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2017, vol. 74, br. 8, str. 736-741
Određivanje referentnih vrednosti aktivnosti acetil i butiril holinesteraze kod zdrave populacije u Srbiji
aVojnomedicinska akademija, Nacionalni centar za kontrolu trovanja, Beograd + Univerzitet odbrane, Medicinski fakultet Vojnomedicinske akademije, Beograd
bVojnomedicinska akademija, Nacionalni centar za kontrolu trovanja, Beograd
cVojnomedicinska akademija, Institut za medicinsku biohemiju, Beograd

e-adresazmilica5@gmail.com
Sažetak
Uvod/Cilj. Acetilholinesteraza (AChE) i butirilholinesteraza (BuChe) su važni biomarkeri ekspozicije organofosfornim i karbamatnim insekticidima. S obzirom na to da procena nivoa inhibicije holinesteraze, koja odgovara težini trovanja ovim agensima, zavisi od normalnih vrednosti, koje mogu varirati u različitim populacijama, bilo je važno odrediti ih u našoj populaciji, što do sada nije urađeno. Stoga je cilj ovog rada bio da se odrede referentne vrednosti za aktivnost AChE i BuChE u zdravoj populaciji odraslih osoba na području Republike Srbije. Metode. Aktivnost AChE u eritrocitima merena je spekrofotometrijski (λ = 412 nm). Korišćena je modifikovana Ellman-ova metoda. Aktivnost BuChE u plazmi određivana je na integrisanom hemijskom sistemu (Dimenzion RxLMax) sa gotovim reagens uloškom za analizu. Ispitanici su bili dobrovoljni davaoci krvi iz Instituta za transfuziologiju i hemobiologiju Vojnomedicinske akademije. Za obradu podataka korišćen je SPSS (Statistical Package for Social Sciences) softverski program. Rezultati. U grupi od 851 osobe bilo je 728 muškaraca i 123 žene. Srednje životno doba ispitanika bilo je 39,1 ± 11,6 godina. Za sve ispitanike određena je aktivnost AChE, dok je aktivnost butirilholinesteraze određena kod 205 ispitanika (169 muškaraca i 36 žena). Srednja vrednost aktivnosti acetilholinesteraze iznosila je 8 090,6 ± 1 976,7 IJ/L, a za butirilholinesterazu 14 556.6 ± 4 078,1 U/L. Odsustvo normalne distribucije podataka kod muškaraca uslovilo je da referentne vrednosti enzima odredimo kao 2,5-ti i 97,5- ti percentil. Zaključak. Rezultati ove pilot studije o aktivnosti holinesteraza u zdravoj populaciji u Republici Srbiji, koja je sada urađena prvi put, ukazuju na potrebu da se pri proceni težine trovanja uzmu u obzir širi rasponi aktivnosti holinesteraza. Preporučuje se proširivanje studije za BuChE, sa uključenjem većeg broja ispitanika ženskog pola i podataka o telesnoj masi, u cilju dobijanja preciznijih referentnih vrednosti.
Reference
*** Test name (Cholinesterase, Serum, Plasma, RBC). U.S: Quest Diagnostics clinical laboratory, Available from: http://www.questdiagnostics.com/testcenter/TestDetail.action?ntc=39481
Abdullat, E.M., Obeidat, N.M., Al-Jaghbir, M.T. (2015) Establishing the Cholinesterase Levels in Jordan : An Investigation and Monitoring Model for Pesticides Outbreak = تحديد مستوى الكولين أستريز في الأردن : نموذج تقصي و رصد لحدوث انتشار التسمم بالمبيدات. Jordan Medical Journal, 49(3): 129-138
Aldridge, W. N., Davies, D. R. (1952) Determination of Cholinesterase Activity in Human Blood. BMJ, 1(4765): 945-947
Bosak, A., Katalinić, M., Kovarik, Z. (2011) Cholinesterases: Structure, Role, and Inhibition. Archives of Industrial Hygiene and Toxicology, 62(2):
Brock, A., Brock, V. (1993) Factors affecting inter-individual variation in human plasma cholinesterase activity: Body weight, height, sex, genetic polymorphism and age. Archives of Environmental Contamination and Toxicology, 24(1): 93-99
Brock, A., Brock, V. (1990) Plasma cholinesterase activity in a healthy population group with no occupational exposure to known cholinesterase inhibitors: relative influence of some factors related to normal inter- and intra-individual variations. Scandinavian Journal of Clinical and Laboratory Investigation, 50(4): 401-408
Bruns, D.E., Ashwood, E.R., Burtis, C.A. (2012) Clinical Chemistry, Molecular Diagnostics, and Laboratory Medicine. u: Tietz Textbook of Clinical Chemistry and Molecular Diagnostics, Elsevier BV, str. 3-6
Chan, L., Balabaskaran, S., Delilkan, A.E., Ong, L.H. (1994) Blood cholinesterase levels in a group of Malaysian blood donors. Malays J Pathol, 16(2): 161-4
Chen, X., Fang, L., Liu, J., Zhan, C. (2011) Reaction Pathway and Free Energy Profile for Butyrylcholinesterase-Catalyzed Hydrolysis of Acetylcholine. Journal of Physical Chemistry B, 115(5): 1315-1322
Christenson, W., Vangoethem, D., Schroeder, R., Wahle, B., Dass, P., Sangha, G., Thyssen, J. (1994) Interlaboratory cholinesterase determinations and the effect on the results of statistical evaluation of cholinesterase inhibition☆. Toxicology Letters, 71(2): 139-150
Colovic, M.B., Krstic, D.Z., Lazarevic-Pasti, T.D., Bondzic, A.M., Vasic, V.M. (2013) Acetylcholinesterase Inhibitors: Pharmacology and Toxicology. Current Neuropharmacology, 11(3): 315-335
Davis, L., Britten, J. J., Morgan, M. (1997) Cholinesterase Its significance in anaesthetic practice. Anaesthesia, 52(3): 244-260
Ellman, G.L., Courtney, K., Andres, V., Featherstone, R.M. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochemical Pharmacology, 7(2): 88-95
Gal, E.M., Roth, E. (1957) Spectrophotometric methods for determination of cholinesterase activity. Clinica Chimica Acta, 2(4): 316-326
Guidi, G.C., Salvagno, G.L. (2010) Reference intervals as a tool for total quality management. Biochemia Medica, str. 165-172
Harris, E.K., Boyd, J.C. (1990) On dividing reference data in to subgroups to produce separate reference ranges. Clin Chem, 36(2): 265-70
Holmstedt, B. (2000) Cholinesterase inhibitors: An introduction. u: Giacobini E. [ur.] Cholinesterases and cholinesterase inhibitors, London: Martin Dunitz Ltd, p. 1-8
Horowitz, G.L., Altaie, C.S., Boyd, J.C., Ceriotti, F., Garg, U., Horn, P. (2008) Defining, establishing, and verifying reference intervals in the clinical laboratory: Approved guideline. Wayne, PA: CLSI, 3rd ed
Johnell, K., Fastbom, J. (2008) Concurrent Use of Anticholinergic Drugs and Cholinesterase Inhibitors. Drugs & Aging, 25(10): 871-877
Karalliedde, L., Feldman, S., Henry, J., Marrs, T., Karalliedde, L., Henry, J. (2011) The Acute Cholinergic Syndrome. u: Organophosphates and Health, World Scientific Pub Co Pte Lt, str. 109-140
Lepage, L., Schiele, F., Gueguen, R., Slest, G. (1985) Total cholinesterase in plasma: Biological variations and reference limits. Clin Chem, 31(4): 546-550
Pohanka, M. (2011) Cholinesterases, a target of pharmacology and toxicology. Biomedical Papers, 155(3): 219-223
Reiner, E., Šinko, G., Škrinjarić-Špoljar, M., Simeon-Rudolf, V. (2000) Comparison of protocols for measuring activities of human blood cholinesterases by the Ellman method. Arch Hig Rada Toksikol, 51: 13-8
Sánchez, L.H., Medina, O.M., Gómez, G., González, C.I., Flórez-Vargas, Ó. (2015) Laboratory genetic-based reference values for cholinesterase activity in a Colombian population: A step forward in personalized diagnostics. Biomedica, 35 Spec: 20−9
Santarpia, L., Grandone, I., Contaldo, F., Pasanisi, F. (2012) Butyrylcholinesterase as a prognostic marker: a review of the literature. Journal of Cachexia, Sarcopenia and Muscle, 4(1): 31-39
Solberg, H.E. (2004) The IFCC recommendation on estimation of reference intervals. The RefVal Program. Clinical Chemistry and Laboratory Medicine (CCLM), 42(7):
Soliday, F.K., Conley, Y.P., Henker, R. (2010) Pseudocholinesterase deficiency: A comprehensive review of genetic, acquired, and drug influences. AANA J, 78(4): 313-20
Sunderman, F.W.Jr. (1975) Current concepts of normal values, reference values, and discrimination values, in clinical chemistry. Clin Chem, 21(13): 1873−7
Trundle, D., Marcial, G. (1988) Detection of Cholinesterase inhibition: The significance of Cholinesterase measurements. Ann Clin Lab Sci, 18(5): 345-52
U.S. Mayo Clinic-Mayo Medical Laboratories Pseudocholinesterase, total, serum. Available from: http://www.mayomedicallaboratories.com/testcatalog/Clinical+and+Interpretive/8l+and+Interpretive/8
Westgard, J.O., Hund, M.R., Lahmeyer, B.L. (2003) Estimates of normal ranges for 15 determinations on the du pont pont automatic clinical analyzer. Madison, WI: University of Wisconsin-Center for Health Science-Dept. of Medicine and Clinical Laboratories
Whittaker, V.P. (2010) How the cholinesterases got their modern names. Chemico-Biological Interactions, 187(1-3): 23-26
Worek, F., Mast, U., Kiderlen, D., Diepold, C., Eyer, P. (1999) Improved determination of acetylcholinesterase activity in human whole blood. Clinica Chimica Acta, 288(1-2): 73-90
 

O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/VSP160303101Z
objavljen u SCIndeksu: 04.08.2017.
metod recenzije: dvostruko anoniman
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