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2020, vol. 39, iss. 1, pp. 91-99
NLRP3 and CARD8 polymorphisms influence risk for asbestos-related diseases
aUniversity Medical Centre Ljubljana, Clinical Institute of Occupational Medicine, Ljubljana, Slovenia
bUniversity of Ljubljana, Faculty of Medicine, Institute of Biochemistry, Pharmacogenetics Laboratory, Ljubljana, Slovenia
cInstitute of Oncology Ljubljana, Ljubljana, Slovenia
This work was financially supported by the Slovenian Research Agency (ARRS Grants No. P1-0170, L3-8203)

Keywords: inflammasome; polymorphism; asbestosis; pleural plaques; malignant mesothelioma
Background: This study aimed to investigate the association between NLRP3 rs35829419 and CARD8 rs2043211 polymorphisms and the risk of developing pleural plaques, asbestosis, and malignant mesothelioma (MM), and to study the influence of the interactions between polymorphisms and asbestos exposure on the risk of developing these diseases. Methods: The case-control study included 416 subjects with pleural plaques, 160 patients with asbestosis, 154 subjects with MM and 149 subjects with no asbestos disease. The NLRP3 rs35829419 and CARD8 rs2043211 polymorphisms were determined using real-time PCR-based methods. In the statistical analysis, standard descriptive statistics was followed by univariate and multivariate logistic regression modelling. Results: Asbestos exposure (medium and high vs low) was associated with the risk for each studied asbestos-related disease. An increased risk of pleural plaques was found for CARD8 rs2043211 at + TT genotypes (OR = 1.48, 95% CI 1.01-2.16, p = 0.042). When the analysis was performed for MM patients as cases, and pleural plaques patients as controls, a decreased MM risk was observed for carriers of CARD8 rs2043211 TT genotype (OR = 0.52, 95% CI 0.27-1.00, p = 0.049). The interactions between NLRP3 rs35829419 and CARD8 rs2043211 genotypes did not influence the risk of any asbestos-related disease. However, when testing interactions with asbestos exposure, a decreased risk of asbestosis was found for NLRP3 CA+AA genotypes (OR = 0.09, 95% CI 0.01-0.60, p = 0.014). Conclusions: The results of our study suggest that NLRP3 and CARD8 polymorphisms could affect the risk of asbestos-related diseases.
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article language: English
document type: Original Scientific Paper
PMC ID: PMC7282242
DOI: 10.2478/jomb-2019-0025
published in SCIndeks: 10/01/2020
peer review method: double-blind
Creative Commons License 4.0

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