- citati u SCIndeksu: [2]
- citati u CrossRef-u:0
- citati u Google Scholaru:[
 ]
- posete u poslednjih 30 dana:7
- preuzimanja u poslednjih 30 dana:5
|
|
|
2019, vol. 34, br. 1, str. 39-46
|
|
Antifungalno delovanje etarskih ulja na Verticillium dahliae Klebahn, prouzrokovača verticilioznog uvenuća paprike
Antifungal activity of plant essential oils against Verticillium dahliae Klebahn, the causal agent of Verticillium wilt of pepper
aInstitut za pesticide i zaštitu životne sredine, Beograd, Srbija bInstitut za proučavanje lekovitog bilja 'Dr Josif Pančić', Beograd, Srbija
e-adresa: ivana.potocnik@pesting.org.rs
Sažetak
Biofungicidi bazirani na biljnim uljima imaju prednost u odnosu na hemijske fungicide, posebno kada se uzme u obzir njihovo štetno dejstvo na životnu sredinu. Primenom makrodilucione fumigantne metode ispitana je inhibitorna i fungicidna aktivnost 22 etarska ulja iz Nemačke i Albanije na Verticillium dahlia Klebahn, prouzrokovača verticilioznog uvenuća paprike. Efikasnost etarskih ulja testirana je na različitim koncetracijama (0,02; 0,04; 0,08; 0,16 i 0,32 μl ml-1 vazdušne faze) i na osnovu njih su određene minimalne inhibitorne i fungicidne koncetracije. Najjače dejstvo ispoljila su dva uzorka etarskog ulja nane (iz Nemačke i Albanije), oba sa minimalnim inhibitornim i fungicidnim koncetracijama od 0,02 μl ml-1 vazdušne faze, zatim etarska ulja eukaliptusa, crnog bora i crvene kleke. Sva nabrojana ulja bila su letalna za patogenu gljivu. Devet od 22 ispitivana etarska ulja ispoljila su inhibitorni efekat: dva uzorka nane, crvena kleka, eukaliptus, crni bor, lavanda, žalfija i ruzmarin, dok je pet etarskih ulja ispoljilo i fungicidni efekat (dva uzorka nane, eukaliptus, crni bor i crvena kleka). Rezultati ukazuju da su ulja nane, eukaliptusa, crnog bora i crvene kleke pogodna za dalja ispitivanja in vivo testovima protiv prouzrokovača verticilioznog uvenuća paprike (Verticillium dahlia).
Abstract
Biofungicides based on plant oils have some advantages compared to chemical fungicides, especially considering their harmful effect on the environment. Twenty-two essential oils from Germany and Albania were assayed for inhibitory and fungicidal activity against Verticillium dahliae Klebahn, the causal agent of Verticillium wilt of pepper, using the macrodilution fumigant method. The concentrations of oils obtained in the vapour phase were: 0.02, 0.04, 0.08, 0.16 and 0.32 μl ml-1 with determined minimum inhibitory and fungicidal concentrations. The strongest activity was shown by two samples of mint oil (Mentha piperita L.) at 0.02 μl ml-1 of air, both from Germany and Albania, followed by plant essential oils of eucalyptus (Eucalyptus globulus Labilardie), black pine (Pinus nigra L.) and cade (Juniperus oxycedrus L.), and all of them were lethal to the pathogen. Nine oils: two samples of mint, cade, eucalyptus, black pine, lavender (Lavandula angustifolia Mill.), sage (Salvia officinalis L.) and rosemary (Rosmarinus officinalis L.) inhibited the growth of Verticillium dahliae, while five oils (two samples of mint, eucalyptus, black pine and cade) showed fungicidal effects on the pathogen. These results showed that mint, eucalyptus, black pine and cade essential oils have a potential for further in vivo experiments against Verticillium dahliae.
|
|
|
|
Reference
|
|
  
|
Abada, K.A. (2014) Management Fusarium Wilt of Sweet Pepper by Bacillus Strains. American Journal of Life Sciences, 2(6): 19-25
|
|
|
Abada, K.A., Eman, H.O. (2017) Effect the combination among compost, bioagents and soil solarization on management of strawberry Verticillium wilt. International Journal of Scientific and Engineering Research, 8(2): 88-99
|
|
|
Abada, K.A.M., Attia, A.M.F., El-Fiki, I.A.M. (2018) Bacterial Bioagents and Compost as Two Tools for Management of Eggplant Fusarium Wilt. Frontiers in Environmental Microbiology, 4(1): 1-10
|
|
|
Abou-Jawdah, Y., Sobh, H., Salameh, A. (2002) Antimycotic Activities of Selected Plant Flora, Growing Wild in Lebanon, against Phytopathogenic Fungi. Journal of Agricultural and Food Chemistry, 50(11): 3208-3213
|
|
|
Adams, R.P. (2007) Identification of essential oil components by gas chromatography/mass spectrometry. Carol Stream, IL, USA: Allured Publishing Corporation, 4th ed
|
|
3
|
Arslan, M., Dervis, S. (2010) Antifungal activity of essential oils against three vegetative-compatibility groups of Verticillium dahliae. World Journal of Microbiology and Biotechnology, 26(10): 1813-1821
|
|
|
Bayan, Y., Yilar, M., Onaran, A. (2016) Antifungal activity and chemical composition of the essential oil of Heracleum platytaenium Biss's. Egytian Journal of Biological Pest Control, 26(2): 237-240
|
|
|
Byron, E.M., Hall, A.M. (2002) Inhibition of common cereal pathogenic fungi by clove oil and eucalyptus oil. u: Margini T. [ur.] Proceedings of Brighton Crop Protection Conference - Pest and Diseases, Brighton, UK: University of Brighton Press, 2 (pp. 765-768)
|
|
28
|
Dhingra, O.D., Sinclair, J.B. (1995) Basic plant pathology methods. Boca Raton, FL: CRC Press
|
|
4 
|
Đurović-Pejčev, R., Potočnik, I., Milijašević-Marčić, S., Todorović, B., Rekanović, E., Stepanović, M. (2014) Antifungal activity of six plant essential oils from Serbia against Trichoderma aggressivum f. europaeum. Pesticidi i fitomedicina, vol. 29, br. 4, str. 291-297
|
|
|
Garcia-Rellán, D., Verdeguer, M., Salamone, A., Blázquez, M.A., Boira, H. (2016) Chemical Composition, Herbicidal and Antifungal Activity of Satureja cuneifolia Essential Oils from Spain. Natural Product Communications, 11(6), 841-844
|
|
|
Goldberg, N.P. (2003) Verticillium wilt. u: Pernezny K.L., Roberts P.D., Murphy J.F., Goldberg N.P. [ur.] Compendium of pepper diseases, St. Paul, MN: American Phytopathological Society, pp 21-22
|
|
|
Hammami, I., Smaoui, I., Ben, S., Hsouna, A., Hamdi, N., Triki, A.M. (2015) Ruta montana L. leaf essential oil and extracts: characterization of bioactive compounds and suppression of crown gall disease. EXCLI Journal, 83-94; 14
|
|
|
Jayaraj, J., Wan, A., Rahman, M., Punja, Z.K. (2008) Seaweed extract reduces foliar fungal diseases on carrot. Crop Protection, 27(10): 1360-1366
|
|
|
Klosterman, S.J., Atallah, Z.K., Vallad, G.E., Subbarao, K.V. (2009) Diversity, Pathogenicity, and Management of Verticillium Species. Annual Review of Phytopathology, 47(1): 39-62
|
|
7
|
Kosanović, D., Potočnik, I., Duduk, B., Vukojević, J., Stajić, M., Rekanović, E., Milijašević-Marčić, S. (2013) Trichoderma species on Agaricus bisporus farms in Serbia and their biocontrol. Annals of Applied Biology, 163(2): 218-230
|
|
1
|
Luković, J., Stepanović, M., Todorović, B., Milijašević-Marčić, S., Duduk, N., Vico, I., Potočnik, I. (2018) Antifungal activity of cinnamon and clove essential oils against button mushroom pathogens Cladobotryum dendroides (Bull.) W. Gams & Hooz and Lecanicillium fungicola var. fungicola (Preuss) Hasebrauk. Pesticidi i fitomedicina, vol. 33, br. 1, str. 19-26
|
|
1
|
Milijašević-Marčić, S., Todorović, V., Stanojević, O., Berić, T., Stanković, S., Todorović, B., Potočnik, I. (2018) Antagonistic potential of Bacillus spp. isolates against bacterial pathogens of tomato and fungal pathogen of pepper. Pesticidi i fitomedicina, vol. 33, br. 1, str. 9-18
|
|
|
Pegg, G.F., Brady, B.L. (2002) Verticillium wilts. Wallingford: CABI Publishing
|
|
|
Pilar, S.M., Roselló, J. (2006) Influence of temperature and water activity on the antagonism of Trichoderma harzianum to Verticillium and Rhizoctonia. Crop Protection, 25(10): 1130-1134
|
|
|
Rekanovic, E., Milijasevic, S., Todorovic, B., Potocnik, I. (2007) Possibilities of biological and chemical control of Verticillium wilt in pepper. Phytoparasitica, 35(5): 436-441
|
|
2
|
Ślusarski, C., Pietr, S.J. (2009) Combined application of dazomet and Trichoderma asperellum as an efficient alternative to methyl bromide in controlling the soil-borne disease complex of bell pepper. Crop Protection, 28(8): 668-674
|
|
6
|
Soković, M., van Griensven, L.J.L.D. (2006) Antimicrobial activity of essential oils and their components against the three major pathogens of the cultivated button mushroom, Agaricus bisporus. European Journal of Plant Pathology, 116(3): 211-224
|
|
3
|
Talboys, P.W. (1984) Chemical control of Verticillium wilts. Phytopathologia Mediterranea, 23,163-175
|
|
|
Tanovic, B., Milijasevic, S., Obradovic, A. (2007) In vitro effect of plant essential oils on growth of some soil-borne pathogens. Acta Horticulturae, (729): 467-471
|
|
3
|
Tanović, B., Potočnik, I., Stanisavljević, B., Đorđević, M., Rekanović, E. (2006) Response of Verticillium fungicola var. fungicola, Mycogone perniciosa and Cladobotryum sp. Mushroom pathogens to some essential oils. Pesticidi i fitomedicina, vol. 21, br. 3, str. 231-237
|
|
3
|
Tian, L., Wang, K.R., Lu, J.Y. (1998) Effect of carbendazim and tricyclazole on microsclerotia and melanin formation of Verticillium dahliae. Acta Phytopathol. Sinica, 28, str. 263-268
|
|
4
|
Todorović, B., Potočnik, I., Rekanović, E., Stepanović, M., Kostić, M., Ristić, M., Milijašević-Marčić, S. (2016) Toxicity of twenty-two plant essential oils against pathogenic bacteria of vegetables and mushrooms. Journal of Environmental Science and Health, Part B, 51(12): 832-839
|
|
|
Varo, A., Mulero-Aparicio, A., Adem, M., Roca, L.F., Raya-Ortega, M.C., López-Escudero, F.J., Trapero, A. (2017) Screening water extracts and essential oils from Mediterranean plants against Verticillium dahliae in olive. Crop Protection, 92(): 168-175
|
|
|
|
|