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2021, vol. 58, iss. 1, pp. 1-6
Effect of different carbon and nitrogen sources combination in medium for production of biocontrol agent Trichoderma harzianum
aUniversity of Novi Sad, Faculty of Technology, Department of Biotechnology and Pharmaceutical Engineering
bInstitute of Field and Vegetable Crops, Novi Sad

emailtadi@uns.ac.rs
Project:
The study is the result of the investigations conducted within the project 142-451-3213/2020-03 funded by Provincial Secretariat for Higher Education and Scientific Research, Autonomous Province of Vojvodina, Republic of Serbia.

Keywords: Aspergillus flavus; biocontrol; carbon; dextrose; Fusarium graminearum; medium; nitrogen; soybean flour; Trichoderma harzianum; well diffusion method
Abstract
The increasing usage of chemicals for plant protection in recent years has become a serious problem. One of the possible solutions is use of beneficial microorganisms instead of synthetic fungicides, which will contribute to the protection of the environment and human health. Since the fungi Aspergillus flavus and Fusarium graminearum are the most important pathogens that cause maize diseases and produce mycotoxins, the potential of Trichoderma harzianum for biocontrol of both phytopathogens was examined in this paper. The aim of this paper was to study the influence of different carbon and nitrogen combinations in the medium for T. harzianum production. T. harzianum was cultivated in Erlenmeyer flasks and the effect of cultivation broth against selected maize pathogens was tested using well diffusion method. The results of this study showed that the combination of different carbon and nitrogen sources in the T. harzianum cultivation medium statistically significantly affects the production of Trichoderma cultivation broth effective on two tested phytopathogens. Dextrose as a carbon source and soybean flour as a nitrogen source proved to be the best combination in the medium for production of T. harzianum cultivation broth effective on A. flavus and F. graminearum. Maximal inhibition zone diameters of 31 mm and 56.33 mm were registered in those medium formulations for A. flavus and F. graminearum, respectively. These researches represent an important step for further research in which a medium of low market value would be selected. This would reduce the price of the production process but also the final product.
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About

article language: English
document type: Original Scientific Paper
DOI: 10.5937/ratpov58-29961
received: 21/12/2020
accepted: 22/01/2021
published in SCIndeks: 29/04/2021
peer review method: double-blind
Creative Commons License 4.0

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