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2020, vol. 39, iss. 4, pp. 428-435
Association of the sEH gene promoter polymorphisms and haplotypes with preeclampsia
aNiğde Omer Halisdemir University, Faculty of Medicine, Department of Medical Biochemistry, Niğde, Turkey
bBeykent University, Faculty of Medicine, Department of Medical Biochemistry, Istanbul, Turkey
cBeykent University, Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey
dNiğde Omer Halisdemir University, Faculty of Medicine, Department of Obstetrics and Gynaecology, Niğde, Turkey

emailsmlsr@hotmail.com
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This work was supported by the Scientific Research Project Fund of Sivas Cumhuriyet University under project number T-658.

Keywords: epoxyeicosatrienoic acids; preeclampsia; soluble epoxide hydrolase; polymorphism; gene promoter
Abstract
Background: The epoxyeicosatrienoic acids (EETs) have antihypertensive, anti-inflammatory, and organ protective properties and their circulation levels are related to hypertension, diabetes mellitus, cardiovascular diseases, and preeclampsia. Soluble epoxide hydrolase (sEH) catalyses the degradation of EETs to less biologically active dihydroxyeicosatrienoic acids. Here, we sequenced the promoter region of EPHX2 to investigate the association between promoter sequence alterations that we thought to affect the expression levels of the enzyme and preeclampsia (PE). Methods: Nucleotide sequencing of the promoter region of the EPHX2, spanning from position -671 to +30, was performed on 100 pregnant women with PE and, 20 or more weeks pregnant normotensive, healthy women (n=100). Results: Pregnant women who carry rs4149235, rs4149232, rs73227309, and rs62504268 polymorphisms have 4.4, 2.4, 2.3, and 2.8 times significantly increased risk of PE, respectively. CCGG (OR: 3.11; 95% CI: 1.12-8.62) and CCCA (OR: 0.45; 95% CI: 0.36-0.55) haplotypes were associated with an increased and decreased risk of PE, respectively. Conclusions: Four SNPs (rs4149232, rs4149235, rs73227309, and rs62504268) in the promoter region of the EPHX2, and CCGG and CCCA haplotypes of these 4 SNPs were significantly associated with PE. These SNPs in the promoter region may affect sEH expression and thus enzyme activity and may play a role in PE pathogenesis by causing individual differences in EET levels. However, future studies are needed to confirm our findings and examine the effect of these SNPs on the sEH expression and/or enzyme activity.
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article language: English
document type: Original Scientific Paper
PMC ID: PMC7710375
DOI: 10.5937/jomb0-27745
published in SCIndeks: 02/10/2020
peer review method: double-blind
Creative Commons License 4.0

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