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2016, vol. 53, iss. 1, pp. 1-8
A rapid test for detection of tribenuron-methyl resistance in sunflower
aInstitute of Field and Vegetable Crops, Novi Sad, Serbia
bInstitute of Lowland Forestry and Environment, Novi Sad, Serbia
cUniversity of Belgrade, Faculty of Agriculture, Serbia

emaildragana.miladinovic@ifvcns.ns.ac.rs
Project:
Development of new varieties and production technology improvement of oil crops for different purposes (MESTD - 31025)

Abstract
A rapid laboratory test for detection of tribenuron-methyl resistant sunflower genotypes was developed. Four homozygous and four heterozygous tribenuron-methyl resistant hybrids, as well as a susceptible one were grown on MS medium supplemented with different concentrations of herbicide (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM and 4.0 µM) and with pH either 7 or 8. The effect of medium pH and herbicide concentration on above-ground part and root mass of sunflower seedlings was observed. The test enabled visual discrimination between resistant and susceptible genotypes in 5 days, and discrimination between homozygous and heterozygous resistant genotypes in 12 days. All tested herbicide concentrations and morphological parameters were found to be suitable for discrimination of tribenuron-methyl susceptible genotypes. The best pH and herbicide concentration combination and morphological parameter for discrimination between homozygous and heterozygous resistant sunflower genotypes were 3.0 µM at pH 7 and root mass, respectively.
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About

article language: English
document type: Original Scientific Paper
DOI: 10.5937/ratpov53-8412
published in SCIndeks: 06/05/2016
peer review method: double-blind
Creative Commons License 4.0

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