This paper presents the methods and techniques for assessing the challenges, risks and threats, which can be used in the Republic of Serbia for the purpose of making strategic and planning documents by the various entities in the field of safety. Aside from the terminological demarcation, using the combined method, the paper demonstrates various practical applications of the methods and techniques of assessing challenges, risks and threats. The main objective of this paper is to estimate the direct extension of existing knowledge by reviewing the relatively new empirical methods used in the country and the world. However, the paper draws attention of the professional community to the fact that studying this problem revealed high dependency assessment challenges, risks and threats to the security of the determinants, components and factors of different forms of security: global, regional, national, human, economic, social, energy, environmental, individual and so on, which, objectively, complicates the procedure of evaluating and quantifying the degree of security threats. It was also noted that in the application of exact techniques and tools in order to quantify, there are objective difficulties due to the complex nature of the phenomena described in practice, especially in cases of the so-called measurement of threats, causes, contents and direction of security threats and the like. But regardless of these problems, it is possible to create tables and scales to quantify the challenges, risks and threats, and they have been included in this study as an integral part which can provide substantial assistance in the implementation of qualitative and quantitative methods of assessment in practice.