• citations in SCIndeks: 0
  • citations in CrossRef:0
  • citations in Google Scholar:[]
  • visits in previous 30 days:4
  • full-text downloads in 30 days:2


article: 4 from 14  
Back back to result list
2010, vol. 60, iss. 4, pp. 401-410
Biochemical markers of bone metabolism in dairy cows with milk fever
University of Ljubljana, Veterinary Faculty, Clinic for ruminants, Ljubljana, Slovenia
Bone metabolism was investigated in 8 healthy and 12 dairy cows suffering from milk fever (MF) in intensive dairy production. Blood samples were taken within 48 hours after calving in healthy cows and before treatment in cows with MF. Bone and mineral metabolism were evaluated by measuring blood serum bone resorption biomarker Cterminal telopeptide of type I collagen (CTx) and bone formation biomarker bone-specific alkaline phosphatase (bALP) beside the classical panel: total calcium (Ca), inorganic phosphate (iP), magnesium (Mg) and alkaline phosphatase (ALP). The results were statistically analyzed and compared between the two groups. Mean Ca value in cows with milk fever was 1.01±0.29 mmol/L and in healthy cows it was significantly (p<0.05) higher (1.94± 0.04 mmol/L). All the cows were also hypophosphatemic with lower phosphorus values (p<0.05) in MF cows. Some cows with MF were also hypermagnesemic, but the difference in Mg concentrations between the two groups was not significant. Mean total ALP and bALP activity were higher in cows with MF (65±17.7 U/L and 21.3±8.5 U/L, respectively) than in healthy cows (55.9±7.0 U/L and 20.5±8.9 U/L, respectively). The mean concentration of blood serum CTx was lower in cows with MF (0.212±0.091 ng/L) than in healthy cows (0.417± 0.252 ng/L), but as for bALP not significantly.
Allen, M.J. (2003) Biochemical markers of bone metabolism in animals: Uses and limitations. Vet Clin Pathol, 32(3): 101-13
Beighle, D.E. (1999) The effect of gestation and lactation on bone calcium, phosphorus and magnesium in dairy cows. Journal of the South African Veterinary Association, 70(4): 142-6
Bigras-Poulin, M., Tremblay, A. (1998) An epidemiological study of calcium metabolism in non-paretic postparturient Holstein cows. Prev Vet Med, 35(3): 195-207
Christenson, R.H. (1997) Biochemical markers of bone metabolism: An overview. Clinical biochemistry, 30(8): 573-93
de Garis, P.J., Lean, I.J. (2008) Milk fever in dairy cows: A review of pathophysiology and control principles. Veterinary journal, 176(1): 58-69
Filipović, N., Stojević, Z., Zdelar-Turk, M., Kušec, V. (2008) Plasma parathyroid hormone-related peptide and bone metabolism in periparturient dairy cows. Acta Veterinaria Hungarica, 56(2): 235
Fontenot, J.P., Allen, V.G., Bunce, G.E., Goff, J.P. (1989) Factors influencing magnesium absorption and metabolism in ruminants. Journal of animal science, 67(12): 3445-55
Gašperlin, B., Zadnik, T., Jazbec, I., Žust, J. (2002) Effects of dietary cation-anion differences on serum calcium, phosphorus and magnesium concentrations in periparturient dairy cows. Slov Vet Res, 39, 3/4, 215-25
Goff, J.P. (2000) Pathophysiology of calcium and phosphorus disorders. Veterinary clinics of North America. Food animal practice, 16(2): 319-37, vii
Goff, J.P. (2004) Macromineral disorders of the transition cow. Veterinary Clinics of North America Food Animal Practice, 20(3): 471-94
Goff, J.P. (2008) The monitoring, prevention, and treatment of milk fever and subclinical hypocalcemia in dairy cows. Veterinary journal, 176(1): 50-7
Holtenius, K., Ekelund, A. (2005) Biochemical markers of bone turnover in the dairy cow during lactation and the dry period. Research in veterinary science, 78(1): 17-9
Horst, R.L., Goff, J.P., Reinhardt, T.A. (1994) Calcium and vitamin D metabolism in the dairy cow. Journal of dairy science, 77(7): 1936-51
Horst, R.L., Goff, J.P., Mccluskey, B.J. (2003) Prevalence of subclinical hypocalcemia in US dairy operations. J Dairy Sci, 86 (Suppl. 1), 247
Iwama, Y., Kamiya, M., Tanaka, M., Shioya, S. (2004) The change of dry matter intake, milk yield and bone turnover in primiparous cows compared to multiparous cows during early lactation. Animal Science Journal, 75(3): 213
Jazbec, I., Gregorovič, V., Bekš, L. (1970) Učinek velikih doz kristaliničnega vitamina D na nihanje koncentracije serumskega Ca in anorganskega P pri kravah, neposredno ogroženih od hipokalcemične puerperalne pareze. Zb Bioteh fak Univ v Ljubljani, 7, 59-69
Kossaibati, M.A., Esslemont, R.J. (1997) The costs of production diseases in dairy herds in England. Vet J, 154(1): 41-51
Lappeteläinen, R., Lappetelainen, E., Hassinen, T., Mahl, M., Pirskanen, A., Maenpaa, P.H. (1993) Biochemical indicators of bone metabolic activity in bovine periparturient hypocalcemia. Journal of Veterinary Medicine Series A, 40(1-10): 67
Larsen, T., Moller, G., Bellio, R. (2001) Evaluation of clinical and clinical chemical parameters in periparturient cows. J Dairy Sci, 84(7): 1749-58
Liesegang, A., Sassi, M.L., Risteli, J., Eicher, R., Wanner, M., Riond, J.L. (1998) Comparison of bone resorption markers during hypocalcemia in dairy cows. Journal of dairy science, 81(10): 2614-22
National Research Council (2001) Nutrient requirements of domestic animals: Nutrient requirements of dairy cattle. Washington, DC: National Academy Press, 7th rev. ed
Oetzel, G.R. (1988) Parturient paresis and hypocalcemia in ruminant livestock. Vet Clin North Am Food Anim Pract, 4(2): 351-64
Riggs, B., Khosla, S., Melton, L. (2002) Sex steroids and the construction and conservation of the adult skeleton. Endocr Rev, 23(3): 279-302
Riond, J.L., Kocabagli, N., Spichiger, U.E., Wanner, M. (1995) The concentration of ionized magnesium in serum during the periparturient period of non-paretic dairy cows. Veterinary research communications, 19(3): 195-203
Roche, J.R. (2003) The incidence and control of hypocalcaemia in pasture based systems. Acta Vet Scand, Suppl 97, 141-4
Sechen, S.J., Bremel, R.D., Jorgensen, N.A. (1988) Prolactin, estradiol, and progesterone changes in paretic and nonparetic cows during the periparturient period. American journal of veterinary research, 49(3): 411-6
Starič, J., Nemec, M., Zadnik, T. (2008) Dynamics of bone metabolism in high pregnant heifers and first lactation cows. in: Middle European buiatrics congress (9th), Budapest, Hungary, 6-11 July, Proceedings: within the framework of the 25th world buiatrics congress, Budapest: Hungarian association for buiatrics, 85-6
Starič, J., Ježek, J., Klinkon, M., Nemec, M., Zadnik, T. (2008) Bone tissue metabolism in cattle. Acta Agric Slov, Suppl, 2, 163-6


article language: English
document type: unclassified
DOI: 10.2298/AVB1004401S
published in SCIndeks: 14/09/2010

Related records