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Ratarstvo i povrtarstvo
2016, vol. 53, br. 1, str. 1-8
Brzi test za ispitivanje otpornosti suncokreta na tribenuron metil
Dimitrijević Aleksandraa ORCID, Imerovski Ivanaa ORCID, Miladinović Draganaa ORCID, Jocić Sinišaa ORCID, Cvejić Sandraa ORCID, Malidža Gorana ORCID, Kovačević Branislavb ORCID, Šurlan-Momirović Gordanac ORCID
aNaučni institut za ratarstvo i povrtarstvo, Novi Sad, Srbija
bInstitut za nizijsko šumarstvo i životnu sredinu, Novi Sad, Srbija
cUniverzitet u Beogradu, Poljoprivredni fakultet, Srbija

e-adresa: dragana.miladinovic@ifvcns.ns.ac.rs
Projekat:
Razvoj novih sorti i poboljšanje tehnologija proizvodnje uljanih biljnih vrsta za različite namene (MPNTR - 31025)

Ključne reči: biološka proba; Helianthus annuus L.; herbicidi; selekcija; sulfoniluree; tolerantnost
Sažetak
Razvijen je brzi laboratorijski test za detekciju genotipova suncokreta otpornih na tribenuron metil. Po četiri hibrida suncokreta homozigotno i heterozigotno otporna na tribenuron metil, kao i jedan neotporan hibrid su gajeni na MS podlozi sa pH vrednošću ili 7 ili 8 i sa različitim koncentracijama herbicida Express 50X, čija je aktivna materija tribenuron-metil (2.0 µM, 2.5 µM, 3.0 µM, 3.5 µM i 4.0 µM). Ispitivan je uticaj pH i koncentracije herbicida u podlozi na masu nadzemnog dela i korena klijanaca testiranih hibrida. Vizuelne razlike između otpornih i neotpornog hibrida su uočene nakon 5 dana kulture, dok su se razlike između homozigotnih i heterozigotnih otpornih hibrida pojavile nakon 12 dana kulture. Sve testirane koncentracije herbicida i testirani morfološki parametri su se pokazali kao dobri za identifikaciju neotpornih genotipova. Najbolja kombinacija pH i koncentracije herbicida za razlikovanje homozigotnih od heterozigotnih otpornih genotipova je bila 3.0 µM na pH 7, a morfološki parametar masa korena.
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O članku

jezik rada: engleski
vrsta rada: izvorni naučni članak
DOI: 10.5937/ratpov53-8412
objavljen u SCIndeksu: 06.05.2016.
metod recenzije: dvostruko anoniman
Creative Commons License 4.0

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