• citations in SCIndeks: 0
  • citations in CrossRef:[1]
  • citations in Google Scholar:[]
  • visits in previous 30 days:10
  • full-text downloads in 30 days:8


article: 2 from 13  
Back back to result list
2020, vol. 57, iss. 3, pp. 80-86
Diversity of phytopathogenic fungi on soybean seed in Serbia
Institute of Field and Vegetable Crops, Novi Sad, Serbia
Ministry of Education, Science and Technological Development, Republic of Serbia (Institution: Institute of Field and Vegetable Crops, Novi Sad) (MESTD - 451-03-68/2020-14/200032)

Keywords: soybean; seed; phytopathogenic fungi; incidence of infection
Soybean is one of the most important industrial crops in the world. The rich nutritional content of the seed is the reason for the increased cultivation of this crop all over the world. However, a large number of phytopathogenic fungi that exist in soybean seeds can reduce the nutritional content, germination, and seed vigor. Soybean seed samples were collected from experimental fields in seven locations in Vojvodina Province, Serbia in the period 2016-2018. From each sample, 100 seeds were randomly selected. Seeds were surface-disinfested in 4% sodium hypochlorite for 5 min, washed two times in sterile water, and dried on a filter paper. All 100 seeds were placed in 20 Petri dishes with standard potato dextrose agar medium (PDA), five seeds in each, and incubated at 22 ± 2°C for seven days, under 12 h of alternating light/dark conditions. The first assessment was performed on the fourth day after isolation. The health status analysis of soybean seed showed that Peronospora manshurica, Macrophominaphaseolina, Botrytis cinerea, Cercospora kikuchii as well as the species from genera Alternaria, Diaporthe, and Fusarium had colonized soybean seed in Serbia over three years. Species from the genus Alternaria and Peronospora manshurica were dominant in all three examined years. Furthermore, it has been noticed that weather conditions, location, and cultivar significantly influenced the intensity of the infection. The results of this study have shown which pathogens pose a threat to successful soybean seed production and help in finding preventive measures to control these pathogens during vegetation.
Backman, P.A., Weaver, D.B., Morgan-Jones, G. (1985) Soybean Stem Canker: An Emerging Disease Problem. Plant Disease, 69(8): 641-647
Baird, R.E., Abney, T.S., Mullinix, B.G. (2001) Fungi associated with pods and seeds during the R6 and R8 stages of four soybean cultivars in southwestern Indiana. Phytoprotection, 82(1): 1-11
Balducchi, A.J., Mcgee, D.C. (1987) Environmental Factors Influencing Infection of Soybean Seeds by Phomopsis and Diaporthe Species During Seed Maturation. Plant Disease, 71(3): 209-212
Hartman, G.L., Rupe, J.C., Sikora, E.J., Domier, L.L., Davis, J.A., Steffey, K.L. (2015) Compendium of soybean diseases and Pests. St. Paul, Minnesota: American Phytopathological Society Press, Fifth edition
Jasnić, S.M., Marjanović, Ž.S., Vidić, M.B., Bagi, F.F., Budakov, D.B., Pavlović, S.Đ., Stojšin, V.B. (2011) Patogene morfološke i molekularne osobine Alternaria tenuissima sa soje / Pathogenic, morphological and molecular characteristics of Alternaria tenuissima from soybean. Zbornik Matice srpske za prirodne nauke, br. 120, str. 183-196
Koenning, S.R., Wrather, A.J. (2010) Suppression of Soybean Yield Potential in the Continental United States by Plant Diseases from 2006 to 2009. Plant Health Progress, 11(1): 5-5
Lee, J.H., Hwang, S.R., Lee, Y.H., Kim, K., Cho, K.M., Lee, Y.B. (2015) Changes occurring in compositions and antioxidant properties of healthy soybean seeds [Glycine max (L.) Merr.] and soybean seeds diseased by Phomopsis longicolla and Cercospora kikuchii fungal pathogens. Food Chem, 185: 205-11
Lević, J., Stanković, S., Krnjaja, V., Bočarov-Stančić, A., Ivanović, D. (2012) Distribution frequency and incidence of seed-borne pathogens of some cereals and industrial crops in Serbia. Pesticidi i fitomedicina, vol. 27, br. 1, str. 33-40
Medić-Pap, S., Milošević, M., Jasnić, S. (2007) Soybean seedborne fungi in the Vojvodina province. Phytopathologia Polonica, 45: 55-65
Miladinović, J., Hrustić, M., Vidić, M. (2011) Soybean. Novi Sad: Institute of Field and Vegetable Crops
Oladimeji, A., Olusegun, B.S., Oluyemisi, F.B., Oluwatoyin, F.A., Aliyu, H.T., Kassoum, K.O. (2016) Seed-borne fungi of soybeans (Glycine max [L.] merr) in the Guinea Savannah agroecology of Nigeria. Journal of Agricultural Sciences, vol. 61, br. 1, str. 57-68
Petrović, K., Riccioni, L., Vidić, M., Đorđević, V., Balešević-Tubić, S., Đukić, V., Miladinov, Z. (2016) First Report of Diaporthe novem, D. foeniculina, and D. rudis Associated with Soybean Seed Decay in Serbia. Plant Disease, 100(11): 2324-2324
Petrović, K., Vidić, M. (2010) Rasprostranjenost parazita semena soje u Srbiji / Distribution of soybean seed parasites in Serbia. in: Savetovanje o zaštiti bilja (X), Zlatibor, zbornik rezimea / Counselling of Plant Protection (X), Book of Abstracts, str. 108-109
Petrović, K., Vidić, M., Riccioni, L., Đorđević, V., Rajković, D. (2015) First Report of Diaporthe eres Species Complex Causing Seed Decay of Soybean in Serbia. Plant Disease, 99(8): 1186-1186
Petrović, K., Riccioni, L., Đorđević, V., Balešević-Tubić, S., Miladinović, J., Ćeran, M., Rajković, D. (2018) Diaporthe pseudolongicolla: The new pathogen on soybean seed in Serbia. Ratarstvo i povrtarstvo, vol. 55, br. 2, str. 103-109
Pioli, R.N., Grijalba, P., Gosfarini, C.O., Martinez, C., Tozzini, A., Hopp, E., Morandi, E.N. (1999) Morphological, pathogenic and molecular characterization of different isolates of Diaporthe phaseolorum var. meridional obtained in different areas of Santa Fe province, Argentina. in: World Soybean Res. Conf. VI, Chicago. IL, USA, Proc, str. 627-628
Roy, K.W., Baird, R.E., Abney, T.S. (2000) A review of soybean (Glycine max) seed, pod, and flower mycofloras in North America, with methods and a key for identification of selected fungi. Mycopathologia, 150, str. 15-27
Rupe, J.C., Ferris, R.S. (1986) Effect of pod moisture on soybean seed infection by Phomopsis sp. Phytopathology, 76(3): 273-273
Sinclair, J.B., Backman, P.A. (1989) A compendium of soybean diseases. St. Paul, Minnesota: American Phytopathological Society Press, 3 rd Edition
Spilker, D.A., Schmitthenner, A.F., Ellete, C.W. (1981) Effect of humidity, temperature, fertility and cultivar on the reduction of soybean seed quality by Phomopsis sp. Phytopathology, 71(10): 1027-1027
Talenko, D. (2018) Indiana Soybeans: Fungal diseases that impact pod, Seed Quality. Purdue University, Retrived from
Tenuta, A., Bradley, C., Chilver, M., Giesler, L., Mathew, F., Mueller, D., Sisson, A., Smith, D., Wise, K. (2015) Scouting for Common Soybean Seed Diseases. Crop Protection Network, Retrieved from
Vidić, M. (1992) Epidemiološke karakteristike najznačajnijih parazita soje u Vojvodini / Epidemiological characteristics of the most important soybean parasites in Vojvodina. Zbornik radova Instituta za ratarstvo i povrtarstvo, Novi Sad, 20: 519-522
Vidić, M., Jasnić, S., Đorđević, V. (2006) Rasportranjenost Diaporthe/Phomopsis vrsta na semenu soje u Srbiji / Distribution of seed-borne Diaporthe/Phomopsis species in soybean in Serbia. Pesticidi i fitomedicina, vol. 21, br. 1, str. 39-48
Vidić, M., Miladinović, J., Đorđević, V. (2002) Uticaj odlaganja žetve na intenzitet pojave truleži semena / Effect of harvest delay on the intensity of soya bean seed decay. Zaštita bilja, vol. 53, br. 1, str. 39-50
Vlahović, B., Ilin, S., Puškarić, A. (2013) Status and Perspectives of Soybean Production Worldwide and in the Republic of Serbia. Economic Insights: Trends & Challenges, 65 (1): 38-46
Wrather, A.J., Koenning, R.S. (2006) Estimates of disease effects on soybean yields in the United States 2003-2005. Journal of Nematology, 38: 173-180
Wrather, A.J., Shannon, G., Balardin, R., Carregal, L., Escobar, R., Gupta, G.K., Ma, Z., Morel, W., Ploper, D., Tenuta, A. (2010) Effect of diseases on soybean yield in the top eight producing countries in 2006. Plant Health Progress, 11(1)
Xue, A.G., Morrison, M.J., Cober, E., Anderson, T.R., Rioux, S., Ablett, G.R., Rajcan, I., Hall, R., Zhang, J.X. (2007) Frequency of isolation of species of Diaporthe and Phomopsis from soybean plants in Ontario and benefits of seed treatments. Canadian Journal of Plant Pathology, 29(4): 354-364


article language: English
document type: Original Scientific Paper
DOI: 10.5937/ratpov57-27516
received: 15/07/2020
accepted: 19/08/2020
published in SCIndeks: 25/12/2020
peer review method: double-blind
Creative Commons License 4.0

Related records

No related records