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2019, vol. 64, br. 4, str. 413-423
Genetički polimorfizam β-Laktoglobulina kod domaćih nigerijskih rasa koza
Federal University of Technology, Department of Animal Production, Minna, Niger State, Nigeria

e-adresaacheneje.egena@futminna.edu.ng
Ključne reči: polimorfizam; koze; lokus gena; PCR-RFLP; β-Laktoglobulin
Sažetak
Ispitivan je polimorfizam β-Laktoglobulina (β-LG) kod tri nigerijske rase koza: zapadnoafričkе patuljastе, sahel i crvenе sokoto kozе, korišćenjem metoda reakcije lančanog umnožavanja-polimorfizma dužine slučajnih delova (engl. Random Fragment Length Polymorphism - PCR-RFLP). Restrikcione endonukleaze korišćene u istraživanju uključivale su Rsal odnosno Mspl. Rezultati su otkrili postojanje samo jedne polimorfne varijante (alel A) sa frekvencijom gena od 1,0 kod sve tri rase koza koje su proučavane. Pojačani proizvodi uočeni su kod 120 bp, dok je restrikciona digestija sa endonukleazom Rsal otkrila samo jedan genotip kod lokusa β-LG. Zaključeno je da postoji odsustvo polimorfizma kod lokusa β-LG koza koje su ispitivane.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.2298/JAS1904413E
objavljen u SCIndeksu: 05.02.2020.
metod recenzije: dvostruko anoniman
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