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2016, vol. 70, iss. 5-6, pp. 163-174
Boar and season effects on some parameters of semen fertilizing potential
aScientific Institute of Veterinary Medicine 'Novi Sad', Novi Sad
bUniversity of Novi Sad, Faculty of Agriculture, Department of Animal Science
cUniversity of Novi Sad, Faculty of Agriculture, Department of veterinary medicine
dUniversity of Belgrade, Faculty of Veterinary Medicine
Keywords: protein content; fertility of sperm; boar; season
In order to determine the more accurate fertile potential of sperm, it seems that the conventional parameters of boar semen quality (the ejaculate volume, sperm concentration, progressive motility, percentage of live sperm and of those with intact acrosomal morphology) are insufficient. Since recently, there have been numerous studies proving that protein concentration in sperm plasma has high positive correlation with boar fertile potential. The research objective was to determine the effect of boars as well as the season on the variation of protein content in the sperm plasma. For the research there were used spermal fractions of 2 boars with high (V-boar) and 2 boars with low (N-boar) protein content in spermal plasma. The ejaculates of boars were taken once a week, for a month, during one year (4 *12 = 48 ejaculates per boar). For protein analysis in the spermal plasma, the samples were prepared by centrifugation. The ejaculate volume, protein concentration and progressive motility varied considerably (p < 0.05 or p < 0.01) among the boars as well as in one and the same boar. The variations of the same parameters were determined also during both warm and cold season. On the other hand, protein concentration was rather constant, and in V-boars (ranged from 4 to 4.5%) while in N-boars (ranged from 2.3 to 2.6%). The season did not significantly affect (p > 0.01) protein content in sperm plasma (V-boars: 4 to 4.5% in warm and cold season; N-boars: 2.3 do 2.6% in warm and 2.3 to 2.5% in cold season). The obtained results showed that measurement of protein content in boar sperm plasma could be a useful method for their ranking, based on fertile potential of fresh semen.


article language: Serbian
document type: Original Paper
DOI: 10.2298/VETGL1606163A
published in SCIndeks: 13/01/2017

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