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2020, vol. 9, br. 1, str. 27-36
Hemijski sastav, antioksidativna i antimikrobna aktivnost etarskog ulja i ekstrakta najkvirca (Pimenta dioica (L.) Merr.)
aUniverzitet u Nišu, Tehnološki fakultet, Leskovac
bUniverzitet u Nišu, Prirodno-matematički fakultet

e-adresastanojevic@tf.ni.ac.rs
Projekat:
Ministarstvo prosvete, nauke i tehnološkog razvoja Republike Srbije (institucija: Univerzitet u Nišu, Tehnološki fakultet, Leskovac) (MPNTR - 451-03-68/2020-14/200133)

Sažetak
Plod najkvirca (Pimenta dioica (L.) Merr.) korišćen je za izolaciju etarskog ulja i etanolnog ekstrakta u ovom istraživanju. Etarsko ulje je dobijeno Clevenger hidrodestilacijom iz ploda najkvirca pri hidromodulu 1:10 m/v u toku 180 minuta, dok je etanolni ekstrakt dobijen refluks ekstrakcijom na temperaturi ključanja pri solvomodulu 1:10 m/v u toku 120 minuta. Kvalitativni i kvantitativni sastav dobijenog etarskog ulja određen je GC/MS i GC/FID metodama, respektivno. Sadržaj ukupnih fenola i flavonoida određen je spektrofotometrijski, Folin-Ciocalteu i AlCl3 , metodoma, respektivno. Antioksidativna aktivnost etarskog ulja i ekstrakta ploda najkvirca određena je spektrofotometrijski primenom DPPH testa, dok je antimikrobna aktivnost određena mikrodilucionom metodom na sledeće mikroorganizme: dve vrste bakterija (Bacillus cereus, Salmonella spp.) i jedan kvasac (Candida albicans), pri čemu su testirani parovi referentnih sojeva i njihovih izolata. Na osnovu dobijenih rezultata utvrđeno je prisustvo 20 komponenti u etarskom ulju, uglavnom oksigenovanih monoterpena sa najvišim sadržajem eugenola (48,5%) i metil eugenola (35,0%). Ekstrakt i etarsko ulje su pokazali najbolju antioksidativnu aktivnost posle 20 i 60 minuta inkubacije sa EC50 vrednostima 0,011 mg/cm³ i 0,023 mg/cm³, respektivno. Etarsko ulje i ekstrakt pokazali su inhibitorno dejstvo na sve ispitivane mikroorganizme. Etanolni ekstrakt najkvirca pokazao je bolji inhibitorni efekat na B. cereus i S. enterica (izolat i ATCC sojeve) od etarskog ulja. Etarsko ulje najkvirca pokazalo je bolji inhibitorni efekat na sojeve C. albicans. Dobijeni rezultati ukazuju na to da su i etarsko ulje i ekstrakt izolovani iz aromatičnog ploda najkvirca dobar izvor prirodnih antioksidanasa i antimikrobnih sredstava sa potencijalnom primenom u prehrambenoj i farmaceutskoj industriji kao sigurnija alternativa sintetičkim aditivima.
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jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/savteh2001027M
objavljen u SCIndeksu: 13.07.2020.

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