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2017, vol. 74, iss. 8, pp. 736-741
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Determination of reference values of acetyl and butyryl cholinesterase activities in Serbian healthy population
Određivanje referentnih vrednosti aktivnosti acetil i butiril holinesteraze kod zdrave populacije u Srbiji
aMilitary Medical Academy, National Poison Control Center, Belgrade + University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade bMilitary Medical Academy, National Poison Control Center, Belgrade cMilitary Medical Academy, Institute of Medical Biochemistry, Belgrade
email: zmilica5@gmail.com
Abstract
Background/Aim. Acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) are important biomarkers of exposure to organophosphorus and carbamate insecticides. Since the estimation of the level of cholinesterase inhibition depends on the normal values which may vary in different populations, it is important to determine them in our population, which so far has not been done. Therefore, the aim of this study was to determine the reference values for AChE and BuChE in a healthy population of adults in the Republic of Serbia. Methods. The AChE activity was measured by spectrophotometry (λ = 412 nm), using a modified Ellman’s method. BuChE activity was determined by the integrated chemical system (Dimension RxLMax) with ready-made reagent cartridge for analysis. The examinees were healthy voluntary blood donors from the Institute of Transfusiology and Hemobiology, Military Medical Academy in Belgrade, Serbia. Statistical Package for Social Sciences (SPSS) software program was used for data processing. Results. In the group of 851 persons, there were 728 males and 123 females. The mean age was 39.1 ± 11.6 years. For all of them, erythrocyte AChE activity was done while BuChE was determined in 205 persons (169 males and 36 females). Their mean value of acetylcholinesterase activity was 8,090.6 ± 1,976.7 IU/L, and of butyrylcholinesterase activity was 14,556.6 ± 4,078.1 U/L. Due to lack of normal data distribution in male group (both enzymes), reference ranges were estimated as 2.5 and 97.5 percentiles. Conclusion. The results of this pilot study on cholinesterase in healthy population in the Republic of Serbia which has now been done for the first time, indicate the need for considering their wider ranges of when estimating the severity of poisoning. However, further study for BuChE with the inclusion of a larger number of females and data for body weight of the examinees, in order to get more precise reference limits, is suggested.
Sažetak
Uvod/Cilj. Acetilholinesteraza (AChE) i butirilholinesteraza (BuChe) su važni biomarkeri ekspozicije organofosfornim i karbamatnim insekticidima. S obzirom na to da procena nivoa inhibicije holinesteraze, koja odgovara težini trovanja ovim agensima, zavisi od normalnih vrednosti, koje mogu varirati u različitim populacijama, bilo je važno odrediti ih u našoj populaciji, što do sada nije urađeno. Stoga je cilj ovog rada bio da se odrede referentne vrednosti za aktivnost AChE i BuChE u zdravoj populaciji odraslih osoba na području Republike Srbije. Metode. Aktivnost AChE u eritrocitima merena je spekrofotometrijski (λ = 412 nm). Korišćena je modifikovana Ellman-ova metoda. Aktivnost BuChE u plazmi određivana je na integrisanom hemijskom sistemu (Dimenzion RxLMax) sa gotovim reagens uloškom za analizu. Ispitanici su bili dobrovoljni davaoci krvi iz Instituta za transfuziologiju i hemobiologiju Vojnomedicinske akademije. Za obradu podataka korišćen je SPSS (Statistical Package for Social Sciences) softverski program. Rezultati. U grupi od 851 osobe bilo je 728 muškaraca i 123 žene. Srednje životno doba ispitanika bilo je 39,1 ± 11,6 godina. Za sve ispitanike određena je aktivnost AChE, dok je aktivnost butirilholinesteraze određena kod 205 ispitanika (169 muškaraca i 36 žena). Srednja vrednost aktivnosti acetilholinesteraze iznosila je 8 090,6 ± 1 976,7 IJ/L, a za butirilholinesterazu 14 556.6 ± 4 078,1 U/L. Odsustvo normalne distribucije podataka kod muškaraca uslovilo je da referentne vrednosti enzima odredimo kao 2,5-ti i 97,5- ti percentil. Zaključak. Rezultati ove pilot studije o aktivnosti holinesteraza u zdravoj populaciji u Republici Srbiji, koja je sada urađena prvi put, ukazuju na potrebu da se pri proceni težine trovanja uzmu u obzir širi rasponi aktivnosti holinesteraza. Preporučuje se proširivanje studije za BuChE, sa uključenjem većeg broja ispitanika ženskog pola i podataka o telesnoj masi, u cilju dobijanja preciznijih referentnih vrednosti.
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