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2018, vol. 7, br. 2, str. 31-40
Antioksidativna i antimikrobna aktivnost vodenog ekstrakta dimnjače (Fumaria officinalis L.)
aUniverzitet u Nišu, Tehnološki fakultet, Leskovac
bIstraživačko-razvojni centar 'ALFATEC' doo, Niš

e-adresastanojevic@tf.ni.ac.rs
Projekat:
Biljni i sintetički bioaktivni proizvodi novije generacije (MPNTR - 34012)

Ključne reči: Fumaria officinalis L.; vodeni ekstrakt dimnjače; antioksidativna aktivnost; antimikrobna aktivnost; ruzmarinska kiselina
Sažetak
U istraživanju je korišćen nadzemni deo dimnjače (Fumaria officinalis L.) kao ekstrakcioni materijal. Biljni materijal je preliven ključalom vodom pri hidromodulu 1:20 m/v i ostavljen da stoji 90 min na sobnoj temperaturi u cilju dobijanja vodenog ekstrakta. Identifikacija bioaktivnih komponenti u ekstraktu izvršena je primenom UHPLC-DAD-ESI-MS analize. Sadržaj ukupnih fenola i ukupnih flavonoida određen je spektrofotometrijski metodom po Folin-Ciocalte-u i metodom sa AlCl3, respektivno. Antioksidativna aktivnost je takođe određena spektrofotometijski primenom DPPH i ABTS testa. Antimikrobna aktivnost ekstrakta određena je diskdifuzionom metodom na sledeće patogene mikroorganizme: Candida albicans, Proteus vulgaris, Pseudomonas aeruginosa, Bacillus subtilis, Staphylococcus aureus, Escherichia coli i Klebsiella pneumoniae. U ekstraktu je registrovano sedam komponenti, dok su identifikovane dve, kafeinska i ruzmarinska kiselina. Najzastu­pljenija komponenta u ekstraktu je ruzmarinska kiselina (16,7 g/100 g suvog ek­strakta). Sadržaj ukupnih fenola je bio 77,67 mg EGK/g suvog ekstrakta, dok je sadržaj ukupnih flavonoida bio 21,21 mg ER/g suvog ekstrakta. Koncentracija ekstrakta potrebna za neutralisanje 50% od početne koncentracije DPPH radikala nakon 30 min inkubacije, 20 min inkubacije i neposredno nakon dodavanja DPPH radikala iznosila je 7,38; 8,18 i 48,6 μg/cm3, respektivno, dok je potrebna koncentracija za neutralisanje 50% od početne koncentracije ABTS radikala iznosila 96,0 μg/cm3. Ekstrakt je pokazao najbolju antioksidativnu aktivnost na gljivu Candida albicans. Prezentovani rezultati pokazuju da dobijeni vodeni ekstrakt nadzemnog dela dimnjače predstavlja potencijalni izvor prirodnih antioksidanasa i antimikrobnih agenasa.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/SavTeh1802031S
objavljen u SCIndeksu: 03.01.2019.

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