To ensure high and stable seed yields, in regions with semi humid climate, it is necessary to prevent the luxuriant growth of alfalfa plants and their subsequent lodging. The most effective method of controlling rank growth in alfalfa is cutting. Effect of cutting schedule on alfalfa seed yield was studied in four-year experiments (2001-2004). Four cutting schedules with variable dates of initial cutting were tested: first cutting for hay was carried out on May 5 (budding) in the c1 schedule, on May 15 (start of flowering) in the c2 schedule, on May 25 (full flowering) in the c3 schedule, and on May 5 and June 5 in the c4 schedule. Second regrowth was used for seed production in the c1-c3 schedules, and third regrowth in the c4 schedule. The study involved the seven most widely grown alfalfa cultivars in Serbia, six domestic and one French. The single late cut (c3 schedule) achieved the best balance among the yield components and thus the highest seed yield (468 kg/ha). Significant positive correlations were found between seed yield on one side and the number of fertile shoots per unit area and the number of pods per inflorescence on the other, r = 0.608 and r = 0.837, respectively.