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2019, vol. 34, br. 3-4, str. 211-222
Karakterizacija izolata Pectobacterium carotovorum subsp. carotovorum sa kupusa izolovanih tokom skorije pojave u Bosni i Hercegovini
aInstitut za zaštitu bilja i životnu sredinu, Beograd
bUniverzitet u Beogradu, Centar za multidisciplinarne studije
cUniverzitet u Novom Sadu, Poljoprivredni fakultet

e-adresatanjaizbis@gmail.com
Projekat:
Modifikacije antioksidativnog metabolizma biljaka sa ciljem povećanja tolerancije na abiotski stres i identifikacija novih biomarkera sa primenom u remedijaciji i monitoringu degradiranih staništa (MPNTR - 43010)

Sažetak
U radu je identifikovan i okarakterisan prouzrokovač vlažne truleži kupusa u Semberiji. Simptomi su se javljali na listu u vidu lezija vlažnog izgleda uz prisustvo specifičnog mirisa. Bolest je zahvatila 20 do 30% useva. Izolacija patogena prouzrokovača bolesti je vršena na hranljivom agaru (NA), King's B i Kristal violet pektatnoj podlozi (CVP). Za dalja istraživanja odabrano je osam krem-beličastih, okruglih i ispupčenih bakterijskih kolonija koje su stvarale karakteristična udubljenja na CVP podlozi. Svi izolati su bili gram negativni, fakultativni anaerobi, oksidaza negativni, katalaza pozitivni, nefluorescentni na King-ovoj podlozi B, negativni na stvaranje levana i arginin dihidrolaze. Svi izolati su prouzrokovali vlažnu trulež na kupusu i kriškama krompira 24 časa nakon inokulacije u uslovima visoke relativne vlažnosti. Reakcija lančane reakcije polimeraze (PCR) je vršena u cilju preliminarne identifikacije izolata, primenom tri para specifičnih prajmera: F0145/E2477 (specifični za Pectobacterium carotovorum subsp. carotovorum), Br1f/L1r (specifični za P. carotovorum subsp. brasiliensis) i ECA1f/ECA2r (specifični za P. atrosepticum). Kod svih izolata amplifikovani su produkti veličine 666 baznih parova korišćenjem F0145/E2477 para prajmera, što je ukazalo na prisustvo bakterije P. carotovorum subsp. carotovorum. Dalja genetička karakterizacija je vršena na osnovu analize sekvenci konzervisanih gena gapA i mdh. BLAST analiza je potvrdila identifikaciju izolata na osnovu homologije sa sojevima P. carotovorum subsp. carotovorum deponovanim u NCBI bazi i to od 99.39% (Q. cover 100%, E. value 0.0) sa sojem M34 (Acc.no. KY047594) za sekvence gapA gena i 100% (Q. cover 100%, E. value 0.0) sa Pcc t0437 (Acc.no. KC337296) za sekvence mdh gena. Filogenetska analiza je pokazala genetičku homogenost izolata poreklom sa kupusa, svrstavajući ih u jedan klaster.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/PIF1904211P
objavljen u SCIndeksu: 08.05.2020.
metod recenzije: dvostruko anoniman
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