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2015, vol. 54, br. 4, str. 5-11
Efekat kvercentina na mitohondrije timocita pacova tretiranih Mankozebom
Univerzitet u Nišu, Medicinski fakultet, Katedra Fiziologija

e-adresavojapav@yahoo.com
Ključne reči: Mankozeb; toksičnost; timociti; kvercetin; mitohondrija
Sažetak
Kvercetin je jedan od najrasprostranjenijih flavonoida koji se putem hrane kontinuirano unosi u ljudski organizam. Ovaj flavonoid poseduje snažno antioksidativno i antiinflamacijsko dejstvo. U studiji je evaluiran efekat Mankozeba (0,2, 2 i 5 µg/ml), Makozeba i kvercetina, samo kvercetina (10 mM) na vijabilnost, apoptozu, produkciju slobodnih kiseoničkih radikala (ROS) i mitohondrijalnog membranskog potencijala (MMP) u timocitima pacova u in vitro uslovima. Tretman ćelija sa Mankozebom doveo je do dozno zavisnog smanjenja vijabilnosti ćelija, indukcije apoptoze koja je praćena povećanom sintezom ROS-a i smanjenjem MMP-a. Aplikacija kvercetina značajno je smanjila citotoksičnost u kulturama sa 0,2 i 2 µg/ml Mankozeba, zajedno sa smanjenjem ROS produkcije i povećanjem MMP-a. Kvercetin u ćelijama tretiranim sa 5 µg/ml Mankozeba nije uspeo da redukuje toksičnost, već je indukovao povećanje broja apoptotičnih ćelija. Dobijeni rezultati pokazuju da produkcija ROS-a, zajedno sa mitohondrijalnom disfunkcijom, predstavlja značajan faktor u toksičnosti indukovanoj Mankozebom. Administracija kvercetina redukuje toksičnost ćelija koja je indukovana nižim koncentracijama Mankozeba, sa mogućom sposobnošću da indukuje apoptozu i spreči nekrozu ćelija, a samim tim i razvoj sekundarnih imunoloških konsekvenci.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5633/amm.2015.0401
objavljen u SCIndeksu: 27.04.2016.

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