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2016, vol. 53, iss. 2, pp. 42-45
Occurrence, identification and phylogenetic analysis of Fusarium proliferatum on bean seed (Phaseolus vulgaris L.) in Serbia
aInstitute of Field and Vegetable Crops, Novi Sad
bInstitute for Plant Protection and Environment, Belgrade

emailmaja.ignjatov@ifvcns.ns.ac.rs
Project:
Development of vegetable cultivars and hybrids intended for outdoor and indoor production (MESTD - 31030)

Abstract
During the routine quality control analysis on bean seeds in 2015, Fusarium fungal infection was observed on an average of 17% of the bean seed. The objective of this paper was isolation and identification of Fusarium sp. based on the pathogen's morphological and molecular characteristics. Morphological identification of Fusarium isolates was performed on PDA and CLA. DNA of 14 Fusarium sp. isolates was extracted directly from the mycelium (~ 100 mg wet weight), with a Dneasy Plant Mini Kit (Qiagen, Hilden, Germany). Following DNA extraction, the translation elongation factor 1-alpha region was amplified by PCR with the primer pair EF1 and EF2. An amplicon of 700 bp was amplified in all tested isolates. Identification of one isolate was performed by sequencing the translation elongation factor EF-1a gene. Completed morphological and molecular characteristics of isolates, as well as the results of sequencing confirmed that Fusarium proliferatum was the causal agent of bean seed rot.
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About

article language: English
document type: Original Scientific Paper
DOI: 10.5937/ratpov53-9984
published in SCIndeks: 01/09/2016
peer review method: double-blind
Creative Commons License 4.0