Of the bacteria of the genus Staphylococcus, the aureus variety is almost always pathogenic. All pathogenic Staphylococcus are excreting coagulase, so this test is taken to determine the Staphylococcus virulence. Staphylococcus is very widespread in nature and are found in water, air and dust. They are very resistant to high concentrations of salt and sugar, and are often found in foods. The pathogens of Staphylococcus aureus are often found to be part of a normal flora in the nasal cavity of healthy people of 20-60% of the population. Pathogenic Staphylococcus secrete enterotoxin which is thermostable. When, under favorable conditions, Staphylococcus is multipling in food, the created enterotoxin causes food poisoning in humans (Intoxicatio alimentaris). That is why, for certain categories of employees, medical examinations that include bacteriological examination of the swabs of the throat and nose on Staphylococcus aureus, coagulase are positive. We have processed microbiological reports of the nose swabs of certain categories of employees District of Pirot for the period from 2014. to 2016. and observed whether these results can be explained by the climatic conditions of certain seasons in which these checks were carried out. The highest percentage of positive Staphylococcus findings in the nasal swab was during the summer (7.6%), then winter (7.37%), autumn (6.85%) and the smallest during the spring (5.17%). This result is explained by the effect of factors that affect the occurrence of the disease (low winter temperatures or artificial low temperature formation in relation to the outside environment during the summer, cold weather, consumption of cold drinks, ice cream, easy dressing, use of air conditioners, dealing with a certain jobs that requires low temperature operation). These factors are most apparent at very low or very high temperatures, i.e. during winter and summer.