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2012, vol. 49, br. 1, str. 34-38
Detekcija Xanthomonas axonopodis pv. phaseoli i Pseudomonas savastanoi pv. phaseolicola sa semena pasulja korišćenjem Milk-tween podloge
aInstitut za zaštitu bilja i životnu sredinu, Beograd
bNaučni institut za ratarstvo i povrtarstvo, Novi Sad
cInstitut za zemljište, Beograd

e-adresatanjaizbis@gmail.com
Sažetak
Proizvodnju pasulja ugrožavaju fitopatogene bakterije prouzrokovači plamenjača Xanthomonas axonopodis pv. phaseoli (Xap) i Pseudomonas savastanoi pv. phaseolicola (Psp). S obzirom da do sada nije postignuta zadovoljavajuća hemijska zaštita za suzbijanje ovih bakterija, mere koje se preporučuju preventivnog su karaktera i uključuju upotrebu zdravog semena za setvu, plodored, duboko zaoravanje ostataka i korišćenje otpornih sorti. U ovom radu je vršena detekcija Xap i Psp na semenu pasulja metodom izolacije na poluselektivnu podlogu Milk Tween Agar (MT). Na ovoj podlozi Xap obrazuje žute, sluzaste i ispupčene kolonije sa dve zone hidrolize (manjim mlečnim i većim prosvetljenim), a Psp obrazuje kolonije beličasto-krem, ravne i okrugle. Identifikacija izolata Xap i Psp je potvrđena primenom ELISA i PCR. Podloga MT se zbog svoje selektivnosti, jednostavne pripreme i mogućnosti istovremene detekcije bakterija Xap i Psp može preporučiti prilikom rutinskog ispitivanja zdravstvenog stanja semena pasulja za setvu proizvedenog kod nas ili prilikom kontrole semena iz uvoza.
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O članku

jezik rada: srpski
vrsta rada: izvorni naučni članak
DOI: 10.5937/ratpov49-1126
objavljen u SCIndeksu: 12.04.2012.
metod recenzije: dvostruko anoniman