba teofilina sa flumazenilom bi smanjila potrebu za ponovljenim davanjem flumazenila kod bolesnika sa akutnim trovanjem diazepamom. Stoga, cilj ovog rada bio je uvođenje pouzdane i precizne metode za određivanje koncentracije flumazenila u krvi nakon terapijske primene kod bolesnika sa akutnim trovanjem, a zatim, primenom ove metode utvrđivanje da li dolazi do izmena u kinetici flumazenila u prisustvu istovremeno primenjivanog aminofilina (kombinacija teofilina i etilendiamina u odnosu 2 : 1). Metode. Uzorci krvi bolesnika sa akutnim trovanjem diazepamom koji su dobili samo flumazenil u dozi od 0,5 mg ili istovremeno sa 3 mg/kg aminofilina, uzeti su 1, 3, 10, 30, 60, 120 i 240 min nakon njegove intravenske primene. Uzorci su pripremani čvrsto-faznom ekstrakcijom(SPE) na Oasis HLB kertridžima sa etilacetatom kao ekstrakcionim agensom. Flumazenil je određen tečnom hromatografijom sa masenom spektrometrijom (LC-MS) u single ion monitoring (SIM) modu na m/z 304. Razdvajanje flumazenila od komponenti matriksa izvršeno je na Lichrospher RP-8 koloni uz korišćenje smeše kiselog acetonitrila i 20 mM amonijum acetata u vodi (55 : 45) kao mobilne faze. Rezultati. Primenjena analitička metoda pokazala je odličan analitički prinos (94.65%). Dobijeni ekstrakti bili su čistiji nego ekstrakti dobijeni pomoću istog ekstraktanta nakon tečno-tečne ekstrakcije. Limit detekcije i limit kvantifikacije (LoQ) opisane LC-MS metode bili su 0,5 ng / mL i 1 ng/mL. Kod bolesnika lečenih istovremenom primenom flumazenila i aminofilina, konstanta eliminacije za flumazenil bila je značajno veća, a poluvreme eliminacije značajno duže u odnosu na ove parametre praćene u grupi bolesnika koja je pnmila samo flumazenil (p < 0,05). Zaključak. Primenjena LC-MS metoda za određivanje flumazenila u serumu bolesnika sa akutnim trovanjem diazepamom je brza, osetljiva, precizna i specifična. Istovremena primena teofilina značajno produžava eliminaciju flumazenila prilikom lečenja akutnih trovanja diazepamom.
Reference
*** (1987) Anexate (Flumazenil) product monograph. Basle, Switzerland: F. Hoffmann-La Roche & Co. Limited Company, [published 2008 December 30]. Available from: http://rochecanada.com/fmfiles/re7234008/Research/ClinicalTrialsForms/Products/Consumerinformation/Monographsand-PublicAdvisories/Anexate/anexateJune3pmE
Als-Nielsen, B., Kjaergard, L., Gluud, C. (2001) Benzodiazepine receptor antagonists for acute and chronic hepatic encephalopathy. Cochrane Database Syst Rev, (4): CD002798
Bonfiglio, M.F., Fisher-Katz, L.E., Saltis, L.M., Traeger, S.M., Martin, B.R., Nackes, N.A., i dr. (1996) A pilot pharmacokinetic-pharmacodynamic study of benzodiazepine antagonism by flumazenil and aminophylline. Pharmacotherapy, 16(6); 1166-72
Bun, H., Duplan, V., Crevat-Pisano, P., Llurens, M., Durand, A. (1989) Rapid determination of the benzodiazepine antagonist flumazenil, Ro 15-1788, by high performance liquid chromatography. Biomedical Chromatography, 3(6): 269-271
Chan, K., Jones, R.D.M. (1993) Simultaneous determination of flumazenil, midazolam and metabolites in human biological fluids by liquid chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 619(1): 154-160
Dahaba, A.A., Bornemann, H., Rehak, P.H., Wang, G., Wu, X.M., Metzler, H. (2009) Effect of Flumazenil on Bispectral Index Monitoring in Unpremedicated Patients. Anesthesiology, 110(5): 1036-1040
Djordjevic, S., Kovacevic, I., Miljkovic, B., Vuksanovic, J., Pokrajac, M. (2005) Liquid chromatographic-mass spectrometric method for the determination of fluoxetine and norfluoxetine in human plasma: application to clinical study. Il Farmaco, 60(4): 345-349
Fisher, L.E., Perch, S., Bonfiglio, M.F., Geers, S.M. (1995) Simultaneous determination of midazolam and flumazenil concentrations in human plasma by gas chromatography. Journal of Chromatography B: Biomedical Sciences and Applications, 665(1): 217-221
Hoffman, E.J., Warren, E.W. (1993) Flumazenil: a benzodiazepine antagonist. Clinical pharmacy, 12(9): 641-56; quiz 699-701
Kanazawa, H., Nagata, Y., Matsushima, Y., Takai, N., Uchiyama, H., Nishimura, R., Takeuchi, A. (1993) Liquid chromatography-mass spectrometry for the determination of medetomidine and other anaesthetics in plasma. Journal of Chromatography A, 631(1-2): 215-220
Kim, D.W., Joo, J.D., In, J.H., Jeon, Y.S., Jung, H.S., Jeon, K.B., Park, J.S., Choi, J.W. (2013) Comparison of the recovery and respiratory effects of aminophylline and doxapram following total intravenous anesthesia with propofol and remifentanil. Journal of Clinical Anesthesia, 25(3): 173-176
Kim, Y.J., Lee, H., Kim, C.H., Lee, G.Y., Baik, H.J., Han, J.I. (2012) Effect of flumazenil on recovery from anesthesia and the bispectral index after sevoflurane/fentanyl general anesthesia in unpremedicated patients. Korean Journal of Anesthesiology, 62(1): 19
Kleingeist, B., Bocker, R., Geisslinger, G., Brugge, R. (1998) Isolation and pharmacological characterization of microsomal human liver flumazenil carboxylesterase. J Pharm Pharmaceut Sci, 1(1); 38-46
Klotz, U., Duka, T., Dorow, R., Doenicke, A. (1985) Flunitrazepam and lormetazepam do not affect the pharmacokinetics of the benzodiazepine antagonist Ro 15-1788. British Journal of Clinical Pharmacology, 19(1): 95-98
Klotz, U., Kanto, J. (1988) Pharmacokinetics and Clinical Use of Flumazenil (Ro 15-1788). Clinical Pharmacokinetics, 14(1): 1-12
Kratzsch, C., Tenberken, O., Peters, F.T., Weber, A.A., Kraemer, T., Maurer, H.H. (2004) Screening, library-assisted identification and validated quantification of 23 benzodiazepines, flumazenil, zaleplone, zolpidem and zopiclone in plasma by liquid chromatography/mass spectrometry with atmospheric pressure chemical ionization. Journal of Mass Spectrometry, 39(8): 856-872
Krisanda, T.J. (1993) Flumazenil: an antidote for benzodiazepine toxicity. Am Fam Physician, 47(4); 891-5; 6
Laven, M., Markides, K., Langstrom, B. (2004) Analysis of microsomal metabolic stability using high-flow-rate extraction coupled to capillary liquid chromatography-mass spectrometry. Journal of Chromatography B, 806(2): 119-126
Laven, M. (2004) Determination of flumazenil in human plasma by liquid chromatography?electrospray ionisation tandem mass spectrometry. Journal of Chromatography B, 808(2): 221-227
Lopez, A., Rebollo, J. (1990) Benzodiazepine withdrawal syndrome after a benzodiazepine antagonist. Critical Care Medicine, 18(12): 1480
Marchant, B., Wray, R., Leach, A., Nama, M. (1989) Flumazenil causing convulsions and ventricular tachycardia. BMJ, 299(6703): 860-860
Mégarbane, B., Buisine, A., Jacobs, F., Résière, D., Chevillard, L., Vicaut, E., Baud, F.J. (2010) Prospective comparative assessment of buprenorphine overdose with heroin and methadone: Clinical characteristics and response to antidotal treatment. Journal of Substance Abuse Treatment, 38(4): 403-407
Najjar, T.A., Al-Hassan, M.I., Khan, R.M.A. (1993) Theophylline inhibits the elimination of flumazenil in rabbits. International Journal of Pharmaceutics, 98(1-3): 51-55
Scheepers, L.D., Montgomery, C.J., Kinahan, A.M., Dunn, G.S., Bourne, R.A., McCormack, J.P. (2000) Plasma concentrations of flumazenil following intranasal administration in children. Canadian Journal of Anesthesia/Journal canadien d'anesthésie, 47(2): 120-124
Song, D., Khaykis, V., Kohlhof, K. (1995) Determination of flumazenil in plasma by gas chromatography-negative ion chemical ionization mass spectrometry. Journal of Chromatography B: Biomedical Sciences and Applications, 663(2): 263-273
Thomson, J.S., Donald, C., Lewin, K. (2006) Use of Flumazenil in Benzodiazepine overdose. Emerg Med J, 23(2); 162
Turan, A., Memis, D., Karamanlyodlu, B., PamukCu, Z., Sut, N. (2004) Effect of aminophylline on bispectral index. Acta Anaesthesiologica Scandinavica, 48(4): 408-411
Vletter, A.A., Burm, A.G.L., Breimer, L.T.M., Spierdijk, Joh. (1990) High-performance liquid chromatographic assay to determine midazolam and flumazenil simultaneously in human plasma. Journal of Chromatography B: Biomedical Sciences and Applications, 530: 177-185
Votey, S.R., Bosse, G.M., Bayer, M.J., Hoffman, J.R. (1991) Flumazenil: A new benzodiazepine antagonist. Annals of Emergency Medicine, 20(2): 181-188
Weinbroum, A., Rudick, V., Sorkine, P., Nevo, Y., Halpern, P., Geller, E., Niv, D. (1996) Use of flumazenil in the treatment of drug overdose. Critical Care Medicine, 24(2): 199-206
Whitwam, J.G., Amrein, R. (1995) Pharmacology of flumazenil. Acta Anaesthesiologica Scandinavica, 39: 3-14
Wille, R.T., Chaffee, B.W., Ryan, M.L., Elta, G.H., Walter, V., Barnett, J.L. (2000) Pharmacoeconomic evaluation of flumazenil for routine outpatient EGD. Gastrointestinal Endoscopy, 51(3): 282-287
Woolf, A.D., Erdman, A.R., Nelson, L.S., Caravati, M.E., Cobaugh, D.J., Booze, L.L., Wax, P.M., Manoguerra, A.S., Scharman, E.J., Olson, K.R. (2007) Tricyclic antidepressant poisoning: An evidence-based consensus guideline for out-of-hospital management*. Clinical Toxicology, 45(3): 203-233
Zedkova, L., Rauw, G.A., Baker, G.B., Coupland, N.J. (2001) A rapid high-pressure liquid chromatographic procedure for determination of flumazenil in plasma. Journal of Pharmacological and Toxicological Methods, 46(1): 57-60
 

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/VSP141222019D
objavljen u SCIndeksu: 03.02.2016.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

Povezani članci

Nema povezanih članaka