Metrika

  • citati u SCIndeksu: 0
  • citati u CrossRef-u:0
  • citati u Google Scholaru:[]
  • posete u poslednjih 30 dana:0
  • preuzimanja u poslednjih 30 dana:0

Sadržaj

članak: 5 od 151  
Back povratak na rezultate
2020, vol. 59, br. 4, str. 49-61
Imunohistohemijsko i morfometrijsko proučavanje gonadotropnih ćelija adenohipofize kadavera muškog pola različite starosti
Univerzitet u Nišu, Medicinski fakultet, Katedra Anatomija

e-adresajovana.cukuranovic.kokoris@medfak.ni.ac.rs
Ključne reči: luteinizirajuće gonadotropne ćelije; adenohipofiza; imunohistohemija; morfometrija; starenje
Sažetak
Podaci iz literature ukazuju na opadanje funkcije gonadotropnih ćelija sa starenjem, dok su podaci koji se odnose na strukturne promene istih oskudni. Cilj ovog rada bio je da se primenom imunohistohemijske i morfometrijske metode, detektuju i kvantifikuju promene kod humanih gonadotropnih ćelija adenohipofize kadavera muškog pola različitog uzrasta. Materijal je predstavljalo tkivo adenohipofize 14 kadavera muškog pola različite životne dobi, počev od četvrte decenije. Tkivni preseci adenohipofize standardno su histološki obrađivani i bojeni imunohistohemijski monoklonalnim anti-LH antitelom za detekciju gonadotropnih LH ćelija. Digitalne slike vidnih polja imunohistohemijski obrađenih preseka adenohipofize, zatim su morfometrijski analizirane pomoću Image J sistema. Statistička analiza vršena je pomoću SPSS statističkog paketa. Rezultati morfometrijske analize pokazali su to da se zapreminska gustina LH ćelija nije značajno menjala sa godinama, dok su njihova area, perimetar i Feretov dijametar statistički značajno rasli. Nuklearni morfometrijski parametri nisu se značajno menjali, a nukleocitoplazmatski odnos LH ćelija opadao je sa starenjem, pri čemu je taj pad bio statistički značajan kod slučajeva starijih od 70 godina. Na osnovu dobijenih rezultata, može se zaključiti da se tokom starenja gustina LH ćelija ne menja značajno, već da one hipertrofiraju, u cilju održavanja normalne sekrecije hormona. Dugotrajna hipertrofija ovih ćelija na kraju dovodi do njihovog funkcionalnog pada, koji postaje značajan nakon 70. godine života.
Reference
Allaerts, W., Salomon, B., Leenen, P.J.M., van Wijngaardt, S., Jeucken, P.H.M., Ruuls, S., Klatzmann, D., Drexhage, H.A. (1997) A population of interstitial cells in the anterior pituitary with a hematopoietic origin and a rapid turnover: A relationship with folliculo-stellate cells?. Journal of Neuroimmunology, 78(1-2): 184-97
Anđelković, Z., Somer, Lj., Avramović, V., Milosavljević, Z., Tanasković, I., Matavulj, M., et al. (2009) Histologija / Histology. Niš: Impressum, 8 th ed
Bhasin, S., Huang, G., Travison, T.G., Basaria, S. (2000-2014) Age-Related Changes in the Male Reproductive Axis. u: de Groot L.J., Chrousos G. [ur.] Endotext, South Dartmouth, MA
Console, G., Gomezdumm, C., Goya, R.L. (1994) Immunohistochemical and radioimmunological study of pituitary gonadotrophs during aging in male rats. Mechanisms of Ageing and Development, 73(2):87-95
Console, G.M., Dumm, G.C.L., Goya, R.G. (1995) Immunohistocemical and radioimmuno-logical assessment of thyrotrophs in the pituitary of aging rats. Acta Anat (Basel), 152(1):28-32
Cónsole, G.M., Gómez, D.C.L., Brown, O.A., Ferese, C.A., Goya, R.G. (1997) Sexual dimorphism in the age changes of the pituitary lactotrophs in rats. Mechanisms of Ageing and Development, 95(3):157-66
Cónsole, G.M., Jurado, S.B., Oyhenart, E., Ferese, C., Pucciarelli, H., Gómez, D.C.L.A. (2001) Morphometric and ultrastructural analysis of different pituitary cell populations in undernourished monkeys. Brazilian Journal of Medical and Biological Research, 34(1):65-74
Cónsole, G.M., Jurado, S.B., Riccillo, F.L., Gómez, D.C.L.A. (2000) Immunohistochemical and Ultrastructural Study of Pituitary Folliculostellate Cells during Aging in Rats. Cells Tissues Organs, 167(1):25-32
Console, G.M., Gomez, D.C.L.A., Goya, R.G. (1993) Impact of aging on the morphology and function of the somatotroph cell population in rats. Mechanisms of Ageing and Development, 70(1-2):45-51
Danilova, O.V., Koziritskiĭ, V.G., Gordienko, V.M., Baĭmut, F.T. (1988) Comparative ultrastructural characteristics of the anterior lobe of the hypophysis, thyroid and gonads in mammals during aging. Tsitol Genet, 22(2):11-7
Denef, C. (2008) Paracrinicity: The story of 30 years of cellular pituitary crosstalk. J Neuroendocrinol, 20(1):1-70
Feldman, H.A., Longcope, C., Derby, C.A., Johannes, C.B., Araujo, A.B., Coviello, A.D., Bremner, W.J., Mckinlay, J.B. (2002) Age Trends in the Level of Serum Testosterone and Other Hormones in Middle-Aged Men: Longitudinal Results from the Massachusetts Male Aging Study. Journal of Clinical Endocrinology & Metabolism, 87(2):589-98
Guyton, A., Hall, J.E. (2015) Textbook of Medical Physiology. Philadelphia: Elsevier Science, 13 th ed
Herman, J.P., Larson, B.R., Speert, D.B., Seasholtz, A.F. (2001) Hypothalamo-pituitary-adrenocortical dysregulation in aging F344/Brown-Norway F1 hybrid rats. Neurobiology of Aging, 22(2):323-32
Jurado, S., Cónsole, G., Gomez, D.C. (1998) Sexually Dimorphic Effects of Aging on Rat Somatotroph Cells: An Immunohistochemical and Ultrastructural Study. Journal of Veterinary Medical Science, 60(6):705-11
Keenan, D.M., Licinio, J., Veldhuis, J.D. (2001) A feedback-controlled ensemble model of the stress-responsive hypothalamo-pituitary-adrenal axis. Proceedings of the National Academy of Sciences, 98(7):4028-33
Kurosumi, K., Ozawa, H., Akiyama, K., Senshu, T. (1991) Immunoelectron Microscopic Studies of Gonadotrophs in the Male and Female Rat Anterior Pituitaries, with Special Reference to their Changes with Aging. Archives of Histology and Cytology, 54:559-71
Medigović, I., Manojlović-Stojanoski, M., Trifunović, S., Ristić, N., Milošević, V., Žikić, D., Nestorović, N. (2012) Effects of genistein on gonadotropic cells in immature female rats. Acta Histochemica, 114(3):270-5
Meeran, D., Urbanski, H.F., Gregory, S.J., Townsend, J., Tortonese, D.J. (2003) Developmental Changes in the Hormonal Identity of Gonadotroph Cells in the Rhesus Monkey Pituitary Gland. Journal of Clinical Endocrinology & Metabolism, 88(6): 2934-42
Mills, S.E. (2007) Histology for pathologists. Philadelphia: Lippincott Williams and Wilkins, 3 rd ed
Mitchell, R.S., Kumar, V., Abbas, A.K., Fausto, N. (2007) Basic Pathology. u: Kumar V., Abbas A.K., Fausto N., Mitchell R.N. [ur.] Robbins Basic Pathology, Philadelphia: Saunders, 8 th ed
Mulligan, T., Iranmanesh, A., Kerzner, R., Demers, L.W., Veldhuis, J.D. (1999) Two-week pulsatile gonadotropin releasing hormone infusion unmasks dual (hypothalamic and Leydig cell) defects in the healthy aging male gonadotropic axis. European Journal of Endocrinology, 141(3): 257-66
Perez-Castro, C., Renner, U., Haedo, M.R., Stalla, G.K., Arzt, E. (2012) Cellular and Molecular Specificity of Pituitary Gland Physiology. Physiological Reviews, 92(1):1-38
Rudman, D., Feller, A.G., Nagraj, H.S., Gergans, G.A., Lalitha, P.Y., Goldberg, A.F., Schlenker, R.A., Cohn, L., Rudman, I.W., Mattson, D.E. (1990) Effects of Human Growth Hormone in Men over 60 Years Old. New England Journal of Medicine, 323(1):1-6
Russ, J.C., Dehoff, R.T. (2000) Practical Stereology. New York: Kluwer Academic-Plenum Publishers, 2 nd ed
Sasaki, F. (1988) Changes with age in the number and size of anterior pituitary cells in female mice from suckling to adulthood. Journal of Endocrinology, 117(1):5-10
Schwartz, J., Pavert, S., Clarke, I., Rao, A., Ray, D.I., Vrana, K. (1998) Paracrine Interactions within the Pituitary Gland. Annals of the New York Academy of Sciences, 839:239-43
Smith, R.G., Betancourt, L., Sun, Y. (2005) Molecular Endocrinology and Physiology of the Aging Central Nervous System. Endocrine Reviews, 26(2):203-50
Sternberger, L.A., Hardy, P.H.Jr., Cuculis, J.J., Meyer, H.G. (1970) The unlabeled antibody enzyme method of immunehistochemistry: Preparation and properties of soluble antigen-antibody complex (horseradish peroxidaseantihorseradish peroxidase) and its use in identification of spirochetes. Journal of Histochemistry & Cytochemistry, 18(5):315-33
Strauss, J.F., Barbieri, R.L., Snyder, P.J. (2009) Male Reproductive Aging. u: Yen & Jaffe's Reproductive Endocrinology, Philadelphia: Elsevier, p. 357-63
Takahashi, S., Kawashima, S. (1983) Age-related changes in prolactin cells in male and female rats of the Wistar/Tw strain. J Sci Hiroshima Univ, 31: 185-91
van den Beld, A.W., Lamberts, S.W.J. (2002) Endocrine Aspects of Healthy Ageing in Men. Novartis Found Symp, 242: 3-25
Veldhuis, J.D. (2008) Aging and hormones of the hypothalamo-pituitary axis: Gonadotropic axis in men and somatotropic axes in men and women. Ageing Research Reviews, 7(3):189-208
Veljković, S., Radenković, M., eds. (2016) Medicinska fiziologija. Niš: Medicinski fakultet Univerziteta u Nišu
Weiss, J., Lansing, A.I. (1953) Age Changes in the Fine Structure of Anterior Pituitary of the Mouse. Proc Soc Exp Biol Med, 82(3):460-6
Young, B., Lowe, J.S., Stevens, A., Heath, J.W. (2007) Wheater's Functional Histology: A Text and Colour Atlas. Philadelphia: Elsevier Churcill Livinigstone, 5 th ed
 

O članku

jezik rada: engleski
vrsta rada: originalan članak
DOI: 10.5633/amm.2020.0407
objavljen u SCIndeksu: 05.02.2021.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

Povezani članci