• citations in SCIndeks: [2]
  • citations in CrossRef:0
  • citations in Google Scholar:[]
  • visits in previous 30 days:1
  • full-text downloads in 30 days:1


article: 3 from 12  
Back back to result list
2011, vol. 65, iss. 4, pp. 473-477
Antioxidant activity of ultrasonic extracts of leek Allium porrum L.
aUniversity of Kragujevac, Faculty of Agronomy, Čačak, Serbia
bUniveristy of Niš, Faculty of Science, Serbia
Integrating biotechnology approach in breeding vegetable crops for sustainable agricultural systems (MESTD - 31059)

Keywords: ultrasonic extracts; leek; stem; leaf; antioxidant
This study was aimed at evaluating the antioxidant activity and efficacy of the ethanolic extract of the ultrasonic extracts of leek Allium porrum L. Ethanolic (50 vol.%) extracts of edible leek parts (stem and leaf) were prepared by ultrasound-assisted extraction, which was followed by evaluation of total phenols, flavonoids and antioxidant activity. Total phenols were determined using the modified Folin-Ciocalteu method. Antioxidant activity was assessed by scavenging the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). The results of antioxidant activity were compared with control antioxidants: vitamin C and BHT. The higher content of phenols (69.46 mg GAE/g dry extract) and flavonoids (33.53 mg CE/g dry extract) was found in the ethanolic extract of leek stem. The measured values of IC50 were 98.90 and 61.05 μg/mL for the ethanolic extract of leek leaf and stem, respectively.
*** (1984) Pharmacopoeia Jugoslavica. Belgrade: National Institute for Health Protection, Editio Quarta, vol. 1, 124-128
Anthony, M.S., Clarkson, T.B., Bullock, B.C., Wagner, J.D. (1997) Soy Protein Versus Soy Phytoestrogens in the Prevention of Diet-Induced Coronary Artery Atherosclerosis of Male Cynomolgus Monkeys. Arteriosclerosis, Thrombosis, and Vascular Biology, 17(11): 2524-2531
Arts, I.C.W., van de Putte, B., Hollman, P.C.H. (2000) Catechin Contents of Foods Commonly Consumed in The Netherlands. 1. Fruits, Vegetables, Staple Foods, and Processed Foods. Journal of Agricultural and Food Chemistry, 48(5): 1746-1751
Basile, A., Sorbo, S., Giordano, S., Ricciardi, L., Ferrara, S., Montesano, D., Clifford, M.N. (1999) Chlorogenic acids and other cinnamates - nature, occurrence and dietary burden. Journal of the Science of Food and Agriculture, 79(3): 362-372
Brighente, I.M.C., Dias, M., Verdi, L.G., Pizzolatti, M.G. (2007) Antioxidant Activity and Total Phenolic Content of Some Brazilian Species. Pharmaceutical Biology, 45(2): 156-161
Cassidy, A., Hanley, B., Lamuela-Raventos, R.M. (2000) Isoflavones, lignans and stilbens - origins, metabolism and potential importance to human health. J Sci Food Agric, 80, str. 1044-1062
Felter, H.W. (1922) The eclectic materia medica, pharmacology and therapeutics
Harborne, J.B., Williams, C.A. (2000) Advances in flavonoid research since 1992. Phytochemistry, 55(6): 481-504
Howard, B.V., Kritchevsky, D. (1997) Phytochemicals and cardiovascular disease. A statement for healthcare professionals from the American Heart Association. Circulation, 95(11): 2591-3
Kumarasamy, Y., Byres, M., Cox, P.J., Jaspars, M., Nahar, L., Sarker, S.D. (2007) Screening seeds of some Scottish plants for free radical scavenging activity. Phytotherapy research, 21(7): 615-21
Lakenbrink, C., Lapczynski, S., Maiwald, B., Engelhardt, U.H. (1996) Flavonoids and other polyphenols in consumer brews of tea and other caffeinated beverages. J. Agric. Food. Chem, 48, str. 2848-2852
Ness, A.R., Powles, J.W. (1997) Fruit and vegetables, and cardiovascular disease: A review. Int J Epidemiol, 26(1): 1-13
Onyilagha, J., Grotewold, E. (2004) The biology and structural distribution of surface flavonoids. Recent Res. Devel. Plant Sci, 2, str. 53-71
Prieto, P., Pineda, M., Aguilar, M. (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical biochemistry, 269(2): 337-41
Rates, S. (2001) Plants as source of drugs. Toxicon, 39(5): 603-613
Shahidi, F., Naczk, M. (1995) Food phenolics, sources, chemistry, effects, applications. Lancaster: Technomic Publishing
Sosulski, F., Krygier, K., Hogge, L. (1982) Free, esterified, and insoluble-bound phenolic acids, composition of phenolic acids in cereal and potato flours. Journal of Agricultural and Food Chemistry, 30, 337-340
Takao, T., Watanabe, N., Yagi, I., Sakata, K. (1994) A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci. Biotech. Biochem, 58, 1780-1783
Tomas-Barberan, F., Clifford, M. (2000) Flavanones, chalcones and dihydrochalcones: Nature, occurrence and dietary burden. Journal of the Science of Food and Agriculture, 80(7): 1073-1080
Vinatoru, M., Toma, M., Radu, O., Filip, P.I., Lazurca, D., Mason, T.J. (1997) The use of ultrasound for the extraction of bioactive principles from plant materials. Ultrason Sonochem, 4(2): 135-9
Zhu, Q.Y., Zhang, A., Tsang, D., Huang, Y., Chen, Z. (1997) Stability of Green Tea Catechins. Journal of Agricultural and Food Chemistry, 45(12): 4624-4628


article language: English
document type: Scientific Paper
DOI: 10.2298/HEMIND110301033M
published in SCIndeks: 07/09/2011

Related records

No related records