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2013, vol. 2, br. 2, str. 64-70
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Analiza rezidua metabolita nitrofurana u životinjskoj plazmi primenom ultra-visokopritisne tečne hromatografije sa masenom spektrometrijom - podrška domaćoj mesnoj industriji
Residue analyses of nitrofuran metabolites in animal plasma by UHPLC-MS/MS: A support to domestic meat industry
aTeagasc Food Research Centre Ashtown, Dublin, Irska bUniverzitet u Novom Sadu, Tehnološki fakultet, Srbija
e-adresa: macanski@tf.uns.ac.rs
Sažetak
Pošto je primena nitrofurana zabranjena pri uzgoju životinja za klanje u Evropskoj Uniji (EU), ova jedinjenja se nalaze u Aneksu IV regulative 2377/90. Ne postoji prihvaćen limit za njihovo prisustvo u mesu, ili proizvodima životinjskog porekla. Zvanični markeri za kontaminaciju nitrofuranima iz krvne plazme su: 3-amino-2-oksazolidinon - AOZ, 3-amino-5-morfolinometil-2oksazolidinon - AMOZ, 1-aminohidantoin - AHD i semikarbazid - SEM. Ranije izvedena validacija metode primenom UHPLC-MS/MS (ultra-visoko pritisna tečna hromatografija sa masenom spektrometrijom) (in house) za određivanje metabolita nitrofurana, primenjena je na uzorcima krvi životinja za klanje iz Vojvodine. Uzorci (5 uzoraka svinje i 5 uzoraka goveda) su pripremljeni u Laboratoriji za analizu mesa, Tehnološkog fakulteta (Novi Sad, Srbija) i poslati u Teagasc Food research centre (Republika Irska), gde su analizirani. Validacija je izvedena u opsegu od 0.2 do 5 μg kg-1 primenom fortifikovane plazme životinja. CCα i CCβ vrednosti su određene po zahtevima EU. Rezultati pokazuju da su svi uzorci bili negativni na prisustvo AHD, AOZ i AMOZ. U 3 uzorka plazme svinja detektovano je prisustvo SEM. Ovaj rad unapređuje potencijal za izvoz domaćih životinja u EU, putem demonstrate kapaciteta za prepoznavanje i kvantifikaciju rezidua zabranjenih antibiotika u krvi u skladu sa EU legislativom. Pružena je podrška razvoju domaće industrije i potencijalnom izvozu proizvoda od mesa uz savremeni monitoring.
Abstract
Since NF application is prohibited in the breeding of pre-slaughter animals in the EU, these compounds are contained in Annex IV of the Regulation 2377/90. There is no accepted limit for their presence in the animal meat or meat products. Official markers of NF contamination from blood plasma are: 3-amino-2-oxazo-lidinone - AOZ, 3-amino-5-morpholinomethyl-1,3-oxazolidon-2-one - AMOZ, 1-aminohydantoin - AHD and semicarbazide-SEM. Previously carried out validation of the method using UHPLC/MS/MS (ultra-high performance liquid chromatography-tandem mass-spectrometry) (in house) for determining NF metabolites was also applied on the blood samples of pre-slaughter animals from Vojvodina. The samples were prepared in the Meat Analysis Laboratory, Faculty of Technology (Novi Sad, Serbia), and sent to the Teagasc Food Research Center (Republic of Ireland), where they were analyzed. The method was validated from 0.2-5 μg kg-1 using fortified animal plasma. CCα and CCβ values were determined according to EU requirements. The results showed that all samples were negative in terms of the presence of AHD, AOZ and AMOZ. SEM presence was detected in three samples of pig plasma. The paper improves the potential for the export of domestic animals in the EU by demonstrating the capacities for recognizing and quantification of residues of prohibited antibiotics in blood, in accordance with EU legislation. Modern monitoring methods were used in support of the development of domestic industry and the potential export of meat products.
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