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2019, vol. 58, iss. 2, pp. 135-144
Effect of curcumin and p53 signaling pathway in rat thymocytes toxicity induced by Mancozeb
aUniveristy of Niš, Faculty of Medicine, Department of Physiology
bClinical Centre Niš, Clinic of Eye Diseases

emailvojapav@yahoo.com
Abstract
Mancozeb, as a dithiocarbamate fungicide, is widely used in agriculture due to its low acute toxicity and short environmental persistence. We examined the protective role of curcumin on Mancozeb-induced toxicity in rat thymocytes and potential mechanisms involved. Rat thymocytes were exposed to Mancozeb (0.01 μg/ml) and/or curcumin (0.3, 1, 3 μM) and levels of cell viability, caspase-3, caspase-9 activity, cytochrome C oxidase, catalase activity, reactive oxygen species (ROS) production and p53 signaling involvement were evaluated after 24 h of incubation. Cells treated with Mancozeb showed increased toxicity, caspase-3, 9 activity and ROS production with decreased cytochrome C oxidase and catalase activity. Inhibition of caspase-3 and 9 activity resulted with reduced rat thymocytes toxicity while inhibition of p53 signaling pathway suppressed caspase-3 activity in cells. Co-treatment with curcumin (1, 3 μM) displayed significantly reduced toxicity, caspase-3, 9 activity and ROS production, together with increased cytochrome C and catalase activity in cells. These findings propose that Mancozeb-induced apoptosis in rat thymocytes is caspase dependent and is partially attributed to p53 signaling pathway. Certain curcumin concentrations may modulate Mancozeb-induced rat thymocytes toxicity, due to its anti-oxidative effect, and may be useful for providing potential therapeutic strategy in immunomodulation induced by Mancozeb.
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About

article language: English
document type: Original Scientific Paper
DOI: 10.5633/amm.2019.0221
published in SCIndeks: 09/09/2019
peer review method: double-blind
Creative Commons License 4.0

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