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2017, vol. 54, br. 2, str. 73-78
Povezanost disaharidnih profila odredenih GC/EI-qMS sistemom sa genomima sorti pšenice, raži i tritikalea
aUniverzitet u Novom Sadu, Tehnološki fakultet
bUniverzitet u Novom Sadu
cNaučni institut za ratarstvo i povrtarstvo, Novi Sad

e-adresapastor@tf.uns.ac.rs
Projekat:
Savremeno oplemenjivanje strnih žita za sadašnje i buduće potrebe (MPNTR - 31066)
COST Action FA1306: The quest for tolerant varieties: Phenotyping atplant and cellular level

Ključne reči: Triticum aestivum; Secale cereale; Triticosecale; TMSO derivati; GC/EI-qMS; disaharidi; multivarijantna analiza
Sažetak
Sorte strnih žita: pšenice (Triticum aestivum), raži (Secale cereale) i tritikalea (Triticosecale), analizirane su primenom sistema gasne hromatografije sa maseno-spektrometrijskom detekcijom zbog specifičnog biohemijskog sastava ugljenih hidrata. Uzorci su prvo samleveni u brašno, a potom ekstrahovani 96%-tnim rastvorom etanola. Ekstrahovani disaharidi su zatim derivatizovani u odgovarajuće trimetilsilil-oksime i analizirani pomoću GC/EI-qMS uređaja. Bibliotečkim pretraživanjem Wiley 275 i NIST 14 baza masenih spektara četiri eluirajuća pika, sa specifičnim vremenima retencije (18,57 min, 18,80 min, 20,85 min, i 21,39 min), identifikovana su kao trimetilsilil-oksimi turanoze, melibioze i saharoze, i odabrana kao diskriminirajući faktori. Njihovo prisustvo, odnosno, odsustvo u određenim sortama strnih žita označeno je sa '1', odnosno, '0', kreirajući pritom binarne numeričke matrice, koje su podvrgnute multivarijantnoj analizi. Primenjene istraživačke tehnike analize podataka pružile su dobru osnovu za diferencijaciju pšenice, raži i tritikalea, bez obzira na specifičnu sortu kojoj pripadaju, izbegavajući pritom kvantitativnu analizu definisanih markera diskriminacije. Pretpostavlja se da uočena izraženija sličnost između sorti pšenice i tritikalea, u odnosu na raž, može biti rezultat činjenice da najveći deo strukture genoma sorti tritikalea vodi poreklo upravo od sorti pšenice.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/ratpov54-13405
objavljen u SCIndeksu: 02.10.2017.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

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