Metrika

  • citati u SCIndeksu: 0
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[]
  • posete u poslednjih 30 dana:14
  • preuzimanja u poslednjih 30 dana:5

Sadržaj

članak: 6 od 49  
Back povratak na rezultate
Pesticidi i fitomedicina
2019, vol. 34, br. 1, str. 61-67
Ispitivanje genotoksičnih efekata fungicida i njegovih aktivnih supstanci na mononuklearne ćelije ljudske krvi
Cobanoglu Hayala, Coskun Baverb, Cayir Akina
aÇanakkale Onsekiz Mart University, Vocational Health College, Terzioglu Campus, Çanakkale, Turkey
bÇanakkale Onsekiz Mart University, Faculty of Agriculture, Department of Animal Science, Terzioglu Campus, Çanakkale, Turkey

e-adresa: acincay79@yahoo.com
Sažetak
spitivani su genotoksični efekti fungicida Signum® i njegove dve aktivne supstance (piraklostrobin i boskalid) primenom komet testa (pH>13). Leukociti izolovani iz krvi tretirani su različitim koncentracijama (0.1-25 µg/ml) fungicida tokom 2 h i 20 h. Test sa tripansko plavim obojenjem pokazao je veću ćelijsku vijabilnost (>84%) kod sve tri koncentracije i u oba intervala primene. Rezultati su pokazali da Signum® i piraklostrobin dovode do statistički značajnog oštećenja. Nasuprot tome, boskalid nije doveo do statistički značajnog oštećenja DNK nakon 2 h izlaganja, iako je uzrokovao DNK oštećenja pri višim koncentracijama tokom dužeg izlaganja (20 h). Može se zaključiti da piraklostrobin i Signum® mogu imati genotoksično delovanje. Ipak, u okviru ispitivanog opsega koncentracija, nijedan od ispitivanih fungicida nije pokazao citotoksično delovanje tokom dva intervala primene.
Reference

Benedetti, D., Nunes, E., Sarmento, M., Porto, C., Santos, C.E.I.D., Dias, J.F., da Silva, J. (2013) Genetic damage in soybean workers exposed to pesticides: Evaluation with the comet and buccal micronucleus cytome assays. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 752(1-2): 28-33

Bokán, K., Syberg, K., Jensen, K., Rank, J. (2013) Genotoxic Potential of Two Herbicides and their Active Ingredients Assessed with Comet Assay on a Fish Cell Line, Epithelioma Papillosum Cyprini (EPC). Journal of Toxicology and Environmental Health, Part A, 76(20): 1129-1137

Bolognesi, C., Bonatti, S., Degan, P., Gallerani, E., Peluso, M., Rabboni, R., Roggieri, P., Abbondandolo, A. (1997) Genotoxic Activity of Glyphosate and Its Technical Formulation Roundup. Journal of Agricultural and Food Chemistry, 45(5): 1957-1962

Bony, S., Gaillard, I., Devaux, A. (2010) Genotoxicity assessment of two vineyard pesticides in zebrafish. International Journal of Environmental Analytical Chemistry, 90(3-6), 421-428

Bony, S., Gillet, C., Bouchez, A., Margoum, C., Devaux, A. (2008) Genotoxic pressure of vineyard pesticides in fish: Field and mesocosm surveys. Aquatic Toxicology, 89(3): 197-203

Çayır, A., Coskun, M., Coskun, M. (2014) Micronuclei, nucleoplasmic bridges, and nuclear buds induced in human lymphocytes by the fungicide signum and its active ingredients (boscalid and pyraclostrobin). Environmental Toxicology, 29(7), 723-732. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/22730168

Collins, A.R. (2004) The Comet Assay for DNA Damage and Repair: Principles, Applications, and Limitations. Molecular Biotechnology, 26(3): 249-261

Costa, S., Pina, C., Coelho, P., Costa, C., Silva, S., Porto, B., Laffon, B., Teixeira, J.P. (2011) Occupational Exposure to Formaldehyde: Genotoxic Risk Evaluation by Comet Assay and Micronucleus Test Using Human Peripheral Lymphocytes. Journal of Toxicology and Environmental Health, Part A, 74(15-16): 1040-1051

2

Cox, C., Surgan, M. (2006) Unidentified Inert Ingredients in Pesticides: Implications for Human and Environmental Health. Environmental Health Perspectives, 114(12): 1803-1806, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764160

de Barros, A.L., de Souza, V.V., Navarro, S.D., Oesterreich, S.A., Oliveira, R.J., Kassuya, C.A.L., Arena, A.C. (2013) Genotoxic and Mutagenic Effects of Diflubenzuron, an Insect Growth Regulator, on Mice. Journal of Toxicology and Environmental Health, Part A, 76(17): 1003-1006

Dhawan, A., Anderson, D., eds. (2009) The Comet Assay in Toxicology. u: Dhawan, A., & Anderson, D. [ur.] The comet assay in toxicology, London: Royal Society of Chemistry, Vol. 5

Dhawan, A., Bajpayee, M.M., Pandey, A.K., Parmar, D. (2003) Protocol for the single cell gel electrophoresis/comet assay for rapid genotoxicity assessment. SIGMA, 1077, 1

EPA (1997) Pesticide registration (PR) notice 97-6: Use of term 'inert' in the label ingredients statement. Retrieved from https://www.epa.gov/pesticide-registration/ prn-97-6-use-term-inert-label-ingredients-statement

EPA (2003) Pesticide fact sheet : Name of chemical: Boscalid. Retrieved from: https://www3.epa.gov/pesticides/chem_ search/reg_actions/registration/fs_PC-128008_01-Jul-03.pdf

EPA (2006) Terms of environment: Glossary, abbreviations and acronyms. Glossary: F. Retrieved from https:// iaspub.epa.gov/sor_internet/registry/termreg/ searchandretrieve/glossariesandkeywordlists/search. do?details=&vocabName=Pesticides%20Glossary

European Commission (2009) Regulation (EC) No 1107/2009 of the European Parliament and of the Council of 21 October 2009, concerning the placing of plant protection products on the market and repealing Council Directives 79/117/EEC and 91/414/EEC. Official Journal of the European Union, L 309/1

European Commission (2013) Commission Regulation (EU) No 283/2013 of 1 March 2013 setting out the data requirements for active substances, in accordance with Regulation (EC) No 1107/2009 of the European Parliament and of the Council concerning the placing of plant protection p. Official Journal of the European Union, (Union), L 309/1

European Commission (2013) Commission Regulation (EU) No 284/2013 of 1 March 2013 setting out the data requirements for plant protection products, in accordance with Regulation (EC) No 1107/2009 of the European Parliament and of the Council concerning the placing of plant prot. Official Journal of the European Union, L 93/85

Guilherme, S., Santos, M.A., Barroso, C., Gaivão, I., Pacheco, M. (2012) Differential genotoxicity of Roundup® formulation and its constituents in blood cells of fish (Anguilla anguilla): Considerations on chemical interactions and DNA damaging mechanisms. Ecotoxicology, 21(5): 1381-1390, https://link.springer.com/article/10

Hartmann, A., Agurell, E., Beevers, C., Brendler-Schwaab, S., Burlinson, B., Clay, P., Tice, R.R. (2003) Recommendations for conducting the in vivo alkaline Comet assay. u: 4th International Comet Assay Workshop, Mutagenesis, 18(1), 45-51. Retrieved from https://www. ncbi.nlm.nih.gov/pubmed/12473734

Hauke, K., Creemers, P., Brugmans, W., van Laer, S. (2004) Signum, a new fungicide with interesting properties in resistance management of fungal diseases in strawberries. Communications in Agricultural Applied Biological Sciences, 69(4), 743-755. Retrieved from https://www. ncbi.nlm.nih.gov/pubmed/15756865

Kaur, R., Lata, M., Kaur, S. (2011) Evaluation of DNA damage in agricultural workers exposed to pesticides using single cell gel electrophoresis (comet) assay. Indian Journal of Human Genetics, 17(3): 179-179, https://www.ncbi.nlm.nih.gov/pubmed/22345990

Koller, V.J., Fürhacker, M., Nersesyan, A., Mišík, M., Eisenbauer, M., Knasmueller, S. (2012) Cytotoxic and DNA-damaging properties of glyphosate and Roundup in human-derived buccal epithelial cells. Archives of Toxicology, 86(5): 805-813

Koyama, K., Koyama, K., Goto, K. (1997) Cardiovascular effects of a herbicide containing glufosinate and a surfactant: In vitro and in vivo analyses in rats. Toxicology and Applied Pharmacology, 145(2): 409-414, https://www.sciencedirect.com/science/article/pii/S0041008X97981965?via%3Dihub

Moretti, M., Marcarelli, M., Villarini, M., Fatigoni, C., Scassellati-Sforzolini, G., Pasquini, R. (2002) In vitro testing for genotoxicity of the herbicide terbutryn: Cytogenetic and primary DNA damage. Toxicology in Vitro, 16(1): 81-88, https://www.ncbi.nlm.nih.gov/pubmed/11812643

Oakes, D.J., Pollak, J.K. (1999) Effects of a herbicide formulation, Tordon 75D®, and its individual components on the oxidative functions of mitochondria. Toxicology, 136(1): 41-52, https://www.ncbi.nlm.nih.gov/pubmed/10499849

Peixoto, F. (2005) Comparative effects of the Roundup and glyphosate on mitochondrial oxidative phosphorylation. Chemosphere, 61(8): 1115-1122

1

Ribas, G., Surralles, J., Carbonell, E., Xamena, N., Creus, A., Marcos, R. (1997) Genotoxic evaluation of the herbicide paraquat in cultured human lymphocytes. Teratogenesis, Carcinogenesis, and Mutagenesis, 17(6), 339-347. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/9485542

Santovito, A., Cervella, P., Delpero, M. (2011) In vitro aneugenic effects of the fungicide thiabendazole evaluated in human lymphocytes by the micronucleus assay. Archives of Toxicology, 85(6): 689-693

Syberg, K., Binderup, M.L., Cedergreen, N., Rank, J. (2015) Mixture Genotoxicity of 2,4-Dichlorophenoxyacetic Acid, Acrylamide, and Maleic Hydrazide on Human Caco-2 Cells Assessed with Comet Assay. Journal of Toxicology and Environmental Health, Part A, 78(6): 369-380

Undeger, U., Basaran, N. (2005) Effects of pesticides on human peripheral lymphocytes in vitro: Induction of DNA damage. Archives of Toxicology, 79(3): 169-176

Villarini, M., Moretti, M., Pasquini, R., Scassellati-Sforzolini, G., Fatigoni, C., Marcarelli, M., Monarca, S., Rodrı (1998) In vitro genotoxic effects of the insecticide deltamethrin in human peripheral blood leukocytes: DNA damage ('comet' assay) in relation to the induction of sister-chromatid exchanges and micronuclei. Toxicology, 130(2-3): 129-139

Zeljezic, D., Garaj-Vrhovac, V., Perkovic, P. (2006) Evaluation of DNA damage induced by atrazine and atrazine-based herbicide in human lymphocytes in vitro using a comet and DNA diffusion assay. Toxicology in Vitro, 20(6): 923-935
   

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/PIF1901061C
objavljen u SCIndeksu: 04.07.2019.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

Povezani članci

Gl Antropol društva Jug (1999)
Citogenetička istraživanja nekih pesticida iz grupe fenoksiherbicida
Jovičić Dubravka, i dr.

Pesticidi i fitomedicina (2019)
Herbicidi u površinskim vodama - ponašanje, efekti na akvatične organizme i procena rizika
Stevanović Marija, i dr.

Pesticidi i fitomedicina (2020)
Ocena genotoksičnog potencijala temefosa
Cobanoglu Hayal, i dr.