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2020, vol. 65, br. 4, str. 375-389
Genetička karakterizacija gajenih i divljih populacija afričkog soma (Clarias gariepinus burchell, 1822) primenom RAPD metoda
University of Maiduguri, Faculty of Agriculture, Department of Fisheries, Maiduguri, Nigeria

e-adresasaadbala@unimaid.edu.ng
Ključne reči: RAPD; genetička varijacija; Clarias gariepinus; polimorfizam; Nigerija; marker
Sažetak
U ovom istraživanju sprovedena je genetička karakterizacija afričkog soma Clarias gariepinus. Prikupljeno je trideset (30) uzoraka afričkog soma C. gariepinus, po petnaest (15) iz divljine i sa ribnjaka u severnoistočnoj Nigeriji zbog njihove genetičke srodnosti i divergentnosti uz pomoć markera RAPD metode. Ekstrakcija DNK iz uzorka krvi izvršena je pomoću kompleta hemikalija Gene Jet Genomic DNA Purification Kit. Pet prajmera je korišćeno za PCR reakciju i amplifikovane su 402 RAPD trake poreklom iz četiri soja Clarias gariepinus. Trista šest (76,12%) traka bilo je polimorfno, dok je 86 (21,39%) bilo monomorfno. Procenat polimorfizma dobijen kod uzgajanih, odnosno divljih populacija, kretao se od 58 (47,3%) do 69 (75,9%). Polimorfne trake po lokusima unutar populacija kretale su se od 67,9% do 82,0%. Prosečne vrednosti koeficijenta inbridinga (FIS) iznosili su 0,083 kod uzgajane odnosno 0,053 kod divlje populacije. Vrednosti divergentnosti gena unutar uzgajanih i divljih populacija (Ht) bile su 0,4522 odnosno 0,4018. Srednja genetička diferencijacija (FST) kretala se između (0,203) kod uzgajanih i 0,129 kod divljih populacija. Analiza molekularne varijanse pokazala je da je unutar populacije bilo 96% genetičke varijanse, a među populacijom 4%. Genetički identitet i genetička distanca četiri populacije iznosili su 0,9490 odnosno 0,1038. Filogenetska mera pokazala je da su četiri soja podeljena u dva klastera na nivou sličnosti od približno 0,089. Rezultati su ukazali na značajan nivo genetičkih varijacija i minimalno odvajanje dendrograma kod Clarias gariepinus na severoistoku Nigerije. U zaključku, ove informacije će biti korisno sredstvo za program selekcije afričkog soma Clarias gariepinus.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/JAS2004375S
primljen: 05.03.2019.
prihvaćen: 20.08.2020.
objavljen u SCIndeksu: 21.01.2021.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

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