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Zaštita bilja
2013, vol. 64, br. 3, str. 125-133
Otpornost korovskih populacija u voćnjacima na glifosat
Krga Irenaa ORCID, Pavlović Danijelab ORCID, Anđelković Anac, Đurović Sanjab ORCID, Marisavljević Draganab ORCID
aUniverzitet u Beogradu, Biološki fakultet, Srbija
bInstitut za zaštitu bilja i životnu sredinu, Beograd, Srbija
cnema

e-adresa: dulekaca@yahoo.com
Projekat:
Razrada integrisanog upravljanja i primene savremenih principa suzbijanja štetnih organizama u zaštiti bilja (MPNTR - 31018)

Sažetak
Danas, u Svetu i kod nas, za suzbijanje korova u voćnjacima najviše se koriste herbicidi na bazi glifosata, glufosinat-amonijuma, dikvata i drugi. Intenzivna primena glifosata uticala je na pojavu rezistentnih korovskih vrsta što za posledicu ima smanjenu efikasnost. U našoj zemlji površine pod voćnim zasadima iznose 224 hiljade hektara što svakako ukazuje na veliku upotrebu herbicida i mogućnost da je došlo do razvoja rezistentnih populacija korova. Zbog toga su sakupljana semena nekoliko korovskih vrsta sa površina gde je bila višegodišnja intenzivna primena glifosata u voćnjacima (Inđija, Brestovac, Šabac, Vršac, Sombor, Glogonjski Rit, Padinska Skela, Kosančić i Surčin). Biljke su gajene u kontrolisanim uslovima i na otvorenom polju. Biljni materijal je nakon uzorkovanja usitnjen u tečnom azotu i ekstrakcija šikiminske kiseline je urađena pomoću hlorovodonične kiseline (1 g biljnog materijala + 5 ml 1M HCL). Nakon 24 h očitavan je sadržaj šikiminske kiseline na tečnom hromatografu (HPLC). Analiza dobijenih rezultata je pokazala da su vrste Amaranthus retroflexus (lok. Šabac), Abutilon theophrasti (lok. Brestovac) i korovski Helianthus annuus (lok. G. Rit) razvile određeni stepen rezistentnosti prema glifosatu.
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